Prospects in Cadmium-Contaminated Water Management Using Free-Living Cyanobacteria (<i>Oscillatoria</i> sp.)
In this study, the removal of cadmium (Cd) by free-living <i>Oscillatoria</i> sp. was studied. Our results showed that maximal Cd removal efficiency (~60%) by the cyanobacterial culture was achieved within 12–24 h in the presence of 5.0 or 25.0 mg/L of Cd. The mechanisms underlying this...
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MDPI AG
2021-02-01
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| Series: | Water |
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| Online Access: | https://www.mdpi.com/2073-4441/13/4/542 |
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| author | Iván Carralero Bon Lucas M. Salvatierra Luciana D. Lario Jordi Morató Leonardo M. Pérez |
| author_facet | Iván Carralero Bon Lucas M. Salvatierra Luciana D. Lario Jordi Morató Leonardo M. Pérez |
| author_sort | Iván Carralero Bon |
| collection | DOAJ |
| description | In this study, the removal of cadmium (Cd) by free-living <i>Oscillatoria</i> sp. was studied. Our results showed that maximal Cd removal efficiency (~60%) by the cyanobacterial culture was achieved within 12–24 h in the presence of 5.0 or 25.0 mg/L of Cd. The mechanisms underlying this phenomenon were explored by elemental analysis and FTIR-ATR spectroscopy. It was found that metal adsorption by negatively charged functional groups in the cyanobacterial biomass was the main mechanism used by <i>Oscillatoria</i> sp. to remove metal from the aqueous medium, followed by Cd bioaccumulation into living cells. Additionally, Cd-exposed microalgae showed increased oxidative stress (MDA formation), a decreased dehydrogenase activity, a higher amount of soluble carbohydrates and a decreased total carotenoid concentration, as compared to the control cells. These results suggest that <i>Oscillatoria</i> sp. improved its antioxidative defense system under stressful conditions, through carotenoid-mediated ROS quenching and induction of carbohydrate catabolism, in order to counteract the oxidative damage and preserve the photosynthetic machinery and cellular energetics. In fact, no significant reduction in <i>Oscillatoria</i> sp. cell density, total protein amount, and chlorophyll a content was observed after 24-h Cd exposure, even at the highest metal concentration tested (i.e., 25.0 mg/L). Hence, the presented results are the first to describe some new insights about the metabolic and physiological behavior of living <i>Oscillatoria</i> sp. during Cd remediation, and open up the possibility of finding an equilibrium that maximizes metal removal performance with an active cyanobacterial metabolism, to achieve a rewarding and sustainable management of industrial metal-polluted wastewater. |
| first_indexed | 2024-03-09T00:41:23Z |
| format | Article |
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| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-09T00:41:23Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-237e1a0f04e44c6cb7c0d1297dcaa1c22023-12-11T17:45:29ZengMDPI AGWater2073-44412021-02-0113454210.3390/w13040542Prospects in Cadmium-Contaminated Water Management Using Free-Living Cyanobacteria (<i>Oscillatoria</i> sp.)Iván Carralero Bon0Lucas M. Salvatierra1Luciana D. Lario2Jordi Morató3Leonardo M. Pérez4Grupo de Biotecnología de Materiales y Medioambiente (Bio&TecMA), Instituto de Investigaciones en Ingeniería Ambiental, Química y Biotecnología Aplicada (INGEBIO-UCA), Facultad de Química e Ingeniería del Rosario, Pontificia Universidad Católica Argentina (UCA), Av. Pellegrini 3314, Rosario S2002QEO, ArgentinaGrupo de Biotecnología de Materiales y Medioambiente (Bio&TecMA), Instituto de Investigaciones en Ingeniería Ambiental, Química y Biotecnología Aplicada (INGEBIO-UCA), Facultad de Química e Ingeniería del Rosario, Pontificia Universidad Católica Argentina (UCA), Av. Pellegrini 3314, Rosario S2002QEO, ArgentinaGrupo de Biotecnología