Association polymorphism of guanine nucleotide–binding protein β3 subunit (GNB3) C825T and insertion/deletion of the angiotensin-converting enzyme (ACE) gene with peripartum cardiomyopathy
IntroductionPeripartum cardiomyopathy (PPCM) is a potentially life-threatening pregnancy-related heart disease. Genetic roles such as gene polymorphisms may relate to the etiology of PPCM. This study analyzes the association between single nucleotide gene polymorphism (SNP) guanine nucleotide–bindin...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1096514/full |
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| author | Ivana Purnama Dewi Ivana Purnama Dewi Louisa Fadjri Kusuma Wardhani Irma Maghfirah Kristin Purnama Dewi Kristin Purnama Dewi Agus Subagjo Mochamad Yusuf Alsagaff Johanes Nugroho |
| author_facet | Ivana Purnama Dewi Ivana Purnama Dewi Louisa Fadjri Kusuma Wardhani Irma Maghfirah Kristin Purnama Dewi Kristin Purnama Dewi Agus Subagjo Mochamad Yusuf Alsagaff Johanes Nugroho |
| author_sort | Ivana Purnama Dewi |
| collection | DOAJ |
| description | IntroductionPeripartum cardiomyopathy (PPCM) is a potentially life-threatening pregnancy-related heart disease. Genetic roles such as gene polymorphisms may relate to the etiology of PPCM. This study analyzes the association between single nucleotide gene polymorphism (SNP) guanine nucleotide–binding protein beta-3 subunit (GNB3) C825T and insertion/deletion (I/D) of the angiotensin-converting enzyme (ACE) gene with the incidence of PPCM.MethodsAn analytic observational study with a case–control design was conducted at the Integrated Cardiac Service Center of Dr. Soetomo General Hospital, Surabaya, Indonesia. PPCM patients of the case and control groups were enrolled. Baseline characteristic data were collected and blood samples were analyzed for SNP in the GNB3 C825T gene and for I/D in the ACE gene by using the polymerase chain reaction, restriction fragment length polymorphism, and Sanger sequencing. We also assessed ACE levels among different ACE genotypes using a sandwich-ELISA test.ResultsA total of 100 patients were included in this study, with 34 PPCM cases and 66 controls. There were significant differences in GNB3 TT and TC genotypes in the case group compared with that in the control group (TT: 35.3% vs. 10.6%, p = 0.003; TC: 41.2% vs. 62.5%, p = 0.022). The TT genotype increased the risk of PPCM by 4.6-fold. There was also a significant difference in the ACE DD genotype in the case group compared with that in the control group (26.5% vs. 9.1%, p = 0.021). DD genotypes increased the risk of PPCM by 3.6-fold. ACE levels were significantly higher in the DD genotype group than in the ID and II genotype groups (4,356.88 ± 232.44 pg/mL vs. 3,980.91 ± 77.79 pg/mL vs. 3,679.94 ± 325.77 pg/mL, p < 0.001).ConclusionThe TT genotype of GNB3 and the DD genotype of the ACE are likely to increase the risk of PPCM. Therefore, these polymorphisms may be predisposing risk factors for PPCM incidence. ACE levels were significantly higher in the DD genotype group, which certainly had clinical implications for the management of PPCM patients in the administration of ACE inhibitors as one of the therapy options. |
| first_indexed | 2024-04-09T19:29:37Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-09T19:29:37Z |
| publishDate | 2023-04-01 |
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| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-2392352efc5f49d693cac9d95e035a892023-04-05T04:42:38ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2023-04-011010.3389/fcvm.2023.10965141096514Association polymorphism of guanine nucleotide–binding protein β3 subunit (GNB3) C825T and insertion/deletion of the angiotensin-converting enzyme (ACE) gene with peripartum cardiomyopathyIvana Purnama Dewi0Ivana Purnama Dewi1Louisa Fadjri Kusuma Wardhani2Irma Maghfirah3Kristin Purnama Dewi4Kristin Purnama Dewi5Agus Subagjo6Mochamad Yusuf Alsagaff7Johanes Nugroho8Department of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University—Dr. Soetomo General Hospital, Surabaya, IndonesiaFaculty of Medicine, Duta Wacana Christian University, Yogyakarta, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University—Dr. Soetomo General Hospital, Surabaya, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University—Dr. Soetomo General Hospital, Surabaya, IndonesiaFaculty of Medicine, Duta Wacana Christian University, Yogyakarta, IndonesiaDepartment of Pulmonology and Respiratory Medicine, Faculty of Medicine, Airlangga University—Dr. Soetomo General Hospital, Surabaya, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University—Dr. Soetomo