Genetic characterization and evolutionary analysis of emerging newcastle disease virus isolated from tibetan chickens
Newcastle disease (ND) is one of the highly contagious disease responsible for devastating outbreaks and considerable economic losses to poultry industry in China. However, no information is available about NDV in Tibet region; the aim of this study was to determine the genetic characterization and...
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| Format: | Article |
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Kafkas University, Faculty of Veterinary Medicine
2017-05-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2057 |
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| author | ZHU H ZHANG H WANG Y CIREN D DONG H WU Q REHMAN MU NABI F MEHMOOD K XU J LI J |
| author_facet | ZHU H ZHANG H WANG Y CIREN D DONG H WU Q REHMAN MU NABI F MEHMOOD K XU J LI J |
| author_sort | ZHU H |
| collection | DOAJ |
| description | Newcastle disease (ND) is one of the highly contagious disease responsible for devastating outbreaks and considerable economic losses to poultry
industry in China. However, no information is available about NDV in Tibet region; the aim of this study was to determine the genetic characterization
and evolutionary analysis of NDV in Tibetan chickens. Four NDVs were isolated from an outbreak in Tibetan chickens. The pathogenicity of the isolates was
determined by mean death time (MDT), intracerebral pathogenicity index (ICPI) and amino acid sequencing (112 to 117) of F protein. While the genetic
characterization of F gene was determined by sequencing the isolated NDVs and phylogenetic relationship was established with the help of reference
strains. Pathogenicity experiments revealed that XZ-F10 and XZ-F20 strains were lentogenic pathotype, while the remaining XZ-F2-1 and XZ-F4-6 strains
were velogenic pathotype. The deduced amino acid sequences of the cleavage site of the F protein confirmed our results. Phylogenic analysis of these
strains indicated that both XZ-F2-1 and XZ-F4-6 belong to genotype VII. However, XZ-F10 and XZ-F20 strains were assigned to genotypes II. The present
study highlights the need for continuous surveillance of NDV in Tibetan chickens; moreover, this study will provide a reference for the local government to
make the strategies and control emerging NDV in Tibet. |
| first_indexed | 2024-03-13T04:37:02Z |
| format | Article |
| id | doaj.art-237c6caec3c54e6382240a651f19614e |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:02Z |
| publishDate | 2017-05-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-237c6caec3c54e6382240a651f19614e2023-06-19T06:45:54ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512017-05-0123338538910.9775/kvfd.2016.167532057Genetic characterization and evolutionary analysis of emerging newcastle disease virus isolated from tibetan chickensZHU H0ZHANG H1WANG Y2CIREN D3DONG H4WU Q5REHMAN MU6NABI F7MEHMOOD K8XU J9LI J10Key Laboratory of Clinical Veterinary Medicine in Tibet, XiZang Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, PEOPLE’S REPUBLIC of CHINACollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PEOPLE’S REPUBLIC of CHINACollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PEOPLE’S REPUBLIC of CHINAKey Laboratory of Clinical Veterinary Medicine in Tibet, XiZang Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, PEOPLE’S REPUBLIC of CHINAKey Laboratory of Clinical Veterinary Medicine in Tibet, XiZang Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, PEOPLE’S REPUBLIC of CHINAKey Laboratory of Clinical Veterinary Medicine in Tibet, XiZang Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, PEOPLE’S REPUBLIC of CHINACollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PEOPLE’S REPUBLIC of CHINACollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PEOPLE’S REPUBLIC of CHINACollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PEOPLE’S REPUBLIC of CHINACollege of Animal Science, Anhui Science and Technology University, Fengyang, 233100, PEOPLE’S REPUBLIC of CHINAKey Laboratory of Clinical Veterinary Medicine in Tibet, XiZang Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, PEOPLE’S REPUBLIC of CHINANewcastle disease (ND) is one of the highly contagious disease responsible for devastating outbreaks and considerable economic losses to poultry industry in China. However, no information is available about NDV in Tibet region; the aim of this study was to determine the genetic characterization and evolutionary analysis of NDV in Tibetan chickens. Four NDVs were isolated from an outbreak in Tibetan chickens. The pathogenicity of the isolates was determined by mean death time (MDT), intracerebral pathogenicity index (ICPI) and amino acid sequencing (112 to 117) of F protein. While the genetic characterization of F gene was determined by sequencing the isolated NDVs and phylogenetic relationship was established with the help of reference strains. Pathogenicity experiments revealed that XZ-F10 and XZ-F20 strains were lentogenic pathotype, while the remaining XZ-F2-1 and XZ-F4-6 strains were velogenic pathotype. The deduced amino acid sequences of the cleavage site of the F protein confirmed our results. Phylogenic analysis of these strains indicated that both XZ-F2-1 and XZ-F4-6 belong to genotype VII. However, XZ-F10 and XZ-F20 strains were assigned to genotypes II. The present study highlights the need for continuous surveillance of NDV in Tibetan chickens; moreover, this study will provide a reference for the local government to make the strategies and control emerging NDV in Tibet.https://vetdergikafkas.org/pdf.php?id=2057newcastle disease viruses (ndvs)fusion protein (f) genemolecular characterizationpathogenicitytibetan chickens |
| spellingShingle | ZHU H ZHANG H WANG Y CIREN D DONG H WU Q REHMAN MU NABI F MEHMOOD K XU J LI J Genetic characterization and evolutionary analysis of emerging newcastle disease virus isolated from tibetan chickens Kafkas Universitesi Veteriner Fakültesi Dergisi newcastle disease viruses (ndvs) fusion protein (f) gene molecular characterization pathogenicity tibetan chickens |
| title | Genetic characterization and evolutionary analysis of emerging newcastle disease virus isolated from tibetan chickens |
| title_full | Genetic characterization and evolutionary analysis of emerging newcastle disease virus isolated from tibetan chickens |
| title_fullStr | Genetic characterization and evolutionary analysis of emerging newcastle disease virus isolated from tibetan chickens |
| title_full_unstemmed | Genetic characterization and evolutionary analysis of emerging newcastle disease virus isolated from tibetan chickens |
| title_short | Genetic characterization and evolutionary analysis of emerging newcastle disease virus isolated from tibetan chickens |
| title_sort | genetic characterization and evolutionary analysis of emerging newcastle disease virus isolated from tibetan chickens |
| topic | newcastle disease viruses (ndvs) fusion protein (f) gene molecular characterization pathogenicity tibetan chickens |
| url | https://vetdergikafkas.org/pdf.php?id=2057 |
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