Effect of probiotic supplementation on growth and global gene expression in dairy cows
Use of probiotic supplements as a non-chemical approach to promote health has increased in animal production. The present study evaluated the effect of oral probiotic administration on growth and global gene expression profile in dairy cows. Lactating Holstein-Friesian cows received a daily dose (50...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09712119.2017.1292913 |
| _version_ | 1818551707285585920 |
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| author | Sarah Adjei-Fremah Kingsley Ekwemalor Emmanuel K. Asiamah Hamid Ismail Salam Ibrahim Mulumebet Worku |
| author_facet | Sarah Adjei-Fremah Kingsley Ekwemalor Emmanuel K. Asiamah Hamid Ismail Salam Ibrahim Mulumebet Worku |
| author_sort | Sarah Adjei-Fremah |
| collection | DOAJ |
| description | Use of probiotic supplements as a non-chemical approach to promote health has increased in animal production. The present study evaluated the effect of oral probiotic administration on growth and global gene expression profile in dairy cows. Lactating Holstein-Friesian cows received a daily dose (50 ml) of a commercial probiotic (containing Lactobacillus acidophilus, Saccharomyces cerevisiae, Enterococcus faecium, Aspergillus oryza and Bacillus subtilis) for 60 days. A microarray experiment was performed with blood collected at day-0 and day-60. Although probiotic supplementation had no effect on body weight, PCV and total protein concentration in plasma (P > 0.05), per cent lymphocyte count increased (P < 0.05), and per cent neutrophil count decreased (P < 0.05) in probiotic-treated animals. Gene expression analysis identified 10,859 differentially expressed genes, 1168 up-regulated and 9691 down-regulated genes, respectively, following probiotic treatment. Single experiment pathway analysis identified 87 bovine pathways impacted by probiotic treatment. These pathways included the Toll-like receptor (TLR), inflammation response and Wingless signalling pathways. Oral administration of probiotics to dairy cows had a systemic effect on global gene expression, such as on genes involved in immunity and homeostasis. The results of this study show that the utilization of probiotics in animal agriculture impacts genes important to dairy cow health and production. |
| first_indexed | 2024-12-12T09:03:29Z |
| format | Article |
| id | doaj.art-23a295d0c6384911b276e4742a23eaa4 |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-12-12T09:03:29Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-23a295d0c6384911b276e4742a23eaa42022-12-22T00:29:45ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442018-01-0146125726310.1080/09712119.2017.12929131292913Effect of probiotic supplementation on growth and global gene expression in dairy cowsSarah Adjei-Fremah0Kingsley Ekwemalor1Emmanuel K. Asiamah2Hamid Ismail3Salam Ibrahim4Mulumebet Worku5North Carolina Agricultural and Technical State UniversityNorth Carolina Agricultural and Technical State UniversityNorth Carolina Agricultural and Technical State UniversityNorth Carolina Agricultural and Technical State UniversityNorth Carolina Agricultural and Technical State UniversityNorth Carolina Agricultural and Technical State UniversityUse of probiotic supplements as a non-chemical approach to promote health has increased in animal production. The present study evaluated the effect of oral probiotic administration on growth and global gene expression profile in dairy cows. Lactating Holstein-Friesian cows received a daily dose (50 ml) of a commercial probiotic (containing Lactobacillus acidophilus, Saccharomyces cerevisiae, Enterococcus faecium, Aspergillus oryza and Bacillus subtilis) for 60 days. A microarray experiment was performed with blood collected at day-0 and day-60. Although probiotic supplementation had no effect on body weight, PCV and total protein concentration in plasma (P > 0.05), per cent lymphocyte count increased (P < 0.05), and per cent neutrophil count decreased (P < 0.05) in probiotic-treated animals. Gene expression analysis identified 10,859 differentially expressed genes, 1168 up-regulated and 9691 down-regulated genes, respectively, following probiotic treatment. Single experiment pathway analysis identified 87 bovine pathways impacted by probiotic treatment. These pathways included the Toll-like receptor (TLR), inflammation response and Wingless signalling pathways. Oral administration of probiotics to dairy cows had a systemic effect on global gene expression, such as on genes involved in immunity and homeostasis. The results of this study show that the utilization of probiotics in animal agriculture impacts genes important to dairy cow health and production.http://dx.doi.org/10.1080/09712119.2017.1292913Probioticmicroarrayinnate immunityWnt signallingHolstein-Friesiantoll-like receptors |
| spellingShingle | Sarah Adjei-Fremah Kingsley Ekwemalor Emmanuel K. Asiamah Hamid Ismail Salam Ibrahim Mulumebet Worku Effect of probiotic supplementation on growth and global gene expression in dairy cows Journal of Applied Animal Research Probiotic microarray innate immunity Wnt signalling Holstein-Friesian toll-like receptors |
| title | Effect of probiotic supplementation on growth and global gene expression in dairy cows |
| title_full | Effect of probiotic supplementation on growth and global gene expression in dairy cows |
| title_fullStr | Effect of probiotic supplementation on growth and global gene expression in dairy cows |
| title_full_unstemmed | Effect of probiotic supplementation on growth and global gene expression in dairy cows |
| title_short | Effect of probiotic supplementation on growth and global gene expression in dairy cows |
| title_sort | effect of probiotic supplementation on growth and global gene expression in dairy cows |
| topic | Probiotic microarray innate immunity Wnt signalling Holstein-Friesian toll-like receptors |
| url | http://dx.doi.org/10.1080/09712119.2017.1292913 |
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