Conditioned Media From Glial Cells Promote a Neural-Like Connexin Expression in Human Adipose-Derived Mesenchymal Stem Cells
The expression of neuronal and glial connexins (Cxs) has been evaluated in adipose-derived mesenchymal stem cells (ASCs) whose neural differentiation was promoted by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). By immunocytochemistry...
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| Format: | Article |
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.01742/full |
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| author | Debora Lo Furno Giuliana Mannino Rosalia Pellitteri Agata Zappalà Rosalba Parenti Elisa Gili Carlo Vancheri Rosario Giuffrida |
| author_facet | Debora Lo Furno Giuliana Mannino Rosalia Pellitteri Agata Zappalà Rosalba Parenti Elisa Gili Carlo Vancheri Rosario Giuffrida |
| author_sort | Debora Lo Furno |
| collection | DOAJ |
| description | The expression of neuronal and glial connexins (Cxs) has been evaluated in adipose-derived mesenchymal stem cells (ASCs) whose neural differentiation was promoted by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). By immunocytochemistry and flow cytometer analysis it was found that Cx43 was already considerably expressed in naïve ASCs and further increased after 24 h and 7 days from CM exposition. Cx32 and Cx36 were significantly improved in conditioned cultures compared to control ASCs, whereas a decreased expression was noticed in the absence of CM treatments. Cx47 was virtually absent in any conditions. Altogether, high basal levels and induced increases of Cx43 expression suggest a potential attitude of ASCs toward an astrocyte differentiation, whereas the lack of Cx47 would indicate a poor propensity of ASCs to become oligodendrocytes. CM-evoked Cx32 and Cx36 increases showed that a neuronal- or a SC-like differentiation can be promoted by using this strategy. Results further confirm that environmental cues can favor an ASC neural differentiation, either as neuronal or glial elements. Of note, the use of glial products present in CM rather than the addition of chemical agents to achieve such differentiation would resemble “more physiological” conditions of differentiation. As a conclusion, the overexpression of typical neural Cxs would indicate the potential capability of neural-like ASCs to interact with neighboring neural cells and microenvironment. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-12T23:05:34Z |
| publishDate | 2018-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-2391c8f56a714f748831034202edb76b2022-12-22T03:12:55ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-11-01910.3389/fphys.2018.01742415007Conditioned Media From Glial Cells Promote a Neural-Like Connexin Expression in Human Adipose-Derived Mesenchymal Stem CellsDebora Lo Furno0Giuliana Mannino1Rosalia Pellitteri2Agata Zappalà3Rosalba Parenti4Elisa Gili5Carlo Vancheri6Rosario Giuffrida7Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, ItalyInstitute of Neurological Sciences, National Research Council, Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, Catania, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, ItalyThe expression of neuronal and glial connexins (Cxs) has been evaluated in adipose-derived mesenchymal stem cells (ASCs) whose neural differentiation was promoted by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). By immunocytochemistry and flow cytometer analysis it was found that Cx43 was already considerably expressed in naïve ASCs and further increased after 24 h and 7 days from CM exposition. Cx32 and Cx36 were significantly improved in conditioned cultures compared to control ASCs, whereas a decreased expression was noticed in the absence of CM treatments. Cx47 was virtually absent in any conditions. Altogether, high basal levels and induced increases of Cx43 expression suggest a potential attitude of ASCs toward an astrocyte differentiation, whereas the lack of Cx47 would indicate a poor propensity of ASCs to become oligodendrocytes. CM-evoked Cx32 and Cx36 increases showed that a neuronal- or a SC-like differentiation can be promoted by using this strategy. Results further confirm that environmental cues can favor an ASC neural differentiation, either as neuronal or glial elements. Of note, the use of glial products present in CM rather than the addition of chemical agents to achieve such differentiation would resemble “more physiological” conditions of differentiation. As a conclusion, the overexpression of typical neural Cxs would indicate the potential capability of neural-like ASCs to interact with neighboring neural cells and microenvironment.https://www.frontiersin.org/article/10.3389/fphys.2018.01742/fullhuman adipose mesenchymal stem cellsneural-like differentiationconnexin expressionglial conditioned mediaolfactory ensheathing cellsSchwann cells |
| spellingShingle | Debora Lo Furno Giuliana Mannino Rosalia Pellitteri Agata Zappalà Rosalba Parenti Elisa Gili Carlo Vancheri Rosario Giuffrida Conditioned Media From Glial Cells Promote a Neural-Like Connexin Expression in Human Adipose-Derived Mesenchymal Stem Cells Frontiers in Physiology human adipose mesenchymal stem cells neural-like differentiation connexin expression glial conditioned media olfactory ensheathing cells Schwann cells |
| title | Conditioned Media From Glial Cells Promote a Neural-Like Connexin Expression in Human Adipose-Derived Mesenchymal Stem Cells |
| title_full | Conditioned Media From Glial Cells Promote a Neural-Like Connexin Expression in Human Adipose-Derived Mesenchymal Stem Cells |
| title_fullStr | Conditioned Media From Glial Cells Promote a Neural-Like Connexin Expression in Human Adipose-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Conditioned Media From Glial Cells Promote a Neural-Like Connexin Expression in Human Adipose-Derived Mesenchymal Stem Cells |
| title_short | Conditioned Media From Glial Cells Promote a Neural-Like Connexin Expression in Human Adipose-Derived Mesenchymal Stem Cells |
| title_sort | conditioned media from glial cells promote a neural like connexin expression in human adipose derived mesenchymal stem cells |
| topic | human adipose mesenchymal stem cells neural-like differentiation connexin expression glial conditioned media olfactory ensheathing cells Schwann cells |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.01742/full |
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