Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain
Drosophila represents a key model organism for dissecting neuronal circuits that underlie innate and adaptive behavior. However, this task is limited by a lack of tools to monitor physiological parameters of spatially distributed, central synapses in identified neurons. We generated transgenic fly s...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-03-01
|
| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124715002429 |
| _version_ | 1818051379724288000 |
|---|---|
| author | Ulrike Pech Natalia H. Revelo Katharina J. Seitz Silvio O. Rizzoli André Fiala |
| author_facet | Ulrike Pech Natalia H. Revelo Katharina J. Seitz Silvio O. Rizzoli André Fiala |
| author_sort | Ulrike Pech |
| collection | DOAJ |
| description | Drosophila represents a key model organism for dissecting neuronal circuits that underlie innate and adaptive behavior. However, this task is limited by a lack of tools to monitor physiological parameters of spatially distributed, central synapses in identified neurons. We generated transgenic fly strains that express functional fluorescent reporters targeted to either pre- or postsynaptic compartments. Presynaptic Ca2+ dynamics are monitored using synaptophysin-coupled GCaMP3, synaptic transmission is monitored using red fluorescent synaptophysin-pHTomato, and postsynaptic Ca2+ dynamics are visualized using GCaMP3 fused with the postsynaptic matrix protein, dHomer. Using two-photon in vivo imaging of olfactory projection neurons, odor-evoked activity across populations of synapses is visualized in the antennal lobe and the mushroom body calyx. Prolonged odor exposure causes odor-specific and differential experience-dependent changes in pre- and postsynaptic activity at both levels of olfactory processing. The approach advances the physiological analysis of synaptic connections across defined groups of neurons in intact Drosophila. |
| first_indexed | 2024-12-10T11:08:26Z |
| format | Article |
| id | doaj.art-238d3d2027c84befb1dd1d26d085d929 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-10T11:08:26Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-238d3d2027c84befb1dd1d26d085d9292022-12-22T01:51:30ZengElsevierCell Reports2211-12472015-03-0110122083209510.1016/j.celrep.2015.02.065Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila BrainUlrike Pech0Natalia H. Revelo1Katharina J. Seitz2Silvio O. Rizzoli3André Fiala4Department of Molecular Neurobiology of Behavior, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University Göttingen, Julia-Lermontowa-Weg 3, 37077 Göttingen, GermanyDepartment of Neuro- and Sensory Physiology, University of Göttingen Medical Center, Humboldtallee 23, 37073 Göttingen, GermanyDepartment of Neuro- and Sensory Physiology, University of Göttingen Medical Center, Humboldtallee 23, 37073 Göttingen, GermanyDepartment of Neuro- and Sensory Physiology, University of Göttingen Medical Center, Humboldtallee 23, 37073 Göttingen, GermanyDepartment of Molecular Neurobiology of Behavior, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University Göttingen, Julia-Lermontowa-Weg 3, 37077 Göttingen, GermanyDrosophila represents a key model organism for dissecting neuronal circuits that underlie innate and adaptive behavior. However, this task is limited by a lack of tools to monitor physiological parameters of spatially distributed, central synapses in identified neurons. We generated transgenic fly strains that express functional fluorescent reporters targeted to either pre- or postsynaptic compartments. Presynaptic Ca2+ dynamics are monitored using synaptophysin-coupled GCaMP3, synaptic transmission is monitored using red fluorescent synaptophysin-pHTomato, and postsynaptic Ca2+ dynamics are visualized using GCaMP3 fused with the postsynaptic matrix protein, dHomer. Using two-photon in vivo imaging of olfactory projection neurons, odor-evoked activity across populations of synapses is visualized in the antennal lobe and the mushroom body calyx. Prolonged odor exposure causes odor-specific and differential experience-dependent changes in pre- and postsynaptic activity at both levels of olfactory processing. The approach advances the physiological analysis of synaptic connections across defined groups of neurons in intact Drosophila.http://www.sciencedirect.com/science/article/pii/S2211124715002429 |
| spellingShingle | Ulrike Pech Natalia H. Revelo Katharina J. Seitz Silvio O. Rizzoli André Fiala Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain Cell Reports |
| title | Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain |
| title_full | Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain |
| title_fullStr | Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain |
| title_full_unstemmed | Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain |
| title_short | Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain |
| title_sort | optical dissection of experience dependent pre and postsynaptic plasticity in the drosophila brain |
| url | http://www.sciencedirect.com/science/article/pii/S2211124715002429 |
| work_keys_str_mv | AT ulrikepech opticaldissectionofexperiencedependentpreandpostsynapticplasticityinthedrosophilabrain AT nataliahrevelo opticaldissectionofexperiencedependentpreandpostsynapticplasticityinthedrosophilabrain AT katharinajseitz opticaldissectionofexperiencedependentpreandpostsynapticplasticityinthedrosophilabrain AT silvioorizzoli opticaldissectionofexperiencedependentpreandpostsynapticplasticityinthedrosophilabrain AT andrefiala opticaldissectionofexperiencedependentpreandpostsynapticplasticityinthedrosophilabrain |