Amlexanox Exhibits Cardioprotective Effects in 5/6 Nephrectomized Rats
Abstract Cardiorenal syndrome is a life-threatening condition. The aim of the current study was to determine the cardioprotective effects of amlexanox in 5/6 nephrectomized rats. Rats were randomly assigned to three groups: sham, 5/6 nephrectomized rats, and amlexanox-treated 5/6 nephrectomized grou...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2023-01-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100845&tlng=en |
| _version_ | 1797957070671052800 |
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| author | Rasha Mohamed Sabry Mohamed Mohamed Shimaa Mostafa Elshazly Nevertyty Mohamed Mahmoud |
| author_facet | Rasha Mohamed Sabry Mohamed Mohamed Shimaa Mostafa Elshazly Nevertyty Mohamed Mahmoud |
| author_sort | Rasha Mohamed Sabry Mohamed Mohamed |
| collection | DOAJ |
| description | Abstract Cardiorenal syndrome is a life-threatening condition. The aim of the current study was to determine the cardioprotective effects of amlexanox in 5/6 nephrectomized rats. Rats were randomly assigned to three groups: sham, 5/6 nephrectomized rats, and amlexanox-treated 5/6 nephrectomized group. Amlexanox (25 mg/kg/day, i.p.) administration was started just after surgery and continued for 10 weeks. After treatment, kidney function (serum creatinine and urea) and blood pressure (systolic and diastolic) were measured. Heart weight (normalized to tibial length) and fibrosis area percentage were measured. Serum brain natriuretic peptide (BNP, heart failure marker) and cardiac levels of β1-adrenergic receptor (β1AR), β-arrestin-2, phosphatidylinositol-4,5-bisphosphate (PIP2), diacylglycerol (DAG), pS473 Akt (a survival marker), and caspase-3 activity (an apoptosis marker) were also measured. The 5/6 nephrectomy caused renal impairment, cardiac fibrosis, apoptosis, and heart failure indicated by down- regulation of cardiac β1AR down-stream signals compared with those in the sham group. Interestingly, amlexanox significantly reduced all cardiopathological changes induced after 10 weeks of 5/6 nephrectomy. Amlexanox showed potent cardiac antifibrotic and antiapoptotic effects in 5/6 nephrectomized rats, which were associated with reduced heart failure. To our knowledge, this is the first study that addresses the potent in vivo cardioprotective effects of amlexanox. |
| first_indexed | 2024-04-10T23:58:38Z |
| format | Article |
| id | doaj.art-2398d402c8264e559c6b6338a269f0f4 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-10T23:58:38Z |
| publishDate | 2023-01-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-2398d402c8264e559c6b6338a269f0f42023-01-10T07:34:31ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902023-01-015810.1590/s2175-97902022e20978Amlexanox Exhibits Cardioprotective Effects in 5/6 Nephrectomized RatsRasha Mohamed Sabry Mohamed Mohamedhttps://orcid.org/0000-0002-4173-4399Shimaa Mostafa ElshazlyNevertyty Mohamed MahmoudAbstract Cardiorenal syndrome is a life-threatening condition. The aim of the current study was to determine the cardioprotective effects of amlexanox in 5/6 nephrectomized rats. Rats were randomly assigned to three groups: sham, 5/6 nephrectomized rats, and amlexanox-treated 5/6 nephrectomized group. Amlexanox (25 mg/kg/day, i.p.) administration was started just after surgery and continued for 10 weeks. After treatment, kidney function (serum creatinine and urea) and blood pressure (systolic and diastolic) were measured. Heart weight (normalized to tibial length) and fibrosis area percentage were measured. Serum brain natriuretic peptide (BNP, heart failure marker) and cardiac levels of β1-adrenergic receptor (β1AR), β-arrestin-2, phosphatidylinositol-4,5-bisphosphate (PIP2), diacylglycerol (DAG), pS473 Akt (a survival marker), and caspase-3 activity (an apoptosis marker) were also measured. The 5/6 nephrectomy caused renal impairment, cardiac fibrosis, apoptosis, and heart failure indicated by down- regulation of cardiac β1AR down-stream signals compared with those in the sham group. Interestingly, amlexanox significantly reduced all cardiopathological changes induced after 10 weeks of 5/6 nephrectomy. Amlexanox showed potent cardiac antifibrotic and antiapoptotic effects in 5/6 nephrectomized rats, which were associated with reduced heart failure. To our knowledge, this is the first study that addresses the potent in vivo cardioprotective effects of amlexanox.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100845&tlng=enAmlexanoxCardiorenal syndromeβ-Arrestin-2 |
| spellingShingle | Rasha Mohamed Sabry Mohamed Mohamed Shimaa Mostafa Elshazly Nevertyty Mohamed Mahmoud Amlexanox Exhibits Cardioprotective Effects in 5/6 Nephrectomized Rats Brazilian Journal of Pharmaceutical Sciences Amlexanox Cardiorenal syndrome β-Arrestin-2 |
| title | Amlexanox Exhibits Cardioprotective Effects in 5/6 Nephrectomized Rats |
| title_full | Amlexanox Exhibits Cardioprotective Effects in 5/6 Nephrectomized Rats |
| title_fullStr | Amlexanox Exhibits Cardioprotective Effects in 5/6 Nephrectomized Rats |
| title_full_unstemmed | Amlexanox Exhibits Cardioprotective Effects in 5/6 Nephrectomized Rats |
| title_short | Amlexanox Exhibits Cardioprotective Effects in 5/6 Nephrectomized Rats |
| title_sort | amlexanox exhibits cardioprotective effects in 5 6 nephrectomized rats |
| topic | Amlexanox Cardiorenal syndrome β-Arrestin-2 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100845&tlng=en |
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