Glycans as Potential Diagnostic Markers of Traumatic Brain Injury in Children
Diagnosing mild traumatic brain injury (TBI) in the acute setting is challenging due to the nonspecific and often transient or delayed symptoms. Further, the criteria for acute head imaging are frequently not fulfilled, which may lead to a missed diagnosis. A rapid test to diagnose TBI using body fl...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/13/13/2181 |
| _version_ | 1797591883449368576 |
|---|---|
| author | Mårten Kvist Lasse Välimaa Adrian Harel Sari Malmi Aleksi Tuomisto |
| author_facet | Mårten Kvist Lasse Välimaa Adrian Harel Sari Malmi Aleksi Tuomisto |
| author_sort | Mårten Kvist |
| collection | DOAJ |
| description | Diagnosing mild traumatic brain injury (TBI) in the acute setting is challenging due to the nonspecific and often transient or delayed symptoms. Further, the criteria for acute head imaging are frequently not fulfilled, which may lead to a missed diagnosis. A rapid test to diagnose TBI using body fluids would be highly useful. Urine and saliva samples were collected from 28 pediatric patients (mean [SD] age, eight years two months [four years three months]) with acute, clinically diagnosed mild TBI and 30 healthy volunteers at Satasairaala Hospital, Pori, Finland, over 11 months. The mean (SD) time from trauma to first sampling was 3 h 56 min (1 h 14 min). Samples were analyzed to determine the number of lectin-binding glycan molecules, indicating nerve tissue damage. The relative levels of several lectin-bound glycans were measured by fluorescence. Compared with healthy controls, the TBI group showed significant increases (<i>p</i> < 0.05, Wilcoxon rank-sum two-sided test) in nine glycans in the saliva, one glycan in the urine, and a significant decrease in seven glycans in the urine. These findings of potentially diagnostic glycans in body fluids after TBI warrant further research and may enable the development of a rapid body fluid-based point-of-care test to identify pediatric patients with TBI after a head injury. |
| first_indexed | 2024-03-11T01:43:44Z |
| format | Article |
| id | doaj.art-237a3cbbd8974215aa2d61db30c53729 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T01:43:44Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-237a3cbbd8974215aa2d61db30c537292023-11-18T16:21:08ZengMDPI AGDiagnostics2075-44182023-06-011313218110.3390/diagnostics13132181Glycans as Potential Diagnostic Markers of Traumatic Brain Injury in ChildrenMårten Kvist0Lasse Välimaa1Adrian Harel2Sari Malmi3Aleksi Tuomisto4Medicortex Finland Plc, 20520 Turku, FinlandMedicortex Finland Plc, 20520 Turku, FinlandMedicortex Finland Plc, 20520 Turku, FinlandDepartment of Pediatric Surgery, Satasairaala Hospital, 28500 Pori, FinlandDepartment of Pediatric Surgery, Satasairaala Hospital, 28500 Pori, FinlandDiagnosing mild traumatic brain injury (TBI) in the acute setting is challenging due to the nonspecific and often transient or delayed symptoms. Further, the criteria for acute head imaging are frequently not fulfilled, which may lead to a missed diagnosis. A rapid test to diagnose TBI using body fluids would be highly useful. Urine and saliva samples were collected from 28 pediatric patients (mean [SD] age, eight years two months [four years three months]) with acute, clinically diagnosed mild TBI and 30 healthy volunteers at Satasairaala Hospital, Pori, Finland, over 11 months. The mean (SD) time from trauma to first sampling was 3 h 56 min (1 h 14 min). Samples were analyzed to determine the number of lectin-binding glycan molecules, indicating nerve tissue damage. The relative levels of several lectin-bound glycans were measured by fluorescence. Compared with healthy controls, the TBI group showed significant increases (<i>p</i> < 0.05, Wilcoxon rank-sum two-sided test) in nine glycans in the saliva, one glycan in the urine, and a significant decrease in seven glycans in the urine. These findings of potentially diagnostic glycans in body fluids after TBI warrant further research and may enable the development of a rapid body fluid-based point-of-care test to identify pediatric patients with TBI after a head injury.https://www.mdpi.com/2075-4418/13/13/2181TBIlectinglycanbiomarkerconcussiontraumatic brain injury |
| spellingShingle | Mårten Kvist Lasse Välimaa Adrian Harel Sari Malmi Aleksi Tuomisto Glycans as Potential Diagnostic Markers of Traumatic Brain Injury in Children Diagnostics TBI lectin glycan biomarker concussion traumatic brain injury |
| title | Glycans as Potential Diagnostic Markers of Traumatic Brain Injury in Children |
| title_full | Glycans as Potential Diagnostic Markers of Traumatic Brain Injury in Children |
| title_fullStr | Glycans as Potential Diagnostic Markers of Traumatic Brain Injury in Children |
| title_full_unstemmed | Glycans as Potential Diagnostic Markers of Traumatic Brain Injury in Children |
| title_short | Glycans as Potential Diagnostic Markers of Traumatic Brain Injury in Children |
| title_sort | glycans as potential diagnostic markers of traumatic brain injury in children |
| topic | TBI lectin glycan biomarker concussion traumatic brain injury |
| url | https://www.mdpi.com/2075-4418/13/13/2181 |
| work_keys_str_mv | AT martenkvist glycansaspotentialdiagnosticmarkersoftraumaticbraininjuryinchildren AT lassevalimaa glycansaspotentialdiagnosticmarkersoftraumaticbraininjuryinchildren AT adrianharel glycansaspotentialdiagnosticmarkersoftraumaticbraininjuryinchildren AT sarimalmi glycansaspotentialdiagnosticmarkersoftraumaticbraininjuryinchildren AT aleksituomisto glycansaspotentialdiagnosticmarkersoftraumaticbraininjuryinchildren |