Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production
<i>Komagataeibacter</i> spp. has been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently, studies have demonstrated the capacity of <i>Komagataeibacter</i> spp. in the biotransformation of inhibitors found in...
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MDPI AG
2021-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2230 |
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| author | Pietro Cannazza Antti J. Rissanen Dieval Guizelini Pauli Losoi Essi Sarlin Diego Romano Ville Santala Rahul Mangayil |
| author_facet | Pietro Cannazza Antti J. Rissanen Dieval Guizelini Pauli Losoi Essi Sarlin Diego Romano Ville Santala Rahul Mangayil |
| author_sort | Pietro Cannazza |
| collection | DOAJ |
| description | <i>Komagataeibacter</i> spp. has been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently, studies have demonstrated the capacity of <i>Komagataeibacter</i> spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in <i>Komagataeibacter</i> spp. In this study, we isolated a cellulose-producing bacterium capable of synthesizing BC in a minimal medium containing crude glycerol, a by-product from the biodiesel production process. The isolate, affiliated to <i>Komagataeibacter</i> genus, synthesized cellulose in a minimal medium containing glucose (3.3 ± 0.3 g/L), pure glycerol (2.2 ± 0.1 g/L) and crude glycerol (2.1 ± 0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from the central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when <i>K. rhaeticus</i> ENS9b was grown in a minimal medium supplemented with acetate, BC production was not observed. However, in the presence of readily utilizable substrates, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis. |
| first_indexed | 2024-03-10T05:15:32Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:15:32Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-238a885180f84db1b2256af2b595ba0b2023-11-23T00:28:18ZengMDPI AGMicroorganisms2076-26072021-10-01911223010.3390/microorganisms9112230Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose ProductionPietro Cannazza0Antti J. Rissanen1Dieval Guizelini2Pauli Losoi3Essi Sarlin4Diego Romano5Ville Santala6Rahul Mangayil7Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133 Milan, ItalyFaculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, FinlandGraduate Program in Bioinformatics, Sector of Professional and Technological Education, Federal University of Parana (UFPR), Curitiba 81520-260, PR, BrazilFaculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, FinlandFaculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, FinlandDepartment of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133 Milan, ItalyFaculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, FinlandFaculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, Finland<i>Komagataeibacter</i> spp. has been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently, studies have demonstrated the capacity of <i>Komagataeibacter</i> spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in <i>Komagataeibacter</i> spp. In this study, we isolated a cellulose-producing bacterium capable of synthesizing BC in a minimal medium containing crude glycerol, a by-product from the biodiesel production process. The isolate, affiliated to <i>Komagataeibacter</i> genus, synthesized cellulose in a minimal medium containing glucose (3.3 ± 0.3 g/L), pure glycerol (2.2 ± 0.1 g/L) and crude glycerol (2.1 ± 0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from the central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when <i>K. rhaeticus</i> ENS9b was grown in a minimal medium supplemented with acetate, BC production was not observed. However, in the presence of readily utilizable substrates, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis.https://www.mdpi.com/2076-2607/9/11/2230<i>Komagataeibacter rhaeticus</i>bacterial cellulosecrude glycerolminimal mediumwhole-genome analysisacetate |
| spellingShingle | Pietro Cannazza Antti J. Rissanen Dieval Guizelini Pauli Losoi Essi Sarlin Diego Romano Ville Santala Rahul Mangayil Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production Microorganisms <i>Komagataeibacter rhaeticus</i> bacterial cellulose crude glycerol minimal medium whole-genome analysis acetate |
| title | Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production |
| title_full | Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production |
| title_fullStr | Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production |
| title_full_unstemmed | Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production |
| title_short | Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production |
| title_sort | characterization of i komagataeibacter i isolate reveals new prospects in waste stream valorization for bacterial cellulose production |
| topic | <i>Komagataeibacter rhaeticus</i> bacterial cellulose crude glycerol minimal medium whole-genome analysis acetate |
| url | https://www.mdpi.com/2076-2607/9/11/2230 |
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