Electroacupuncture intervention of visceral hypersensitivity is involved in PAR-2-activation and CGRP-release in the spinal cord
Abstract Electroacupuncture (EA) relieves visceral hypersensitivity (VH) with underlying inflammatory bowel diseases. However, the mechanism by which EA treats ileitis-induced VH is not clearly known. To assess the effects of EA on ileitis-induced VH and confirm whether EA attenuates VH through spin...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-07-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-67702-2 |
| _version_ | 1819202687819841536 |
|---|---|
| author | Manoj K. Shah Yi Ding Juan Wan Habibullah Janyaro Adnan Hassan Tahir Vitaly Vodyanoy Ming-Xing Ding |
| author_facet | Manoj K. Shah Yi Ding Juan Wan Habibullah Janyaro Adnan Hassan Tahir Vitaly Vodyanoy Ming-Xing Ding |
| author_sort | Manoj K. Shah |
| collection | DOAJ |
| description | Abstract Electroacupuncture (EA) relieves visceral hypersensitivity (VH) with underlying inflammatory bowel diseases. However, the mechanism by which EA treats ileitis-induced VH is not clearly known. To assess the effects of EA on ileitis-induced VH and confirm whether EA attenuates VH through spinal PAR-2 activation and CGRP release, goats received an injection of 2,4,6-trinitro-benzenesulfonic-acid (TNBS) solution into the ileal wall. TNBS-injected goats were allocated into VH, Sham acupuncture (Sham-A) and EA groups, while goats treated with saline instead of TNBS solution were used as the control. Goats in EA group received EA at bilateral Hou-San-Li acupoints for 0.5 h at 7 days and thereafter repeated every 3 days for 6 times. Goats in the Sham-A group were inserted with needles for 0.5 h at the aforementioned acupoints without any hand manipulation and electric stimulation. Visceromotor responses to colorectal distension, an indicator of VH, were recorded by electromyography. The terminal ileum and thoracic spinal cord (T11) were sampled for evaluating ileitis at days 7 and 22, and distribution and expression-levels of PAR-2, CGRP and c-Fos on day 22. TNBS-treated-goats exhibited apparent transmural-ileitis on day 7, microscopically low-grade ileitis on day 22 and VH at days 7–22. Goats of Sham-A, VH or EA group showed higher (P < 0.01) VH at days 7–22 than the Control-goats. EA-treated goats exhibited lower (P < 0.01) VH as compared with Sham-A or VH group. Immunoreactive-cells and expression-levels of spinal PAR-2, CGRP and c-Fos in the EA group were greater (P < 0.01) than those in the Control group, but less (P < 0.01) than those in Sham-A and VH groups on day 22. Downregulation of spinal PAR-2 and CGRP levels by EA attenuates the ileitis and resultant VH. |
| first_indexed | 2024-12-23T04:07:59Z |
| format | Article |
| id | doaj.art-23832ded794c48e69b407bd9330f15fe |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T04:07:59Z |
| publishDate | 2020-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-23832ded794c48e69b407bd9330f15fe2022-12-21T18:00:35ZengNature PortfolioScientific Reports2045-23222020-07-0110111510.1038/s41598-020-67702-2Electroacupuncture intervention of visceral hypersensitivity is involved in PAR-2-activation and CGRP-release in the spinal cordManoj K. Shah0Yi Ding1Juan Wan2Habibullah Janyaro3Adnan Hassan Tahir4Vitaly Vodyanoy5Ming-Xing Ding6College of Veterinary Medicine, Huazhong Agricultural UniversityCollege of Veterinary Medicine, Huazhong Agricultural UniversityCollege of Veterinary Medicine, Huazhong Agricultural UniversityCollege of Veterinary Medicine, Huazhong Agricultural UniversityCollege of Veterinary Medicine, Huazhong Agricultural UniversityDepartment of Anatomy, Physiology and Pharmacology, Auburn UniversityCollege of Veterinary Medicine, Huazhong Agricultural UniversityAbstract Electroacupuncture (EA) relieves visceral hypersensitivity (VH) with underlying