de Materiales y Medioambiente (Bio&TecMA), Instituto de Investigaciones en Ingeniería Ambiental, Química y Biotecnología Aplicada (INGEBIO-UCA), Facultad de Química e Ingeniería del Rosario, Pontificia Universidad Católica Argentina (UCA), Av. Pellegrini 3314, Rosario S2002QEO, ArgentinaUNESCO Chair on Sustainability, Universitat Politècnica de Catalunya-BarcelonaTech, C/Colom, 1, TR1, ESEIAAT, 08222 Terrassa, SpainGrupo de Biotecnología de Materiales y Medioambiente (Bio&TecMA), Instituto de Investigaciones en Ingeniería Ambiental, Química y Biotecnología Aplicada (INGEBIO-UCA), Facultad de Química e Ingeniería del Rosario, Pontificia Universidad Católica Argentina (UCA), Av. Pellegrini 3314, Rosario S2002QEO, ArgentinaIn this study, the removal of cadmium (Cd) by free-living <i>Oscillatoria</i> sp. was studied. Our results showed that maximal Cd removal efficiency (~60%) by the cyanobacterial culture was achieved within 12–24 h in the presence of 5.0 or 25.0 mg/L of Cd. The mechanisms underlying this phenomenon were explored by elemental analysis and FTIR-ATR spectroscopy. It was found that metal adsorption by negatively charged functional groups in the cyanobacterial biomass was the main mechanism used by <i>Oscillatoria</i> sp. to remove metal from the aqueous medium, followed by Cd bioaccumulation into living cells. Additionally, Cd-exposed microalgae showed increased oxidative stress (MDA formation), a decreased dehydrogenase activity, a higher amount of soluble carbohydrates and a decreased total carotenoid concentration, as compared to the control cells. These results suggest that <i>Oscillatoria</i> sp. improved its antioxidative defense system under stressful conditions, through carotenoid-mediated ROS quenching and induction of carbohydrate catabolism, in order to counteract the oxidative damage and preserve the photosynthetic machinery and cellular energetics. In fact, no significant reduction in <i>Oscillatoria</i> sp. cell density, total protein amount, and chlorophyll a content was observed after 24-h Cd exposure, even at the highest metal concentration tested (i.e., 25.0 mg/L). Hence, the presented results are the first to describe some new insights about the metabolic and physiological behavior of living <i>Oscillatoria</i> sp. during Cd remediation, and open up the possibility of finding an equilibrium that maximizes metal removal performance with an active cyanobacterial metabolism, to achieve a rewarding and sustainable management of industrial metal-polluted wastewater.https://www.mdpi.com/2073-4441/13/4/542cadmium-polluted waterecotechnologiesbioremediationfree-living cyanobacteria<i>Oscillatoria</i> sp. |
| spellingShingle | Iván Carralero Bon Lucas M. Salvatierra Luciana D. Lario Jordi Morató Leonardo M. Pérez Prospects in Cadmium-Contaminated Water Management Using Free-Living Cyanobacteria (<i>Oscillatoria</i> sp.) Water cadmium-polluted water ecotechnologies bioremediation free-living cyanobacteria <i>Oscillatoria</i> sp. |
| title | Prospects in Cadmium-Contaminated Water Management Using Free-Living Cyanobacteria (<i>Oscillatoria</i> sp.) |
| title_full | Prospects in Cadmium-Contaminated Water Management Using Free-Living Cyanobacteria (<i>Oscillatoria</i> sp.) |
| title_fullStr | Prospects in Cadmium-Contaminated Water Management Using Free-Living Cyanobacteria (<i>Oscillatoria</i> sp.) |
| title_full_unstemmed | Prospects in Cadmium-Contaminated Water Management Using Free-Living Cyanobacteria (<i>Oscillatoria</i> sp.) |
| title_short | Prospects in Cadmium-Contaminated Water Management Using Free-Living Cyanobacteria (<i>Oscillatoria</i> sp.) |
| title_sort | prospects in cadmium contaminated water management using free living cyanobacteria i oscillatoria i sp |
| topic | cadmium-polluted water ecotechnologies bioremediation free-living cyanobacteria <i>Oscillatoria</i> sp. |
| url | https://www.mdpi.com/2073-4441/13/4/542 |
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