Tracking Mangrove Oil Bioremediation Approaches and Bacterial Diversity at Different Depths in an in situ Mesocosms System
In this study, oil spills were simulated in field-based mangrove mesocosms to compare the efficiency of bioremediation strategies and to characterize the presence of the alkB, ndo, assA, and bssA genes and the ecological structures of microbial communities in mangrove sediments at two different dept...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02107/full |
| _version_ | 1811264130316763136 |
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| author | Laís Feitosa Machado Deborah Catharine de Assis Leite Caio Tavora Coelho da Costa Rachid Jorge Eduardo Paes Edir Ferreira Martins Raquel Silva Peixoto Raquel Silva Peixoto Alexandre Soares Rosado Alexandre Soares Rosado |
| author_facet | Laís Feitosa Machado Deborah Catharine de Assis Leite Caio Tavora Coelho da Costa Rachid Jorge Eduardo Paes Edir Ferreira Martins Raquel Silva Peixoto Raquel Silva Peixoto Alexandre Soares Rosado Alexandre Soares Rosado |
| author_sort | Laís Feitosa Machado |
| collection | DOAJ |
| description | In this study, oil spills were simulated in field-based mangrove mesocosms to compare the efficiency of bioremediation strategies and to characterize the presence of the alkB, ndo, assA, and bssA genes and the ecological structures of microbial communities in mangrove sediments at two different depths, (D1) 1–10 cm and (D2) 25–35 cm. The results indicated that the hydrocarbon degradation efficiency was higher in superficial sediment layers, although no differences in the hydrocarbon degradation rates or in the abundances of the alkB and ndo genes were detected among the tested bioremediation strategies at this depth. Samples from the deeper layer exhibited higher abundances of the analyzed genes, except for assA and bssA, which were not detected in our samples. For all of the treatments and depths, the most abundant phyla were Proteobacteria, Firmicutes and Bacteroidetes, with Gammaproteobacteria, Flavobacteriales and Clostridiales being the most common classes. The indicator species analysis (ISA) results showed strong distinctions among microbial taxa in response to different treatments and in the two collection depths. Our results indicated a high efficiency of the monitored natural attenuation (MNA) for oil consumption in the tested mangrove sediments, revealing the potential of this strategy for environmental decontamination and suggesting that environmental and ecological factors may select for specific bacterial populations in distinct niches. |
| first_indexed | 2024-04-12T19:57:26Z |
| format | Article |
| id | doaj.art-23937fbb349549d89e927eb6d5d3f2b2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T19:57:26Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-23937fbb349549d89e927eb6d5d3f2b22022-12-22T03:18:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-09-011010.3389/fmicb.2019.02107462758Tracking Mangrove Oil Bioremediation Approaches and Bacterial Diversity at Different Depths in an in situ Mesocosms SystemLaís Feitosa Machado0Deborah Catharine de Assis Leite1Caio Tavora Coelho da Costa Rachid2Jorge Eduardo Paes3Edir Ferreira Martins4Raquel Silva Peixoto5Raquel Silva Peixoto6Alexandre Soares Rosado7Alexandre Soares Rosado8Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilInstitute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilInstitute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilResearch Center Leopoldo Américo Miguez de Mello, Rio de Janeiro, BrazilInstitute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilInstitute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilIMAM-AquaRio – Rio de Janeiro Aquarium Research Center, Rio de Janeiro, BrazilInstitute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilIMAM-AquaRio – Rio de Janeiro Aquarium Research Center, Rio de Janeiro, BrazilIn this study, oil spills were simulated in field-based mangrove mesocosms to compare the efficiency of bioremediation strategies and to characterize the presence of the alkB, ndo, assA, and bssA genes and the ecological structures of microbial communities in mangrove sediments at two different depths, (D1) 1–10 cm and (D2) 25–35 cm. The results indicated that the hydrocarbon degradation efficiency was higher in superficial sediment layers, although no differences in the hydrocarbon degradation rates or in the abundances of the alkB and ndo genes were detected among the tested bioremediation strategies at this depth. Samples from the deeper layer exhibited higher abundances of the analyzed genes, except for assA and bssA, which were not detected in our samples. For all of the treatments and depths, the most abundant phyla were Proteobacteria, Firmicutes and Bacteroidetes, with Gammaproteobacteria, Flavobacteriales and Clostridiales being the most common classes. The indicator species analysis (ISA) results showed strong distinctions among microbial taxa in response to different treatments and in the two collection depths. Our results indicated a high efficiency of the monitored natural attenuation (MNA) for oil consumption in the tested mangrove sediments, revealing the potential of this strategy for environmental decontamination and suggesting that environmental and ecological factors may select for specific bacterial populations in distinct niches.https://www.frontiersin.org/article/10.3389/fmicb.2019.02107/fullbioremediationmangrovemesocosmoil-degrading genesbacterial diversity |
| spellingShingle | Laís Feitosa Machado Deborah Catharine de Assis Leite Caio Tavora Coelho da Costa Rachid Jorge Eduardo Paes Edir Ferreira Martins Raquel Silva Peixoto Raquel Silva Peixoto Alexandre Soares Rosado Alexandre Soares Rosado Tracking Mangrove Oil Bioremediation Approaches and Bacterial Diversity at Different Depths in an in situ Mesocosms System Frontiers in Microbiology bioremediation mangrove mesocosm oil-degrading genes bacterial diversity |
| title | Tracking Mangrove Oil Bioremediation Approaches and Bacterial Diversity at Different Depths in an in situ Mesocosms System |
| title_full | Tracking Mangrove Oil Bioremediation Approaches and Bacterial Diversity at Different Depths in an in situ Mesocosms System |
| title_fullStr | Tracking Mangrove Oil Bioremediation Approaches and Bacterial Diversity at Different Depths in an in situ Mesocosms System |
| title_full_unstemmed | Tracking Mangrove Oil Bioremediation Approaches and Bacterial Diversity at Different Depths in an in situ Mesocosms System |
| title_short | Tracking Mangrove Oil Bioremediation Approaches and Bacterial Diversity at Different Depths in an in situ Mesocosms System |
| title_sort | tracking mangrove oil bioremediation approaches and bacterial diversity at different depths in an in situ mesocosms system |
| topic | bioremediation mangrove mesocosm oil-degrading genes bacterial diversity |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02107/full |
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