Regiospecific Hydrogenation of Bromochalcone by Unconventional Yeast Strains
This research aimed to select yeast strains capable of the biotransformation of selected 2′-hydroxybromochalcones. Small-scale biotransformations were carried out using four substrates obtained by chemical synthesis (2′-hydroxy-2″-bromochalcone, 2′-hydroxy-3″-bromochalcone, 2′-hydroxy-4″-bromochalco...
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2022-06-01
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author | Mateusz Łużny Dagmara Kaczanowska Barbara Gawdzik Alicja Wzorek Aleksandra Pawlak Bożena Obmińska-Mrukowicz Monika Dymarska Ewa Kozłowska Edyta Kostrzewa-Susłow Tomasz Janeczko |
author_facet | Mateusz Łużny Dagmara Kaczanowska Barbara Gawdzik Alicja Wzorek Aleksandra Pawlak Bożena Obmińska-Mrukowicz Monika Dymarska Ewa Kozłowska Edyta Kostrzewa-Susłow Tomasz Janeczko |
author_sort | Mateusz Łużny |
collection | DOAJ |
description | This research aimed to select yeast strains capable of the biotransformation of selected 2′-hydroxybromochalcones. Small-scale biotransformations were carried out using four substrates obtained by chemical synthesis (2′-hydroxy-2″-bromochalcone, 2′-hydroxy-3″-bromochalcone, 2′-hydroxy-4″-bromochalcone and 2′-hydroxy-5′-bromochalcone) and eight strains of non-conventional yeasts. Screening allowed for the determination of the substrate specificity of selected microorganisms and the selection of biocatalysts that carried out the hydrogenation of tested compounds in the most effective way. It was found that the position of the bromine atom has a crucial influence on the degree of substrate conversion by the tested yeast strains. As a result of the biotransformation of the 2′-hydroxybromochalcones, the corresponding 2′-hydroxybromodihydrochalcones were obtained. The products obtained belong to the group of compounds with high potential as precursors of sweet substances. |
first_indexed | 2024-03-09T22:56:50Z |
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id | doaj.art-239573dce57f46c685ee2bb8e3d637e7 |
institution | Directory Open Access Journal |
issn | 1420-3049 |
language | English |
last_indexed | 2024-03-09T22:56:50Z |
publishDate | 2022-06-01 |
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series | Molecules |
spelling | doaj.art-239573dce57f46c685ee2bb8e3d637e72023-11-23T18:09:26ZengMDPI AGMolecules1420-30492022-06-012712368110.3390/molecules27123681Regiospecific Hydrogenation of Bromochalcone by Unconventional Yeast StrainsMateusz Łużny0Dagmara Kaczanowska1Barbara Gawdzik2Alicja Wzorek3Aleksandra Pawlak4Bożena Obmińska-Mrukowicz5Monika Dymarska6Ewa Kozłowska7Edyta Kostrzewa-Susłow8Tomasz Janeczko9Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, PolandDepartment of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, PolandInstitute of Chemistry, Jan Kochanowski University in Kielce, Uniwersytecka 7, 25-406 Kielce, PolandInstitute of Chemistry, Jan Kochanowski University in Kielce, Uniwersytecka 7, 25-406 Kielce, PolandDepartment of Pharmacology and Toxicology, Wrocław University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wrocław, PolandDepartment of Pharmacology and Toxicology, Wrocław University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wrocław, PolandDepartment of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, PolandDepartment of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, PolandDepartment of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, PolandDepartment of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, PolandThis research aimed to select yeast strains capable of the biotransformation of selected 2′-hydroxybromochalcones. Small-scale biotransformations were carried out using four substrates obtained by chemical synthesis (2′-hydroxy-2″-bromochalcone, 2′-hydroxy-3″-bromochalcone, 2′-hydroxy-4″-bromochalcone and 2′-hydroxy-5′-bromochalcone) and eight strains of non-conventional yeasts. Screening allowed for the determination of the substrate specificity of selected microorganisms and the selection of biocatalysts that carried out the hydrogenation of tested compounds in the most effective way. It was found that the position of the bromine atom has a crucial influence on the degree of substrate conversion by the tested yeast strains. As a result of the biotransformation of the 2′-hydroxybromochalcones, the corresponding 2′-hydroxybromodihydrochalcones were obtained. The products obtained belong to the group of compounds with high potential as precursors of sweet substances.https://www.mdpi.com/1420-3049/27/12/3681biotransformationssweetenersdihydrochalconesyeast |
spellingShingle | Mateusz Łużny Dagmara Kaczanowska Barbara Gawdzik Alicja Wzorek Aleksandra Pawlak Bożena Obmińska-Mrukowicz Monika Dymarska Ewa Kozłowska Edyta Kostrzewa-Susłow Tomasz Janeczko Regiospecific Hydrogenation of Bromochalcone by Unconventional Yeast Strains Molecules biotransformations sweeteners dihydrochalcones yeast |
title | Regiospecific Hydrogenation of Bromochalcone by Unconventional Yeast Strains |
title_full | Regiospecific Hydrogenation of Bromochalcone by Unconventional Yeast Strains |
title_fullStr | Regiospecific Hydrogenation of Bromochalcone by Unconventional Yeast Strains |
title_full_unstemmed | Regiospecific Hydrogenation of Bromochalcone by Unconventional Yeast Strains |
title_short | Regiospecific Hydrogenation of Bromochalcone by Unconventional Yeast Strains |
title_sort | regiospecific hydrogenation of bromochalcone by unconventional yeast strains |
topic | biotransformations sweeteners dihydrochalcones yeast |
url | https://www.mdpi.com/1420-3049/27/12/3681 |
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