Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological Protection
Pomegranate extract (PG-E) has been reported to exert a protective effect on the skin due to its antioxidant activity. Ingredients rich in phenolic compounds are unstable in extract solutions, and, therefore, the use of a suitable nanosystem to encapsulate this type of extract could be necessary in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/2/271 |
| _version_ | 1797396165914787840 |
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| author | Lucía Yepes-Molina José A. Hernández Micaela Carvajal |
| author_facet | Lucía Yepes-Molina José A. Hernández Micaela Carvajal |
| author_sort | Lucía Yepes-Molina |
| collection | DOAJ |
| description | Pomegranate extract (PG-E) has been reported to exert a protective effect on the skin due to its antioxidant activity. Ingredients rich in phenolic compounds are unstable in extract solutions, and, therefore, the use of a suitable nanosystem to encapsulate this type of extract could be necessary in different biotechnological applications. Thus, we investigated the capacity of <i>Brassica oleracea</i> L. (cauliflower) inflorescence vesicles (CI-vesicles) to encapsulate PG-E and determined the stability and the antioxidant capacity of the system over time. In addition, the protective effect against UV radiation and heavy metals in HaCaT cells was also tested. The CI-vesicles had an entrapment efficiency of around 50%, and accelerated stability tests did not show significant changes in the parameters tested. The results for the HaCaT cells showed the non-cytotoxicity of the CI-vesicles containing PG-E and their protection against heavy metals (lead acetate and mercuric chloride) and UV-B radiation through a reduction of oxidative stress. The reduction of the percentage of deleted mtDNA (mtDNA4977, “common deletion”) in UV-treated HaCaT cells due to the presence of CI-vesicles containing PG-E indicated the mechanism of protection. Therefore, the effects of CI-vesicles loaded with PG-E against oxidative stress support their utilization as natural cosmeceuticals to protect skin health against external damage from environmental pollution and UV radiation. |
| first_indexed | 2024-03-09T00:47:21Z |
| format | Article |
| id | doaj.art-237ebd24767c4c1abf7fea10e7d5b54e |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T00:47:21Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-237ebd24767c4c1abf7fea10e7d5b54e2023-12-11T17:25:53ZengMDPI AGPharmaceutics1999-49232021-02-0113227110.3390/pharmaceutics13020271Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological ProtectionLucía Yepes-Molina0José A. Hernández1Micaela Carvajal2Aquaporins Group, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus de Espinardo, E-30100 Murcia, SpainBiotechnology of Fruit Trees Group, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus de Espinardo, E-30100 Murcia, SpainAquaporins Group, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus de Espinardo, E-30100 Murcia, SpainPomegranate extract (PG-E) has been reported to exert a protective effect on the skin due to its antioxidant activity. Ingredients rich in phenolic compounds are unstable in extract solutions, and, therefore, the use of a suitable nanosystem to encapsulate this type of extract could be necessary in different biotechnological applications. Thus, we investigated the capacity of <i>Brassica oleracea</i> L. (cauliflower) inflorescence vesicles (CI-vesicles) to encapsulate PG-E and determined the stability and the antioxidant capacity of the system over time. In addition, the protective effect against UV radiation and heavy metals in HaCaT cells was also tested. The CI-vesicles had an entrapment efficiency of around 50%, and accelerated stability tests did not show significant changes in the parameters tested. The results for the HaCaT cells showed the non-cytotoxicity of the CI-vesicles containing PG-E and their protection against heavy metals (lead acetate and mercuric chloride) and UV-B radiation through a reduction of oxidative stress. The reduction of the percentage of deleted mtDNA (mtDNA4977, “common deletion”) in UV-treated HaCaT cells due to the presence of CI-vesicles containing PG-E indicated the mechanism of protection. Therefore, the effects of CI-vesicles loaded with PG-E against oxidative stress support their utilization as natural cosmeceuticals to protect skin health against external damage from environmental pollution and UV radiation.https://www.mdpi.com/1999-4923/13/2/271pomegranateantioxidant capacitymembrane vesiclescauliflowerkeratinocytesoxidative stress |
| spellingShingle | Lucía Yepes-Molina José A. Hernández Micaela Carvajal Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological Protection Pharmaceutics pomegranate antioxidant capacity membrane vesicles cauliflower keratinocytes oxidative stress |
| title | Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological Protection |
| title_full | Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological Protection |
| title_fullStr | Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological Protection |
| title_full_unstemmed | Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological Protection |
| title_short | Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological Protection |
| title_sort | nanoencapsulation of pomegranate extract to increase stability and potential dermatological protection |
| topic | pomegranate antioxidant capacity membrane vesicles cauliflower keratinocytes oxidative stress |
| url | https://www.mdpi.com/1999-4923/13/2/271 |
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