Diesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β<sub>1</sub>/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in Mice
Diesel exhaust particulates (DEP) adversely affect the respiratory system and exacerbate lung diseases, resulting in high mortality rates. However, its pathogenesis is complicated, and the mechanisms involved are incompletely understood. We investigated the effects of DEP pre-exposure on lipopolysac...
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/2218-273X/11/1/67 |
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| author | Dong Im Kim Mi-Kyung Song Kyuhong Lee |
| author_facet | Dong Im Kim Mi-Kyung Song Kyuhong Lee |
| author_sort | Dong Im Kim |
| collection | DOAJ |
| description | Diesel exhaust particulates (DEP) adversely affect the respiratory system and exacerbate lung diseases, resulting in high mortality rates. However, its pathogenesis is complicated, and the mechanisms involved are incompletely understood. We investigated the effects of DEP pre-exposure on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and identified the roles of interleukin (IL)-17 in mice. Mice were divided into vehicle control, DEP, LPS, and DEP pre-exposed and LPS-instilled groups. Pre-exposure to DEP enhanced the number of total cells, neutrophils, and lymphocytes in the BAL fluid of LPS-instilled mice. Pre-exposure to DEP synergistically exacerbated pulmonary acute lung inflammation and granulomatous inflammation/pulmonary fibrosis, concomitant with the enhanced expression of inflammatory cytokines in the BAL fluid and of collagen I and TGF-β<sub>1</sub> in the lungs of LPS-instilled mice. The number of TGF-β<sub>1</sub>-positive cells in the DEP pre-exposed and LPS-instilled group was higher than that in the LPS group. The expression of NLR family pyrin domain containing 3 (NLRP3) inflammasome components was markedly increased in the DEP pre-exposed and LPS-instilled group. IL-17 levels in the BAL fluid and IL-17-positive cells in the lungs were significantly increased by pre-exposure to DEP in the LPS-induced group compared to that in the DEP or LPS group. These results suggest that DEP predominantly contributes to fibrotic lung disease in LPS-related acute lung injury by upregulating IL-17 cytokine-mediated collagen I and TGF-β<sub>1</sub> and, at least in part, by activating LPS-induced NLRP3 inflammasome signaling. The study should be useful in devising better strategies for prevention and management of ALI. |
| first_indexed | 2024-03-09T05:57:01Z |
| format | Article |
| id | doaj.art-239adc46ad054dc0850433f504d0d20c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T05:57:01Z |
| publishDate | 2021-01-01 |
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| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-239adc46ad054dc0850433f504d0d20c2023-12-03T12:12:22ZengMDPI AGBiomolecules2218-273X2021-01-011116710.3390/biom11010067Diesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β<sub>1</sub>/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in MiceDong Im Kim0Mi-Kyung Song1Kyuhong Lee2National Center for Efficacy Evaluation of Respiratory Disease Products, Korea Institute of Toxicology, 30 Baehak1-gil, Jeongeup 56212, KoreaNational Center for Efficacy Evaluation of Respiratory Disease Products, Korea Institute of Toxicology, 30 Baehak1-gil, Jeongeup 56212, KoreaNational Center for Efficacy Evaluation of Respiratory Disease Products, Korea Institute of Toxicology, 30 Baehak1-gil, Jeongeup 56212, KoreaDiesel exhaust particulates (DEP) adversely affect the respiratory system and exacerbate lung diseases, resulting in high mortality rates. However, its pathogenesis is complicated, and the mechanisms involved are incompletely understood. We investigated the effects of DEP pre-exposure on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and identified the roles of interleukin (IL)-17 in mice. Mice were divided into vehicle control, DEP, LPS, and DEP pre-exposed and LPS-instilled groups. Pre-exposure to DEP enhanced the number of total cells, neutrophils, and lymphocytes in the BAL fluid of LPS-instilled mice. Pre-exposure to DEP synergistically exacerbated pulmonary acute lung inflammation and granulomatous inflammation/pulmonary fibrosis, concomitant with the enhanced expression of inflammatory cytokines in the BAL fluid and of collagen I and TGF-β<sub>1</sub> in the lungs of LPS-instilled mice. The number of TGF-β<sub>1</sub>-positive cells in the DEP pre-exposed and LPS-instilled group was higher than that in the LPS group. The expression of NLR family pyrin domain containing 3 (NLRP3) inflammasome components was markedly increased in the DEP pre-exposed and LPS-instilled group. IL-17 levels in the BAL fluid and IL-17-positive cells in the lungs were significantly increased by pre-exposure to DEP in the LPS-induced group compared to that in the DEP or LPS group. These results suggest that DEP predominantly contributes to fibrotic lung disease in LPS-related acute lung injury by upregulating IL-17 cytokine-mediated collagen I and TGF-β<sub>1</sub> and, at least in part, by activating LPS-induced NLRP3 inflammasome signaling. The study should be useful in devising better strategies for prevention and management of ALI.https://www.mdpi.com/2218-273X/11/1/67diesel exhaust particulateacute lung injurylung fibrosisNLRP3IL-17TGF-β<sub>1</sub> |
| spellingShingle | Dong Im Kim Mi-Kyung Song Kyuhong Lee Diesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β<sub>1</sub>/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in Mice Biomolecules diesel exhaust particulate acute lung injury lung fibrosis NLRP3 IL-17 TGF-β<sub>1</sub> |
| title | Diesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β<sub>1</sub>/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in Mice |
| title_full | Diesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β<sub>1</sub>/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in Mice |
| title_fullStr | Diesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β<sub>1</sub>/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in Mice |
| title_full_unstemmed | Diesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β<sub>1</sub>/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in Mice |
| title_short | Diesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β<sub>1</sub>/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in Mice |
| title_sort | diesel exhaust particulates enhances susceptibility of lps induced acute lung injury through upregulation of the il 17 cytokine derived tgf β sub 1 sub collagen i expression and activation of nlrp3 inflammasome signaling in mice |
| topic | diesel exhaust particulate acute lung injury lung fibrosis NLRP3 IL-17 TGF-β<sub>1</sub> |
| url | https://www.mdpi.com/2218-273X/11/1/67 |
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