Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model
Abstract In recent years, studies have explored novel approaches for cell transplantation to enable annulus fibrosus (AF) regeneration of the intervertebral disc in particular for lumbar disc herniation. Nevertheless, successful engraftment of cells is structurally challenging, and no definitive met...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-06-01
|
| Series: | JOR Spine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jsp2.1050 |
| _version_ | 1828518970838220800 |
|---|---|
| author | Tadashi Nukaga Daisuke Sakai Jordy Schol Masato Sato Masahiko Watanabe |
| author_facet | Tadashi Nukaga Daisuke Sakai Jordy Schol Masato Sato Masahiko Watanabe |
| author_sort | Tadashi Nukaga |
| collection | DOAJ |
| description | Abstract In recent years, studies have explored novel approaches for cell transplantation to enable annulus fibrosus (AF) regeneration of the intervertebral disc in particular for lumbar disc herniation. Nevertheless, successful engraftment of cells is structurally challenging, and no definitive method has yet been established. This study investigated the potential of cell sheet technology to facilitate cell engraftment for AF repair. AF injury was induced by a 1 × 1 mm defect in rat tails after which AF cell sheets were transplanted. Its regenerative effects were compared to a nondegenerated and degeneration only conditions. Degenerative changes of the entire intervertebral disc were examined by disc height measurements, histology, and immunohistochemistry for 4‐, 8‐, and 12‐weeks post‐transplantation. Cell engraftment was confirmed by tracing PKH26 fluorescent dyed AF cells. In the transplant group, disc degeneration was significantly suppressed after 4, 8, and 12 weeks when compared with the degenerative group, as indicated by histological scoring and DHI observations. At 2 and 4 weeks after transplant, PKH26 positive cells could be detected in defect region and surrounding AF. The results suggest cell engraftment into AF tissue could be established by the cell sheet technology without additional scaffolding or adhesives. In short, AF cell sheets appear to be an effective and accessible tool for AF repair and to support intervertebral disc regeneration. |
| first_indexed | 2024-12-11T19:04:04Z |
| format | Article |
| id | doaj.art-237b15fc6a664706be885c0cc4f710ed |
| institution | Directory Open Access Journal |
| issn | 2572-1143 |
| language | English |
| last_indexed | 2024-12-11T19:04:04Z |
| publishDate | 2019-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | JOR Spine |
| spelling | doaj.art-237b15fc6a664706be885c0cc4f710ed2022-12-22T00:53:56ZengWileyJOR Spine2572-11432019-06-0122n/an/a10.1002/jsp2.1050Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury modelTadashi Nukaga0Daisuke Sakai1Jordy Schol2Masato Sato3Masahiko Watanabe4Department of Orthopaedic Surgery Tokai University School of Medicine Isehara Kanagawa JapanDepartment of Orthopaedic Surgery Tokai University School of Medicine Isehara Kanagawa JapanDepartment of Orthopaedic Surgery Tokai University School of Medicine Isehara Kanagawa JapanDepartment of Orthopaedic Surgery Tokai University School of Medicine Isehara Kanagawa JapanDepartment of Orthopaedic Surgery Tokai University School of Medicine Isehara Kanagawa JapanAbstract In recent years, studies have explored novel approaches for cell transplantation to enable annulus fibrosus (AF) regeneration of the intervertebral disc in particular for lumbar disc herniation. Nevertheless, successful engraftment of cells is structurally challenging, and no definitive method has yet been established. This study investigated the potential of cell sheet technology to facilitate cell engraftment for AF repair. AF injury was induced by a 1 × 1 mm defect in rat tails after which AF cell sheets were transplanted. Its regenerative effects were compared to a nondegenerated and degeneration only conditions. Degenerative changes of the entire intervertebral disc were examined by disc height measurements, histology, and immunohistochemistry for 4‐, 8‐, and 12‐weeks post‐transplantation. Cell engraftment was confirmed by tracing PKH26 fluorescent dyed AF cells. In the transplant group, disc degeneration was significantly suppressed after 4, 8, and 12 weeks when compared with the degenerative group, as indicated by histological scoring and DHI observations. At 2 and 4 weeks after transplant, PKH26 positive cells could be detected in defect region and surrounding AF. The results suggest cell engraftment into AF tissue could be established by the cell sheet technology without additional scaffolding or adhesives. In short, AF cell sheets appear to be an effective and accessible tool for AF repair and to support intervertebral disc regeneration.https://doi.org/10.1002/jsp2.1050annulus fibrosiscell sheetcell transplantationdisc herniationintervertebral discrat |
| spellingShingle | Tadashi Nukaga Daisuke Sakai Jordy Schol Masato Sato Masahiko Watanabe Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model JOR Spine annulus fibrosis cell sheet cell transplantation disc herniation intervertebral disc rat |
| title | Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model |
| title_full | Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model |
| title_fullStr | Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model |
| title_full_unstemmed | Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model |
| title_short | Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model |
| title_sort | annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model |
| topic | annulus fibrosis cell sheet cell transplantation disc herniation intervertebral disc rat |
| url | https://doi.org/10.1002/jsp2.1050 |
| work_keys_str_mv | AT tadashinukaga annulusfibrosuscellsheetslimitdiscdegenerationinaratannulusfibrosusinjurymodel AT daisukesakai annulusfibrosuscellsheetslimitdiscdegenerationinaratannulusfibrosusinjurymodel AT jordyschol annulusfibrosuscellsheetslimitdiscdegenerationinaratannulusfibrosusinjurymodel AT masatosato annulusfibrosuscellsheetslimitdiscdegenerationinaratannulusfibrosusinjurymodel AT masahikowatanabe annulusfibrosuscellsheetslimitdiscdegenerationinaratannulusfibrosusinjurymodel |