Exploring traditional aus-type rice for metabolites conferring drought tolerance
Abstract Background Traditional varieties and landraces belonging to the aus-type group of rice (Oryza sativa L.) are known to be highly tolerant to environmental stresses, such as drought and heat, and are therefore recognized as a valuable genetic resource for crop improvement. Using two aus-type...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-01-01
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| Series: | Rice |
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| Online Access: | http://link.springer.com/article/10.1186/s12284-017-0189-7 |
| _version_ | 1819072788117323776 |
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| author | Alberto Casartelli David Riewe Hans Michael Hubberten Thomas Altmann Rainer Hoefgen Sigrid Heuer |
| author_facet | Alberto Casartelli David Riewe Hans Michael Hubberten Thomas Altmann Rainer Hoefgen Sigrid Heuer |
| author_sort | Alberto Casartelli |
| collection | DOAJ |
| description | Abstract Background Traditional varieties and landraces belonging to the aus-type group of rice (Oryza sativa L.) are known to be highly tolerant to environmental stresses, such as drought and heat, and are therefore recognized as a valuable genetic resource for crop improvement. Using two aus-type (Dular, N22) and two drought intolerant irrigated varieties (IR64, IR74) an untargeted metabolomics analysis was conducted to identify drought-responsive metabolites associated with tolerance. Results The superior drought tolerance of Dular and N22 compared with the irrigated varieties was confirmed by phenotyping plants grown to maturity after imposing severe drought stress in a dry-down treatment. Dular and N22 did not show a significant reduction in grain yield compared to well-watered control plants, whereas the intolerant varieties showed a significant reduction in both, total spikelet number and grain yield. The metabolomics analysis was conducted with shoot and root samples of plants at the tillering stage at the end of the dry-down treatment. The data revealed an overall higher accumulation of N-rich metabolites (amino acids and nucleotide-related metabolites allantoin and uridine) in shoots of the tolerant varieties. In roots, the aus-type varieties were characterised by a higher reduction of metabolites representative of glycolysis and the TCA cycle, such as malate, glyceric acid and glyceric acid-3-phosphate. On the other hand, the oligosaccharide raffinose showed a higher fold increase in both, shoots and roots of the sensitive genotypes. The data further showed that, for certain drought-responsive metabolites, differences between the contrasting rice varieties were already evident under well-watered control conditions. Conclusions The drought tolerance-related metabolites identified in the aus-type varieties provide a valuable set of protective compounds and an entry point for assessing genetic diversity in the underlying pathways for developing drought tolerant rice and other crops. |
| first_indexed | 2024-12-21T17:43:17Z |
| format | Article |
| id | doaj.art-23815d4f431e46a4a50a72595975ab0a |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-21T17:43:17Z |
| publishDate | 2018-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-23815d4f431e46a4a50a72595975ab0a2022-12-21T18:55:34ZengSpringerOpenRice1939-84251939-84332018-01-0111111610.1186/s12284-017-0189-7Exploring traditional aus-type rice for metabolites conferring drought toleranceAlberto Casartelli0David Riewe1Hans Michael Hubberten2Thomas Altmann3Rainer Hoefgen4Sigrid Heuer5School of Agriculture, Food and Wine, Waite Campus, The University of AdelaideJulius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product ProtectionMax Planck Institute of Molecular Plant PhysiologyJulius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product ProtectionMax Planck Institute of Molecular Plant PhysiologySchool of Agriculture, Food and Wine, Waite Campus, The University of AdelaideAbstract Background Traditional varieties and landraces belonging to the aus-type group of rice (Oryza sativa L.) are known to be highly tolerant to environmental stresses, such as drought and heat, and are therefore recognized as a valuable genetic resource for crop improvement. Using two aus-type (Dular, N22) and two drought intolerant irrigated varieties (IR64, IR74) an untargeted metabolomics analysis was conducted to identify drought-responsive metabolites associated with tolerance. Results The superior drought tolerance of Dular and N22 compared with the irrigated varieties was confirmed by phenotyping plants grown to maturity after imposing severe drought stress in a dry-down treatment. Dular and N22 did not show a significant reduction in grain yield compared to well-watered control plants, whereas the intolerant varieties showed a significant reduction in both, total spikelet number and grain yield. The metabolomics analysis was conducted with shoot and root samples of plants at the tillering stage at the end of the dry-down treatment. The data revealed an overall higher accumulation of N-rich metabolites (amino acids and nucleotide-related metabolites allantoin and uridine) in shoots of the tolerant varieties. In roots, the aus-type varieties were characterised by a higher reduction of metabolites representative of glycolysis and the TCA cycle, such as malate, glyceric acid and glyceric acid-3-phosphate. On the other hand, the oligosaccharide raffinose showed a higher fold increase in both, shoots and roots of the sensitive genotypes. The data further showed that, for certain drought-responsive metabolites, differences between the contrasting rice varieties were already evident under well-watered control conditions. Conclusions The drought tolerance-related metabolites identified in the aus-type varieties provide a valuable set of protective compounds and an entry point for assessing genetic diversity in the underlying pathways for developing drought tolerant rice and other crops.http://link.springer.com/article/10.1186/s12284-017-0189-7RiceAus-type landracesMetabolitesAllantoinDrought toleranceRoots |
| spellingShingle | Alberto Casartelli David Riewe Hans Michael Hubberten Thomas Altmann Rainer Hoefgen Sigrid Heuer Exploring traditional aus-type rice for metabolites conferring drought tolerance Rice Rice Aus-type landraces Metabolites Allantoin Drought tolerance Roots |
| title | Exploring traditional aus-type rice for metabolites conferring drought tolerance |
| title_full | Exploring traditional aus-type rice for metabolites conferring drought tolerance |
| title_fullStr | Exploring traditional aus-type rice for metabolites conferring drought tolerance |
| title_full_unstemmed | Exploring traditional aus-type rice for metabolites conferring drought tolerance |
| title_short | Exploring traditional aus-type rice for metabolites conferring drought tolerance |
| title_sort | exploring traditional aus type rice for metabolites conferring drought tolerance |
| topic | Rice Aus-type landraces Metabolites Allantoin Drought tolerance Roots |
| url | http://link.springer.com/article/10.1186/s12284-017-0189-7 |
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