inflammatory bowel diseases. However, the mechanism by which EA treats ileitis-induced VH is not clearly known. To assess the effects of EA on ileitis-induced VH and confirm whether EA attenuates VH through spinal PAR-2 activation and CGRP release, goats received an injection of 2,4,6-trinitro-benzenesulfonic-acid (TNBS) solution into the ileal wall. TNBS-injected goats were allocated into VH, Sham acupuncture (Sham-A) and EA groups, while goats treated with saline instead of TNBS solution were used as the control. Goats in EA group received EA at bilateral Hou-San-Li acupoints for 0.5 h at 7 days and thereafter repeated every 3 days for 6 times. Goats in the Sham-A group were inserted with needles for 0.5 h at the aforementioned acupoints without any hand manipulation and electric stimulation. Visceromotor responses to colorectal distension, an indicator of VH, were recorded by electromyography. The terminal ileum and thoracic spinal cord (T11) were sampled for evaluating ileitis at days 7 and 22, and distribution and expression-levels of PAR-2, CGRP and c-Fos on day 22. TNBS-treated-goats exhibited apparent transmural-ileitis on day 7, microscopically low-grade ileitis on day 22 and VH at days 7–22. Goats of Sham-A, VH or EA group showed higher (P < 0.01) VH at days 7–22 than the Control-goats. EA-treated goats exhibited lower (P < 0.01) VH as compared with Sham-A or VH group. Immunoreactive-cells and expression-levels of spinal PAR-2, CGRP and c-Fos in the EA group were greater (P < 0.01) than those in the Control group, but less (P < 0.01) than those in Sham-A and VH groups on day 22. Downregulation of spinal PAR-2 and CGRP levels by EA attenuates the ileitis and resultant VH.https://doi.org/10.1038/s41598-020-67702-2 |
| spellingShingle | Manoj K. Shah Yi Ding Juan Wan Habibullah Janyaro Adnan Hassan Tahir Vitaly Vodyanoy Ming-Xing Ding Electroacupuncture intervention of visceral hypersensitivity is involved in PAR-2-activation and CGRP-release in the spinal cord Scientific Reports |
| title | Electroacupuncture intervention of visceral hypersensitivity is involved in PAR-2-activation and CGRP-release in the spinal cord |
| title_full | Electroacupuncture intervention of visceral hypersensitivity is involved in PAR-2-activation and CGRP-release in the spinal cord |
| title_fullStr | Electroacupuncture intervention of visceral hypersensitivity is involved in PAR-2-activation and CGRP-release in the spinal cord |
| title_full_unstemmed | Electroacupuncture intervention of visceral hypersensitivity is involved in PAR-2-activation and CGRP-release in the spinal cord |
| title_short | Electroacupuncture intervention of visceral hypersensitivity is involved in PAR-2-activation and CGRP-release in the spinal cord |
| title_sort | electroacupuncture intervention of visceral hypersensitivity is involved in par 2 activation and cgrp release in the spinal cord |
| url | https://doi.org/10.1038/s41598-020-67702-2 |
| work_keys_str_mv | AT manojkshah electroacupunctureinterventionofvisceralhypersensitivityisinvolvedinpar2activationandcgrpreleaseinthespinalcord AT yiding electroacupunctureinterventionofvisceralhypersensitivityisinvolvedinpar2activationandcgrpreleaseinthespinalcord AT juanwan electroacupunctureinterventionofvisceralhypersensitivityisinvolvedinpar2activationandcgrpreleaseinthespinalcord AT habibullahjanyaro electroacupunctureinterventionofvisceralhypersensitivityisinvolvedinpar2activationandcgrpreleaseinthespinalcord AT adnanhassantahir electroacupunctureinterventionofvisceralhypersensitivityisinvolvedinpar2activationandcgrpreleaseinthespinalcord AT vitalyvodyanoy electroacupunctureinterventionofvisceralhypersensitivityisinvolvedinpar2activationandcgrpreleaseinthespinalcord AT mingxingding electroacupunctureinterventionofvisceralhypersensitivityisinvolvedinpar2activationandcgrpreleaseinthespinalcord |