Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System
Vascularization of tissues, organoids and organ-on-chip models has been attempted using endothelial cells. However, the cultured endothelial cells lack the capacity to interact with other somatic cell types, which is distinct from developing vascular cells in vivo. Recently, it was demonstrated that...
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2021-08-01
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| author | Yujin Ahn Ju-Hyun An Hae-Jun Yang Dong Gil Lee Jieun Kim Hyebin Koh Young-Ho Park Bong-Seok Song Bo-Woong Sim Hong J. Lee Jong-Hee Lee Sun-Uk Kim |
| author_facet | Yujin Ahn Ju-Hyun An Hae-Jun Yang Dong Gil Lee Jieun Kim Hyebin Koh Young-Ho Park Bong-Seok Song Bo-Woong Sim Hong J. Lee Jong-Hee Lee Sun-Uk Kim |
| author_sort | Yujin Ahn |
| collection | DOAJ |
| description | Vascularization of tissues, organoids and organ-on-chip models has been attempted using endothelial cells. However, the cultured endothelial cells lack the capacity to interact with other somatic cell types, which is distinct from developing vascular cells in vivo. Recently, it was demonstrated that blood vessel organoids (BVOs) recreate the structure and functions of developing human blood vessels. However, the tissue-specific adaptability of BVOs had not been assessed in somatic tissues. Herein, we investigated whether BVOs infiltrate human cerebral organoids and form a blood–brain barrier. As a result, vascular cells arising from BVOs penetrated the cerebral organoids and developed a vessel-like architecture composed of CD31<sup>+</sup> endothelial tubes coated with SMA<sup>+</sup> or PDGFR<sup>+</sup> mural cells. Molecular markers of the blood-brain barrier were detected in the vascularized cerebral organoids. We revealed that BVOs can form neural-specific blood-vessel networks that can be maintained for over 50 days. |
| first_indexed | 2024-03-10T08:55:16Z |
| format | Article |
| id | doaj.art-237725e7f10841d3bdb4d1ba4d04d108 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T08:55:16Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-237725e7f10841d3bdb4d1ba4d04d1082023-11-22T07:10:49ZengMDPI AGCells2073-44092021-08-01108203610.3390/cells10082036Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like SystemYujin Ahn0Ju-Hyun An1Hae-Jun Yang2Dong Gil Lee3Jieun Kim4Hyebin Koh5Young-Ho Park6Bong-Seok Song7Bo-Woong Sim8Hong J. Lee9Jong-Hee Lee10Sun-Uk Kim11Futuristic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaFuturistic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaFuturistic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaFuturistic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaFuturistic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaFuturistic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaFuturistic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaFuturistic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaFuturistic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaCollege of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 28644, KoreaDepartment of Functional Genomics, KRIBB School of Bioscience, Korea University of Science and Technology, Daejeon 34113, KoreaFuturistic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, KoreaVascularization of tissues, organoids and organ-on-chip models has been attempted using endothelial cells. However, the cultured endothelial cells lack the capacity to interact with other somatic cell types, which is distinct from developing vascular cells in vivo. Recently, it was demonstrated that blood vessel organoids (BVOs) recreate the structure and functions of developing human blood vessels. However, the tissue-specific adaptability of BVOs had not been assessed in somatic tissues. Herein, we investigated whether BVOs infiltrate human cerebral organoids and form a blood–brain barrier. As a result, vascular cells arising from BVOs penetrated the cerebral organoids and developed a vessel-like architecture composed of CD31<sup>+</sup> endothelial tubes coated with SMA<sup>+</sup> or PDGFR<sup>+</sup> mural cells. Molecular markers of the blood-brain barrier were detected in the vascularized cerebral organoids. We revealed that BVOs can form neural-specific blood-vessel networks that can be maintained for over 50 days.https://www.mdpi.com/2073-4409/10/8/2036vascularizationblood vessel organoidcerebral organoidblood-brain barriervessel structureorganoids |
| spellingShingle | Yujin Ahn Ju-Hyun An Hae-Jun Yang Dong Gil Lee Jieun Kim Hyebin Koh Young-Ho Park Bong-Seok Song Bo-Woong Sim Hong J. Lee Jong-Hee Lee Sun-Uk Kim Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System Cells vascularization blood vessel organoid cerebral organoid blood-brain barrier vessel structure organoids |
| title | Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System |
| title_full | Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System |
| title_fullStr | Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System |
| title_full_unstemmed | Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System |
| title_short | Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System |
| title_sort | human blood vessel organoids penetrate human cerebral organoids and form a vessel like system |
| topic | vascularization blood vessel organoid cerebral organoid blood-brain barrier vessel structure organoids |
| url | https://www.mdpi.com/2073-4409/10/8/2036 |
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