In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract
Biosynthesis is a green method for the synthesis of silver nanoparticles (AgNPs). This study aimed to assess the antifungal activity of two silver nanoparticle solutions, synthesized using beech bark extract (BBE) and acetate and nitrate silver salts (AgNP Acetate BBE and AgNP Nitrate BBE), their in...
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MDPI AG
2021-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/10/2153 |
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| author | Anca Delia Mare Cristina Nicoleta Ciurea Adrian Man Mihai Mareș Felicia Toma Lavinia Berța Corneliu Tanase |
| author_facet | Anca Delia Mare Cristina Nicoleta Ciurea Adrian Man Mihai Mareș Felicia Toma Lavinia Berța Corneliu Tanase |
| author_sort | Anca Delia Mare |
| collection | DOAJ |
| description | Biosynthesis is a green method for the synthesis of silver nanoparticles (AgNPs). This study aimed to assess the antifungal activity of two silver nanoparticle solutions, synthesized using beech bark extract (BBE) and acetate and nitrate silver salts (AgNP Acetate BBE and AgNP Nitrate BBE), their influence on biofilm production, their potential synergistic effects with fluconazole, on different <i>Candida</i> spp., and their influence on virulence factors of <i>C</i>. <i>albicans</i> (germ tube production, gene expression for <i>ALS3</i>, <i>SAP2</i>, <i>HSP70</i>). Both the AgNP BBEs presented different minimum inhibitory concentrations for all the studied <i>Candida</i> spp., but biofilm production was inhibited only for <i>C</i>. <i>albicans</i> and <i>C</i>. <i>guilliermondii</i>. The growth rates of all the studied <i>Candida</i> spp. were inhibited in the presence of both AgNP BBEs, except for <i>C</i>. <i>auris</i>. Synergistic activity was observed for <i>C</i>. <i>parapsilosis</i> and <i>C</i>. <i>guilliermondii</i>, for different combinations of fluconazole with both the AgNP BBEs. The germ tube production of <i>C</i>. <i>albicans</i> was slightly inhibited by the AgNP BBEs. Only AgNP Acetate BBE was able to down-regulate the expression of <i>SAP2</i>. Overall, we can conclude that, even if more studies are necessary, AgNPs synthesized with beech bark extract might be an interesting alternative to classic antifungal treatments. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T06:15:01Z |
| publishDate | 2021-10-01 |
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| series | Plants |
| spelling | doaj.art-23848fc41b6349f09557653a3b6cae8c2023-11-22T19:44:41ZengMDPI AGPlants2223-77472021-10-011010215310.3390/plants10102153In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark ExtractAnca Delia Mare0Cristina Nicoleta Ciurea1Adrian Man2Mihai Mareș3Felicia Toma4Lavinia Berța5Corneliu Tanase6Department of Microbiology, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, 540139 Târgu Mureș, RomaniaDepartment of Microbiology, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, 540139 Târgu Mureș, RomaniaDepartment of Microbiology, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, 540139 Târgu Mureș, RomaniaLaboratory of Antimicrobial Chemotherapy, Ion Ionescu de la Brad University of Life Sciences, 8 Aleea Mihail Sadoveanu, 700489 Iași, RomaniaDepartment of Microbiology, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, 540139 Târgu Mureș, RomaniaDepartment of General and Inorganic Chemistry, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, 540139 Târgu Mureș, RomaniaDepartment of Pharmaceutical Botany, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, 540139 Târgu Mureș, RomaniaBiosynthesis is a green method for the synthesis of silver nanoparticles (AgNPs). This study aimed to assess the antifungal activity of two silver nanoparticle solutions, synthesized using beech bark extract (BBE) and acetate and nitrate silver salts (AgNP Acetate BBE and AgNP Nitrate BBE), their influence on biofilm production, their potential synergistic effects with fluconazole, on different <i>Candida</i> spp., and their influence on virulence factors of <i>C</i>. <i>albicans</i> (germ tube production, gene expression for <i>ALS3</i>, <i>SAP2</i>, <i>HSP70</i>). Both the AgNP BBEs presented different minimum inhibitory concentrations for all the studied <i>Candida</i> spp., but biofilm production was inhibited only for <i>C</i>. <i>albicans</i> and <i>C</i>. <i>guilliermondii</i>. The growth rates of all the studied <i>Candida</i> spp. were inhibited in the presence of both AgNP BBEs, except for <i>C</i>. <i>auris</i>. Synergistic activity was observed for <i>C</i>. <i>parapsilosis</i> and <i>C</i>. <i>guilliermondii</i>, for different combinations of fluconazole with both the AgNP BBEs. The germ tube production of <i>C</i>. <i>albicans</i> was slightly inhibited by the AgNP BBEs. Only AgNP Acetate BBE was able to down-regulate the expression of <i>SAP2</i>. Overall, we can conclude that, even if more studies are necessary, AgNPs synthesized with beech bark extract might be an interesting alternative to classic antifungal treatments.https://www.mdpi.com/2223-7747/10/10/2153silver nanoparticlesbeech barkantifungal activityfluconazole synergismbiofilm inhibition<i>ALS3</i> |
| spellingShingle | Anca Delia Mare Cristina Nicoleta Ciurea Adrian Man Mihai Mareș Felicia Toma Lavinia Berța Corneliu Tanase In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract Plants silver nanoparticles beech bark antifungal activity fluconazole synergism biofilm inhibition <i>ALS3</i> |
| title | In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract |
| title_full | In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract |
| title_fullStr | In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract |
| title_full_unstemmed | In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract |
| title_short | In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract |
| title_sort | in vitro antifungal activity of silver nanoparticles biosynthesized with beech bark extract |
| topic | silver nanoparticles beech bark antifungal activity fluconazole synergism biofilm inhibition <i>ALS3</i> |
| url | https://www.mdpi.com/2223-7747/10/10/2153 |
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