General Hospital, Surabaya, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University—Dr. Soetomo General Hospital, Surabaya, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University—Dr. Soetomo General Hospital, Surabaya, IndonesiaIntroductionPeripartum cardiomyopathy (PPCM) is a potentially life-threatening pregnancy-related heart disease. Genetic roles such as gene polymorphisms may relate to the etiology of PPCM. This study analyzes the association between single nucleotide gene polymorphism (SNP) guanine nucleotide–binding protein beta-3 subunit (GNB3) C825T and insertion/deletion (I/D) of the angiotensin-converting enzyme (ACE) gene with the incidence of PPCM.MethodsAn analytic observational study with a case–control design was conducted at the Integrated Cardiac Service Center of Dr. Soetomo General Hospital, Surabaya, Indonesia. PPCM patients of the case and control groups were enrolled. Baseline characteristic data were collected and blood samples were analyzed for SNP in the GNB3 C825T gene and for I/D in the ACE gene by using the polymerase chain reaction, restriction fragment length polymorphism, and Sanger sequencing. We also assessed ACE levels among different ACE genotypes using a sandwich-ELISA test.ResultsA total of 100 patients were included in this study, with 34 PPCM cases and 66 controls. There were significant differences in GNB3 TT and TC genotypes in the case group compared with that in the control group (TT: 35.3% vs. 10.6%, p = 0.003; TC: 41.2% vs. 62.5%, p = 0.022). The TT genotype increased the risk of PPCM by 4.6-fold. There was also a significant difference in the ACE DD genotype in the case group compared with that in the control group (26.5% vs. 9.1%, p = 0.021). DD genotypes increased the risk of PPCM by 3.6-fold. ACE levels were significantly higher in the DD genotype group than in the ID and II genotype groups (4,356.88 ± 232.44 pg/mL vs. 3,980.91 ± 77.79 pg/mL vs. 3,679.94 ± 325.77 pg/mL, p < 0.001).ConclusionThe TT genotype of GNB3 and the DD genotype of the ACE are likely to increase the risk of PPCM. Therefore, these polymorphisms may be predisposing risk factors for PPCM incidence. ACE levels were significantly higher in the DD genotype group, which certainly had clinical implications for the management of PPCM patients in the administration of ACE inhibitors as one of the therapy options.https://www.frontiersin.org/articles/10.3389/fcvm.2023.1096514/fullsingle nucleotide polymorphismperipartum cardiomyopathyPPCMguanine nucleotide–binding protein subunit β3angiotensin-converting enzyme |
| spellingShingle | Ivana Purnama Dewi Ivana Purnama Dewi Louisa Fadjri Kusuma Wardhani Irma Maghfirah Kristin Purnama Dewi Kristin Purnama Dewi Agus Subagjo Mochamad Yusuf Alsagaff Johanes Nugroho Association polymorphism of guanine nucleotide–binding protein β3 subunit (GNB3) C825T and insertion/deletion of the angiotensin-converting enzyme (ACE) gene with peripartum cardiomyopathy Frontiers in Cardiovascular Medicine single nucleotide polymorphism peripartum cardiomyopathy PPCM guanine nucleotide–binding protein subunit β3 angiotensin-converting enzyme |
| title | Association polymorphism of guanine nucleotide–binding protein β3 subunit (GNB3) C825T and insertion/deletion of the angiotensin-converting enzyme (ACE) gene with peripartum cardiomyopathy |
| title_full | Association polymorphism of guanine nucleotide–binding protein β3 subunit (GNB3) C825T and insertion/deletion of the angiotensin-converting enzyme (ACE) gene with peripartum cardiomyopathy |
| title_fullStr | Association polymorphism of guanine nucleotide–binding protein β3 subunit (GNB3) C825T and insertion/deletion of the angiotensin-converting enzyme (ACE) gene with peripartum cardiomyopathy |
| title_full_unstemmed | Association polymorphism of guanine nucleotide–binding protein β3 subunit (GNB3) C825T and insertion/deletion of the angiotensin-converting enzyme (ACE) gene with peripartum cardiomyopathy |
| title_short | Association polymorphism of guanine nucleotide–binding protein β3 subunit (GNB3) C825T and insertion/deletion of the angiotensin-converting enzyme (ACE) gene with peripartum cardiomyopathy |
| title_sort | association polymorphism of guanine nucleotide binding protein β3 subunit gnb3 c825t and insertion deletion of the angiotensin converting enzyme ace gene with peripartum cardiomyopathy |
| topic | single nucleotide polymorphism peripartum cardiomyopathy PPCM guanine nucleotide–binding protein subunit β3 angiotensin-converting enzyme |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1096514/full |
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