Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM?
The ISAGA immunocapture test for the detection of anti-Toxoplasma immunoglobulin M is a manual technique known for its excellent sensitivity and specificity. The purpose of this retrospective, multicenter study was to compare the performances and agreement between ISAGA and other IgM detection techn...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | Parasite |
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| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite230163/parasite230163.html |
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| author | Deleplancque Anne-Sophie Fricker-Hidalgo Hélène Pomares Christelle L’Ollivier Coralie Lemoine Jean-Philippe Cimon Bernard Paris Luc Houzé Sandrine Villena Isabelle Pelloux Hervé Villard Odile |
| author_facet | Deleplancque Anne-Sophie Fricker-Hidalgo Hélène Pomares Christelle L’Ollivier Coralie Lemoine Jean-Philippe Cimon Bernard Paris Luc Houzé Sandrine Villena Isabelle Pelloux Hervé Villard Odile |
| author_sort | Deleplancque Anne-Sophie |
| collection | DOAJ |
| description | The ISAGA immunocapture test for the detection of anti-Toxoplasma immunoglobulin M is a manual technique known for its excellent sensitivity and specificity. The purpose of this retrospective, multicenter study was to compare the performances and agreement between ISAGA and other IgM detection techniques before cessation of ISAGA production. The analytic performance of the different tests was evaluated using 1,341 serum samples from adults with positive IgM and negative IgG to Toxoplasma gondii, and 1,206 sera from neonates born to mothers with seroconversion. The agreement between the tests was evaluated on 13,506 adult and 5,795 child serum samples. The sensitivity of Toxo-ISAGA IgM® (adults 98.7%, neonates 63.1%) was similar to that of Platelia Toxo IgM® (adults 94.4%, neonates 64.6%), and significantly higher than Liaison Toxo IgM® (adults 90.6%), Architect/Alinity Toxo IgM® (adults 95.7%, neonates 48.6%), and Vidas Toxo IgM® (adults 81.8%, neonates 17.5%). However, the specificities varied between 24.4% (Platelia Toxo IgM®) and 95.2% (Liaison Toxo IgM®) in adults and were >95% for all tests in neonates. An analysis of the kappa coefficients showed better agreement between ISAGA IgM® and the other tests in children (0.75–0.83%) than in adults (0.11–0.53%). We conclude that, in the absence of Toxo-ISAGA IgM®, the association of a very sensitive technique (Platelia Toxo IgM® or Architect/Alinity Toxo IgM®) and a very specific technique (Vidas Toxo IgM® or Liaison Toxo IgM®) is recommended for IgM detection in adult sera. For neonates, Platelia Toxo IgM® appeared to be the best alternative to replace Toxo-ISAGA IgM®. |
| first_indexed | 2024-03-08T03:06:38Z |
| format | Article |
| id | doaj.art-23a28f3e724d498181974c23a478caf8 |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-08T03:06:38Z |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-23a28f3e724d498181974c23a478caf82024-02-13T08:35:26ZengEDP SciencesParasite1776-10422024-01-0131710.1051/parasite/2024004parasite230163Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM?Deleplancque Anne-Sophie0Fricker-Hidalgo Hélène1Pomares Christelle2L’Ollivier Coralie3Lemoine Jean-Philippe4Cimon Bernard5Paris Luc6Houzé Sandrine7Villena Isabelle8Pelloux Hervé9Villard Odile10Centre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgCentre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgCentre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgCentre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgLaboratoire de Parasitologie-Mycologie, CHU d’AngersCentre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgCentre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgCentre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgCentre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgCentre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgCentre National de Référence Toxoplasmose – Pôle Sérologie, Hôpitaux Universitaires de StrasbourgThe ISAGA immunocapture test for the detection of anti-Toxoplasma immunoglobulin M is a manual technique known for its excellent sensitivity and specificity. The purpose of this retrospective, multicenter study was to compare the performances and agreement between ISAGA and other IgM detection techniques before cessation of ISAGA production. The analytic performance of the different tests was evaluated using 1,341 serum samples from adults with positive IgM and negative IgG to Toxoplasma gondii, and 1,206 sera from neonates born to mothers with seroconversion. The agreement between the tests was evaluated on 13,506 adult and 5,795 child serum samples. The sensitivity of Toxo-ISAGA IgM® (adults 98.7%, neonates 63.1%) was similar to that of Platelia Toxo IgM® (adults 94.4%, neonates 64.6%), and significantly higher than Liaison Toxo IgM® (adults 90.6%), Architect/Alinity Toxo IgM® (adults 95.7%, neonates 48.6%), and Vidas Toxo IgM® (adults 81.8%, neonates 17.5%). However, the specificities varied between 24.4% (Platelia Toxo IgM®) and 95.2% (Liaison Toxo IgM®) in adults and were >95% for all tests in neonates. An analysis of the kappa coefficients showed better agreement between ISAGA IgM® and the other tests in children (0.75–0.83%) than in adults (0.11–0.53%). We conclude that, in the absence of Toxo-ISAGA IgM®, the association of a very sensitive technique (Platelia Toxo IgM® or Architect/Alinity Toxo IgM®) and a very specific technique (Vidas Toxo IgM® or Liaison Toxo IgM®) is recommended for IgM detection in adult sera. For neonates, Platelia Toxo IgM® appeared to be the best alternative to replace Toxo-ISAGA IgM®.https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite230163/parasite230163.htmltoxoplasmosisigm detectionsensitivityspecificityisaga igm® |
| spellingShingle | Deleplancque Anne-Sophie Fricker-Hidalgo Hélène Pomares Christelle L’Ollivier Coralie Lemoine Jean-Philippe Cimon Bernard Paris Luc Houzé Sandrine Villena Isabelle Pelloux Hervé Villard Odile Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM? Parasite toxoplasmosis igm detection sensitivity specificity isaga igm® |
| title | Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM? |
| title_full | Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM? |
| title_fullStr | Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM? |
| title_full_unstemmed | Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM? |
| title_short | Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM? |
| title_sort | comparative performance of isaga igm and elisa assays for the diagnosis of maternal and congenital toxoplasma infections which technique could replace isaga igm |
| topic | toxoplasmosis igm detection sensitivity specificity isaga igm® |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite230163/parasite230163.html |
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