Construction of <i>P</i><i>olygonatum sibiricum</i> Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity
Although selenium nanoparticles (SeNPs) have attracted great attention due to their potential antioxidant activity and low toxicity, the application of SeNPs is still restricted by their poor stability. A combination of polysaccharides and SeNPs is an effective strategy to overcome the limitations....
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/2/240 |
| _version_ | 1797483247501836288 |
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| author | Wanwen Chen Hao Cheng Wenshui Xia |
| author_facet | Wanwen Chen Hao Cheng Wenshui Xia |
| author_sort | Wanwen Chen |
| collection | DOAJ |
| description | Although selenium nanoparticles (SeNPs) have attracted great attention due to their potential antioxidant activity and low toxicity, the application of SeNPs is still restricted by their poor stability. A combination of polysaccharides and SeNPs is an effective strategy to overcome the limitations. In this study, <i>Polygonatum sibiricum</i> polysaccharide (PSP) was used as a stabilizer to fabricate SeNPs under a simple redox system. Dynamic light scattering, transmission electron microscopy, energy dispersive X-ray, ultraviolet-visible spectroscopy, Fourier transform infrared, and X-ray photoelectron spectrometer were applied to characterize the synthesized PSP-SeNPs. The stability and the antioxidant activity of PSP-SeNPs were also investigated. The results revealed that the zero-valent and well-dispersed spherical PSP-SeNPs with an average size of 105 nm and a negative ζ-potential of −34.9 mV were successfully synthesized using 0.1 mg/mL PSP as a stabilizer. The prepared PSP-SeNPs were stable for 30 days at 4 °C. The decoration of the nanoparticle surface with PSP significantly improved the free radical scavenging ability of SeNPs. Compared to the H<sub>2</sub>O<sub>2</sub>-induced oxidative stress model group, the viability of PC-12 cells pretreated with 20 μg/mL PSP-SeNPs increased from 56% to 98%. Moreover, PSP-SeNPs exhibited a higher protective effect on the H<sub>2</sub>O<sub>2</sub>-induced oxidative damage on PC-12 cells and lower cytotoxicity than sodium selenite and SeNPs. In summary, these results suggest the great potential of PSP-SeNPs as a novel antioxidant agent in the food or nutraceuticals area. |
| first_indexed | 2024-03-09T22:45:13Z |
| format | Article |
| id | doaj.art-239543d1b539438e870f6d06e7d5fe22 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T22:45:13Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-239543d1b539438e870f6d06e7d5fe222023-11-23T18:30:38ZengMDPI AGAntioxidants2076-39212022-01-0111224010.3390/antiox11020240Construction of <i>P</i><i>olygonatum sibiricum</i> Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant ActivityWanwen Chen0Hao Cheng1Wenshui Xia2State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaAlthough selenium nanoparticles (SeNPs) have attracted great attention due to their potential antioxidant activity and low toxicity, the application of SeNPs is still restricted by their poor stability. A combination of polysaccharides and SeNPs is an effective strategy to overcome the limitations. In this study, <i>Polygonatum sibiricum</i> polysaccharide (PSP) was used as a stabilizer to fabricate SeNPs under a simple redox system. Dynamic light scattering, transmission electron microscopy, energy dispersive X-ray, ultraviolet-visible spectroscopy, Fourier transform infrared, and X-ray photoelectron spectrometer were applied to characterize the synthesized PSP-SeNPs. The stability and the antioxidant activity of PSP-SeNPs were also investigated. The results revealed that the zero-valent and well-dispersed spherical PSP-SeNPs with an average size of 105 nm and a negative ζ-potential of −34.9 mV were successfully synthesized using 0.1 mg/mL PSP as a stabilizer. The prepared PSP-SeNPs were stable for 30 days at 4 °C. The decoration of the nanoparticle surface with PSP significantly improved the free radical scavenging ability of SeNPs. Compared to the H<sub>2</sub>O<sub>2</sub>-induced oxidative stress model group, the viability of PC-12 cells pretreated with 20 μg/mL PSP-SeNPs increased from 56% to 98%. Moreover, PSP-SeNPs exhibited a higher protective effect on the H<sub>2</sub>O<sub>2</sub>-induced oxidative damage on PC-12 cells and lower cytotoxicity than sodium selenite and SeNPs. In summary, these results suggest the great potential of PSP-SeNPs as a novel antioxidant agent in the food or nutraceuticals area.https://www.mdpi.com/2076-3921/11/2/240selenium nanoparticles<i>Polygonatum sibiricum</i> polysaccharidestabilityantioxidant |
| spellingShingle | Wanwen Chen Hao Cheng Wenshui Xia Construction of <i>P</i><i>olygonatum sibiricum</i> Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity Antioxidants selenium nanoparticles <i>Polygonatum sibiricum</i> polysaccharide stability antioxidant |
| title | Construction of <i>P</i><i>olygonatum sibiricum</i> Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity |
| title_full | Construction of <i>P</i><i>olygonatum sibiricum</i> Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity |
| title_fullStr | Construction of <i>P</i><i>olygonatum sibiricum</i> Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity |
| title_full_unstemmed | Construction of <i>P</i><i>olygonatum sibiricum</i> Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity |
| title_short | Construction of <i>P</i><i>olygonatum sibiricum</i> Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity |
| title_sort | construction of i p i i olygonatum sibiricum i polysaccharide functionalized selenium nanoparticles for the enhancement of stability and antioxidant activity |
| topic | selenium nanoparticles <i>Polygonatum sibiricum</i> polysaccharide stability antioxidant |
| url | https://www.mdpi.com/2076-3921/11/2/240 |
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