SCA1+ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions
Stem cell antigen 1-positive (SCA1+) cells (SPCs) have been investigated in cell-based cardiac repair and pharmacological research, although improved cardiac function after injection has been variable and the mode of action remains unclear. Circadian (24-hr) rhythms are biorhythms regulated by molec...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-09-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221367111730320X |
| _version_ | 1818012664922636288 |
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| author | Bastiaan C. Du Pré Evelyne J. Demkes Dries A.M. Feyen Pieterjan Dierickx Sandra Crnko Bart J.M. Kok Joost P.G. Sluijter Pieter A. Doevendans Marc A. Vos Toon A.B. Van Veen Linda W. Van Laake |
| author_facet | Bastiaan C. Du Pré Evelyne J. Demkes Dries A.M. Feyen Pieterjan Dierickx Sandra Crnko Bart J.M. Kok Joost P.G. Sluijter Pieter A. Doevendans Marc A. Vos Toon A.B. Van Veen Linda W. Van Laake |
| author_sort | Bastiaan C. Du Pré |
| collection | DOAJ |
| description | Stem cell antigen 1-positive (SCA1+) cells (SPCs) have been investigated in cell-based cardiac repair and pharmacological research, although improved cardiac function after injection has been variable and the mode of action remains unclear. Circadian (24-hr) rhythms are biorhythms regulated by molecular clocks that play an important role in (patho)physiology. Here, we describe (1) the presence of a molecular circadian clock in SPCs and (2) circadian rhythmicity in SPC function. We isolated SPCs from human fetal heart and found that these cells possess a molecular clock based on typical oscillations in core clock components BMAL1 and CRY1. Functional analyses revealed that circadian rhythmicity also governs SPC proliferation, stress tolerance, and growth factor release, with large differences between peaks and troughs. We conclude that SPCs contain a circadian molecular clock that controls crucial cellular functions. Taking circadian rhythms into account may improve reproducibility and outcome of research and therapies using SPCs. |
| first_indexed | 2024-04-14T06:23:08Z |
| format | Article |
| id | doaj.art-23974ebef2f04d14a6c929473df2d1c7 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-14T06:23:08Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-23974ebef2f04d14a6c929473df2d1c72022-12-22T02:07:56ZengElsevierStem Cell Reports2213-67112017-09-019376276910.1016/j.stemcr.2017.07.010SCA1+ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular FunctionsBastiaan C. Du Pré0Evelyne J. Demkes1Dries A.M. Feyen2Pieterjan Dierickx3Sandra Crnko4Bart J.M. Kok5Joost P.G. Sluijter6Pieter A. Doevendans7Marc A. Vos8Toon A.B. Van Veen9Linda W. Van Laake10Department of Medical Physiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Medical Physiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Cardiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Cardiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Cardiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Medical Physiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Cardiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Cardiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Medical Physiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Medical Physiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsDepartment of Cardiology, Division of Heart and Lungs, and Regenerative Medicine Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the NetherlandsStem cell antigen 1-positive (SCA1+) cells (SPCs) have been investigated in cell-based cardiac repair and pharmacological research, although improved cardiac function after injection has been variable and the mode of action remains unclear. Circadian (24-hr) rhythms are biorhythms regulated by molecular clocks that play an important role in (patho)physiology. Here, we describe (1) the presence of a molecular circadian clock in SPCs and (2) circadian rhythmicity in SPC function. We isolated SPCs from human fetal heart and found that these cells possess a molecular clock based on typical oscillations in core clock components BMAL1 and CRY1. Functional analyses revealed that circadian rhythmicity also governs SPC proliferation, stress tolerance, and growth factor release, with large differences between peaks and troughs. We conclude that SPCs contain a circadian molecular clock that controls crucial cellular functions. Taking circadian rhythms into account may improve reproducibility and outcome of research and therapies using SPCs.http://www.sciencedirect.com/science/article/pii/S221367111730320Xcircadian rhythmclockstem cellprogenitor cellSCA1heart |
| spellingShingle | Bastiaan C. Du Pré Evelyne J. Demkes Dries A.M. Feyen Pieterjan Dierickx Sandra Crnko Bart J.M. Kok Joost P.G. Sluijter Pieter A. Doevendans Marc A. Vos Toon A.B. Van Veen Linda W. Van Laake SCA1+ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions Stem Cell Reports circadian rhythm clock stem cell progenitor cell SCA1 heart |
| title | SCA1+ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions |
| title_full | SCA1+ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions |
| title_fullStr | SCA1+ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions |
| title_full_unstemmed | SCA1+ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions |
| title_short | SCA1+ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions |
| title_sort | sca1 cells from the heart possess a molecular circadian clock and display circadian oscillations in cellular functions |
| topic | circadian rhythm clock stem cell progenitor cell SCA1 heart |
| url | http://www.sciencedirect.com/science/article/pii/S221367111730320X |
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