gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis
Obesity is the main cause of metabolic complications. Fatty liver infiltration is a companion of obesity. NAFLD is associated with impaired energy metabolism with an excess of nutrients. Mitochondrial dynamics are important for the regulation of energy balance, which regulates mitochondrial function...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/2/396 |
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| author | Daria Shunkina Anastasia Dakhnevich Egor Shunkin Olga Khaziakhmatova Valeria Shupletsova Maria Vulf Alexandra Komar Elena Kirienkova Larisa Litvinova |
| author_facet | Daria Shunkina Anastasia Dakhnevich Egor Shunkin Olga Khaziakhmatova Valeria Shupletsova Maria Vulf Alexandra Komar Elena Kirienkova Larisa Litvinova |
| author_sort | Daria Shunkina |
| collection | DOAJ |
| description | Obesity is the main cause of metabolic complications. Fatty liver infiltration is a companion of obesity. NAFLD is associated with impaired energy metabolism with an excess of nutrients. Mitochondrial dynamics are important for the regulation of energy balance, which regulates mitochondrial function, apoptosis, and mitophagy. The aim of this study was to investigate the effect of gp130 on the components of mitochondrial dynamics in a cellular model of steatohepatitis. Addition of IL-6/gp130 contributed to an increase in the percentage of live cells and a decrease in the percentage of dead and apoptotic cells. Addition of IL-6/gp130 increased the expression of NF-kB1 gene and mitochondrial dynamics markers (MFN2 and TFAM) in HepG2 with tBHP/Oleic. Addition of IL-6 or gp130 reduced the expression of cytoprotector genes (HSF1 and HSP70) in HepG2 cell cultures with tBHP/Oleic. Increased mitochondrial dynamics gene activity protected against HepG2 cell death in the steatohepatitis model. Trans-signaling resulted in increased TFAM and MAPLC3B, and decreased DNM1L gene expression in HepG2 with tBHP/Oleic. |
| first_indexed | 2024-03-11T09:06:41Z |
| format | Article |
| id | doaj.art-237a311661b94212bc32109048d092c1 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T09:06:41Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-237a311661b94212bc32109048d092c12023-11-16T19:17:37ZengMDPI AGBiomedicines2227-90592023-01-0111239610.3390/biomedicines11020396gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of SteatohepatitisDaria Shunkina0Anastasia Dakhnevich1Egor Shunkin2Olga Khaziakhmatova3Valeria Shupletsova4Maria Vulf5Alexandra Komar6Elena Kirienkova7Larisa Litvinova8Centre for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaCentre for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaCentre for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaCentre for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaCentre for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaCentre for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaCentre for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaCentre for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaCentre for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaObesity is the main cause of metabolic complications. Fatty liver infiltration is a companion of obesity. NAFLD is associated with impaired energy metabolism with an excess of nutrients. Mitochondrial dynamics are important for the regulation of energy balance, which regulates mitochondrial function, apoptosis, and mitophagy. The aim of this study was to investigate the effect of gp130 on the components of mitochondrial dynamics in a cellular model of steatohepatitis. Addition of IL-6/gp130 contributed to an increase in the percentage of live cells and a decrease in the percentage of dead and apoptotic cells. Addition of IL-6/gp130 increased the expression of NF-kB1 gene and mitochondrial dynamics markers (MFN2 and TFAM) in HepG2 with tBHP/Oleic. Addition of IL-6 or gp130 reduced the expression of cytoprotector genes (HSF1 and HSP70) in HepG2 cell cultures with tBHP/Oleic. Increased mitochondrial dynamics gene activity protected against HepG2 cell death in the steatohepatitis model. Trans-signaling resulted in increased TFAM and MAPLC3B, and decreased DNM1L gene expression in HepG2 with tBHP/Oleic.https://www.mdpi.com/2227-9059/11/2/396metabolic syndromenon-alcoholic fatty liver disease (NAFLD)IL-6gp130mitochondriaoxidative stress |
| spellingShingle | Daria Shunkina Anastasia Dakhnevich Egor Shunkin Olga Khaziakhmatova Valeria Shupletsova Maria Vulf Alexandra Komar Elena Kirienkova Larisa Litvinova gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis Biomedicines metabolic syndrome non-alcoholic fatty liver disease (NAFLD) IL-6 gp130 mitochondria oxidative stress |
| title | gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis |
| title_full | gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis |
| title_fullStr | gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis |
| title_full_unstemmed | gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis |
| title_short | gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis |
| title_sort | gp130 activates mitochondrial dynamics for hepatocyte survival in a model of steatohepatitis |
| topic | metabolic syndrome non-alcoholic fatty liver disease (NAFLD) IL-6 gp130 mitochondria oxidative stress |
| url | https://www.mdpi.com/2227-9059/11/2/396 |
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