Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen
Objectives: This study was designed to examine the effects of various concentrations of tris (hydroxymethyl) aminomethane (tris) and egg yolk on the quality of cryopreserved buck sperm. Materials and Methods: The collected semen samples were pooled, washed, and diluted into five different freezing...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2022-12-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=83945 |
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| author | Md. Mostofa Kamal Md. Emtiaj Alam Md. Akhtarul Islam Md. Royhan Gofur Aurangazeb Kabir |
| author_facet | Md. Mostofa Kamal Md. Emtiaj Alam Md. Akhtarul Islam Md. Royhan Gofur Aurangazeb Kabir |
| author_sort | Md. Mostofa Kamal |
| collection | DOAJ |
| description | Objectives: This study was designed to examine the effects of various concentrations of tris (hydroxymethyl) aminomethane (tris) and egg yolk on the quality of cryopreserved buck sperm.
Materials and Methods: The collected semen samples were pooled, washed, and diluted into five different freezing extender groups, viz., extender I (tris 0% + egg yolk 0%), extender II (tris 1.41% + egg yolk 4%), extender III (tris 2.41% + egg yolk 8%), extender IV (tris 3.41% + egg yolk 16%), and extender V (tris 4.41% + egg yolk 24%). The sperm parameter of the five groups of extenders was evaluated after equilibration and cryopreservation.
Results: The results showed that extenders II–V provided significantly higher semen progressive motility and total motility percentages than extender I after equilibration (p < 0.05). The higher percentages of semen progressive motility, total motility, viability, and plasma membrane integ¬rity (by both HOST under light microscopy and stain after HOST under light microscopy) were found in the sperm cryopreserved with extender IV than extender I, extender II, and extender III groups after thawing (p < 0.05). In addition, semen progressive motility, total motility, and viability were not further increased, or plasma membrane integrity (by both HOST tests) was decreased by the addition of tris and egg yolk (extender V) after cryopreservation (p < 0.05).
Conclusion: In conclusion, our result indicates that the following washing, the supplementation of tris (3.41% + egg yolk 16%) on the freezing extender are suitable for improving the semen quality of buck after freezing and thawing. [J Adv Vet Anim Res 2022; 9(4.000): 676-683] |
| first_indexed | 2024-04-11T00:00:49Z |
| format | Article |
| id | doaj.art-23807c1621a543528c47ccd17a424cb1 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-04-11T00:00:49Z |
| publishDate | 2022-12-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-23807c1621a543528c47ccd17a424cb12023-01-10T04:13:29ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102022-12-019467668310.5455/javar.2022.i63683945Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semenMd. Mostofa Kamal0Md. Emtiaj Alam1Md. Akhtarul Islam2Md. Royhan Gofur3Aurangazeb Kabir4Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh.Objectives: This study was designed to examine the effects of various concentrations of tris (hydroxymethyl) aminomethane (tris) and egg yolk on the quality of cryopreserved buck sperm. Materials and Methods: The collected semen samples were pooled, washed, and diluted into five different freezing extender groups, viz., extender I (tris 0% + egg yolk 0%), extender II (tris 1.41% + egg yolk 4%), extender III (tris 2.41% + egg yolk 8%), extender IV (tris 3.41% + egg yolk 16%), and extender V (tris 4.41% + egg yolk 24%). The sperm parameter of the five groups of extenders was evaluated after equilibration and cryopreservation. Results: The results showed that extenders II–V provided significantly higher semen progressive motility and total motility percentages than extender I after equilibration (p < 0.05). The higher percentages of semen progressive motility, total motility, viability, and plasma membrane integ¬rity (by both HOST under light microscopy and stain after HOST under light microscopy) were found in the sperm cryopreserved with extender IV than extender I, extender II, and extender III groups after thawing (p < 0.05). In addition, semen progressive motility, total motility, and viability were not further increased, or plasma membrane integrity (by both HOST tests) was decreased by the addition of tris and egg yolk (extender V) after cryopreservation (p < 0.05). Conclusion: In conclusion, our result indicates that the following washing, the supplementation of tris (3.41% + egg yolk 16%) on the freezing extender are suitable for improving the semen quality of buck after freezing and thawing. [J Adv Vet Anim Res 2022; 9(4.000): 676-683]http://www.ejmanager.com/fulltextpdf.php?mno=83945sperm; tris; egg yolk; freezing; thawing; semen quality |
| spellingShingle | Md. Mostofa Kamal Md. Emtiaj Alam Md. Akhtarul Islam Md. Royhan Gofur Aurangazeb Kabir Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen Journal of Advanced Veterinary and Animal Research sperm; tris; egg yolk; freezing; thawing; semen quality |
| title | Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen |
| title_full | Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen |
| title_fullStr | Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen |
| title_full_unstemmed | Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen |
| title_short | Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen |
| title_sort | effects of tris hydroxymethyl aminomethane and egg yolk on the cryopreservation of buck semen |
| topic | sperm; tris; egg yolk; freezing; thawing; semen quality |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=83945 |
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