Local injection of adipose-derived mesenchymal stem cells in silk fibroin solution on the regeneration of lower esophageal sphincter in an animal model of GERD
Presently, various tissue engineering methods using adult stem cells and biomaterials are being confirmed to regenerate vessels, cardiac muscle, bladder, and intestines. However, there are few studies about the repair of the lower esophageal sphincter (LES) may help alleviate the symptoms of gastroe...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.993741/full |
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| author | Daxu Zhang Zhanbo Wang Lianjun Ma Lijuan Xu Suna Fan Yinan Su Xiaonan Shi Jingjing Hu Shuo Zhao WeiLong Li Enqiang Linghu Li Yan |
| author_facet | Daxu Zhang Zhanbo Wang Lianjun Ma Lijuan Xu Suna Fan Yinan Su Xiaonan Shi Jingjing Hu Shuo Zhao WeiLong Li Enqiang Linghu Li Yan |
| author_sort | Daxu Zhang |
| collection | DOAJ |
| description | Presently, various tissue engineering methods using adult stem cells and biomaterials are being confirmed to regenerate vessels, cardiac muscle, bladder, and intestines. However, there are few studies about the repair of the lower esophageal sphincter (LES) may help alleviate the symptoms of gastroesophageal reflux disease (GERD). This study aims to determine whether Adipose-Derived Stem Cells (ADSCs) combined with regenerated silk fibroin (RSF) solution could regenerate the LES. In vitro, the ADSCs were isolated, identified, and then cultured with an established smooth muscular induction system. In vivo, in the experimental groups, CM-Dil labeled ADSCs or induced ADSCs mixed with RSF solution were injected into the LES of rats after the development of the animal model of GERD respectively. The results showed that ADSCs could be induced into smooth muscular-like cells with the expression of h-caldesmon, calponin, α-smooth muscle actin, and a smooth muscle-myosin heavy chain in vitro. In vivo, the thickness of LES in the experiment rats was much thicker than those in the controlled groups. This result indicated that ADSCs mixed with RSF solution might contribute to the regeneration of the LES, thus reducing the occurrence of GERD. |
| first_indexed | 2024-04-09T20:00:31Z |
| format | Article |
| id | doaj.art-238823af78df42409c1cc4fbcad772fb |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-09T20:00:31Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-238823af78df42409c1cc4fbcad772fb2023-04-03T04:41:09ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-04-011110.3389/fcell.2023.993741993741Local injection of adipose-derived mesenchymal stem cells in silk fibroin solution on the regeneration of lower esophageal sphincter in an animal model of GERDDaxu Zhang0Zhanbo Wang1Lianjun Ma2Lijuan Xu3Suna Fan4Yinan Su5Xiaonan Shi6Jingjing Hu7Shuo Zhao8WeiLong Li9Enqiang Linghu10Li Yan11Department of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, ChinaDepartment of Pathology, Chinese PLA General Hospital, Beijing, ChinaEndoscopy Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Gastroenterology, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaState Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, ChinaDepartment of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, ChinaThe Second Medical Center and National Clinical Research Center of Geriatric Diseases, Chinese PLA General Hospital, Beijing, ChinaThe Second Medical Center and National Clinical Research Center of Geriatric Diseases, Chinese PLA General Hospital, Beijing, ChinaThe Second Medical Center and National Clinical Research Center of Geriatric Diseases, Chinese PLA General Hospital, Beijing, ChinaThe Second Medical Center and National Clinical Research Center of Geriatric Diseases, Chinese PLA General Hospital, Beijing, ChinaDepartment of Gastroenterology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaThe Second Medical Center and National Clinical Research Center of Geriatric Diseases, Chinese PLA General Hospital, Beijing, ChinaPresently, various tissue engineering methods using adult stem cells and biomaterials are being confirmed to regenerate vessels, cardiac muscle, bladder, and intestines. However, there are few studies about the repair of the lower esophageal sphincter (LES) may help alleviate the symptoms of gastroesophageal reflux disease (GERD). This study aims to determine whether Adipose-Derived Stem Cells (ADSCs) combined with regenerated silk fibroin (RSF) solution could regenerate the LES. In vitro, the ADSCs were isolated, identified, and then cultured with an established smooth muscular induction system. In vivo, in the experimental groups, CM-Dil labeled ADSCs or induced ADSCs mixed with RSF solution were injected into the LES of rats after the development of the animal model of GERD respectively. The results showed that ADSCs could be induced into smooth muscular-like cells with the expression of h-caldesmon, calponin, α-smooth muscle actin, and a smooth muscle-myosin heavy chain in vitro. In vivo, the thickness of LES in the experiment rats was much thicker than those in the controlled groups. This result indicated that ADSCs mixed with RSF solution might contribute to the regeneration of the LES, thus reducing the occurrence of GERD.https://www.frontiersin.org/articles/10.3389/fcell.2023.993741/fullADSCssmooth muscular cellsRSF solutiontransplantgerd |
| spellingShingle | Daxu Zhang Zhanbo Wang Lianjun Ma Lijuan Xu Suna Fan Yinan Su Xiaonan Shi Jingjing Hu Shuo Zhao WeiLong Li Enqiang Linghu Li Yan Local injection of adipose-derived mesenchymal stem cells in silk fibroin solution on the regeneration of lower esophageal sphincter in an animal model of GERD Frontiers in Cell and Developmental Biology ADSCs smooth muscular cells RSF solution transplant gerd |
| title | Local injection of adipose-derived mesenchymal stem cells in silk fibroin solution on the regeneration of lower esophageal sphincter in an animal model of GERD |
| title_full | Local injection of adipose-derived mesenchymal stem cells in silk fibroin solution on the regeneration of lower esophageal sphincter in an animal model of GERD |
| title_fullStr | Local injection of adipose-derived mesenchymal stem cells in silk fibroin solution on the regeneration of lower esophageal sphincter in an animal model of GERD |
| title_full_unstemmed | Local injection of adipose-derived mesenchymal stem cells in silk fibroin solution on the regeneration of lower esophageal sphincter in an animal model of GERD |
| title_short | Local injection of adipose-derived mesenchymal stem cells in silk fibroin solution on the regeneration of lower esophageal sphincter in an animal model of GERD |
| title_sort | local injection of adipose derived mesenchymal stem cells in silk fibroin solution on the regeneration of lower esophageal sphincter in an animal model of gerd |
| topic | ADSCs smooth muscular cells RSF solution transplant gerd |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.993741/full |
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