Effect of Ciprofloxacin-Loaded Niosomes on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> Biofilm Formation
Infections caused by bacterial biofilms represent a global health problem, causing considerable patient morbidity and mortality in addition to an economic burden. <i>Escherichia coli</i>, <i>Staphylococcus aureus,</i> and other medically relevant bacterial strains colonize cl...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/14/12/2662 |
| _version_ | 1797455702141173760 |
|---|---|
| author | Linda Maurizi Jacopo Forte Maria Grazia Ammendolia Patrizia Nadia Hanieh Antonietta Lucia Conte Michela Relucenti Orlando Donfrancesco Caterina Ricci Federica Rinaldi Carlotta Marianecci Maria Carafa Catia Longhi |
| author_facet | Linda Maurizi Jacopo Forte Maria Grazia Ammendolia Patrizia Nadia Hanieh Antonietta Lucia Conte Michela Relucenti Orlando Donfrancesco Caterina Ricci Federica Rinaldi Carlotta Marianecci Maria Carafa Catia Longhi |
| author_sort | Linda Maurizi |
| collection | DOAJ |
| description | Infections caused by bacterial biofilms represent a global health problem, causing considerable patient morbidity and mortality in addition to an economic burden. <i>Escherichia coli</i>, <i>Staphylococcus aureus,</i> and other medically relevant bacterial strains colonize clinical surfaces and medical devices via biofilm in which bacterial cells are protected from the action of the immune system, disinfectants, and antibiotics. Several approaches have been investigated to inhibit and disperse bacterial biofilms, and the use of drug delivery could represent a fascinating strategy. Ciprofloxacin (CIP), which belongs to the class of fluoroquinolones, has been extensively used against various bacterial infections, and its loading in nanocarriers, such as niosomes, could support the CIP antibiofilm activity. Niosomes, composed of two surfactants (Tween 85 and Span 80) without the presence of cholesterol, are prepared and characterized considering the following features: hydrodynamic diameter, ζ-potential, morphology, vesicle bilayer characteristics, physical-chemical stability, and biological efficacy. The obtained results suggest that: (i) niosomes by surfactants in the absence of cholesterol are formed, can entrap CIP, and are stable over time and in artificial biological media; (ii) the CIP inclusion in nanocarriers increase its stability, with respect to free drug; (iii) niosomes preparations were able to induce a relevant inhibition of biofilm formation. |
| first_indexed | 2024-03-09T15:58:07Z |
| format | Article |
| id | doaj.art-239a3d44cfca4a97a59ac290cbfa25c4 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T15:58:07Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-239a3d44cfca4a97a59ac290cbfa25c42023-11-24T17:19:49ZengMDPI AGPharmaceutics1999-49232022-11-011412266210.3390/pharmaceutics14122662Effect of Ciprofloxacin-Loaded Niosomes on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> Biofilm FormationLinda Maurizi0Jacopo Forte1Maria Grazia Ammendolia2Patrizia Nadia Hanieh3Antonietta Lucia Conte4Michela Relucenti5Orlando Donfrancesco6Caterina Ricci7Federica Rinaldi8Carlotta Marianecci9Maria Carafa10Catia Longhi11Dipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di Roma, Piazzale Aldo Moro, 5-00185 Roma, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro, 5-00185 Roma, ItalyCentro Nazionale Tecnologie Innovative in Sanità Pubblica, Istituto Superiore di Sanità, Viale Regina Elena, 299-00161 Roma, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro, 5-00185 Roma, ItalyDipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di Roma, Piazzale Aldo Moro, 5-00185 Roma, ItalyDipartimento di Scienze Anatomiche, Istologiche, Medico-Legali e dell’Apparato locomotore, Sapienza Università di Roma, Via Alfonso Borelli, 50-00161 Roma, ItalyDipartimento di Scienze Anatomiche, Istologiche, Medico-Legali e dell’Apparato locomotore, Sapienza Università di Roma, Via Alfonso Borelli, 50-00161 Roma, ItalyDipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università di Milano, Via Fratelli Cervi, 93-20090 Milano, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro, 5-00185 Roma, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro, 5-00185 Roma, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro, 5-00185 Roma, ItalyDipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di Roma, Piazzale Aldo Moro, 5-00185 Roma, ItalyInfections caused by bacterial biofilms represent a global health problem, causing considerable patient morbidity and mortality in addition to an economic burden. <i>Escherichia coli</i>, <i>Staphylococcus aureus,</i> and other medically relevant bacterial strains colonize clinical surfaces and medical devices via biofilm in which bacterial cells are protected from the action of the immune system, disinfectants, and antibiotics. Several approaches have been investigated to inhibit and disperse bacterial biofilms, and the use of drug delivery could represent a fascinating strategy. Ciprofloxacin (CIP), which belongs to the class of fluoroquinolones, has been extensively used against various bacterial infections, and its loading in nanocarriers, such as niosomes, could support the CIP antibiofilm activity. Niosomes, composed of two surfactants (Tween 85 and Span 80) without the presence of cholesterol, are prepared and characterized considering the following features: hydrodynamic diameter, ζ-potential, morphology, vesicle bilayer characteristics, physical-chemical stability, and biological efficacy. The obtained results suggest that: (i) niosomes by surfactants in the absence of cholesterol are formed, can entrap CIP, and are stable over time and in artificial biological media; (ii) the CIP inclusion in nanocarriers increase its stability, with respect to free drug; (iii) niosomes preparations were able to induce a relevant inhibition of biofilm formation.https://www.mdpi.com/1999-4923/14/12/2662niosomesdrug deliveryciprofloxacinanti biofilm activitybladder cells |
| spellingShingle | Linda Maurizi Jacopo Forte Maria Grazia Ammendolia Patrizia Nadia Hanieh Antonietta Lucia Conte Michela Relucenti Orlando Donfrancesco Caterina Ricci Federica Rinaldi Carlotta Marianecci Maria Carafa Catia Longhi Effect of Ciprofloxacin-Loaded Niosomes on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> Biofilm Formation Pharmaceutics niosomes drug delivery ciprofloxacin anti biofilm activity bladder cells |
| title | Effect of Ciprofloxacin-Loaded Niosomes on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> Biofilm Formation |
| title_full | Effect of Ciprofloxacin-Loaded Niosomes on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> Biofilm Formation |
| title_fullStr | Effect of Ciprofloxacin-Loaded Niosomes on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> Biofilm Formation |
| title_full_unstemmed | Effect of Ciprofloxacin-Loaded Niosomes on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> Biofilm Formation |
| title_short | Effect of Ciprofloxacin-Loaded Niosomes on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> Biofilm Formation |
| title_sort | effect of ciprofloxacin loaded niosomes on i escherichia coli i and i staphylococcus aureus i biofilm formation |
| topic | niosomes drug delivery ciprofloxacin anti biofilm activity bladder cells |
| url | https://www.mdpi.com/1999-4923/14/12/2662 |
| work_keys_str_mv | AT lindamaurizi effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT jacopoforte effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT mariagraziaammendolia effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT patrizianadiahanieh effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT antoniettaluciaconte effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT michelarelucenti effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT orlandodonfrancesco effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT caterinaricci effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT federicarinaldi effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT carlottamarianecci effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT mariacarafa effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation AT catialonghi effectofciprofloxacinloadedniosomesoniescherichiacoliiandistaphylococcusaureusibiofilmformation |