Effects of Two Kinds of Extracts of <i>Cistanche deserticola</i> on Intestinal Microbiota and Its Metabolism
<i>Cistanche deserticola</i> belongs to the Liedang family. Known as “desert ginseng”, it has high medicinal value and plays important roles in endocrine regulation, neuroprotection, immune regulation, and other processes. Some studies have shown that single substances such as polysaccha...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/18/2897 |
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| author | Yilin Li Yalin Zhang Xiaoming Su Pengfei Zou Xinyang Wang Jie Chen Xuan Zhu |
| author_facet | Yilin Li Yalin Zhang Xiaoming Su Pengfei Zou Xinyang Wang Jie Chen Xuan Zhu |
| author_sort | Yilin Li |
| collection | DOAJ |
| description | <i>Cistanche deserticola</i> belongs to the Liedang family. Known as “desert ginseng”, it has high medicinal value and plays important roles in endocrine regulation, neuroprotection, immune regulation, and other processes. Some studies have shown that single substances such as polysaccharides and phenylethanolside can affect intestinal microbiota, but few studies have studied the synergistic effect of various components in <i>Cistanche deserticola</i> extracts on intestinal microbiota. Therefore, in this study, through an in vitro digestion model (Changdao Moni, CDMN) combined with 16S rRNA gene amplification sequencing technology and untargeted metabolomics technology, it was found that the two extracts all had significant effects on the enteric cavity and mucosal flora. Both extracts inhibited <i>Bacteroides</i> in the intestinal cavity and <i>Parabacteroides</i> and <i>Ruminococcus 2</i> in the intestinal mucosa and promoted <i>Bifidobacterium</i> and <i>Prevotella</i> in the intestinal cavity and <i>Megasphaera</i> in the intestinal mucosa. The aqueous extract also inhibited <i>Phascolarctobacterium</i>. Both extracts also significantly increased the production of short-chain fatty acids, especially butyrate. The intake of extract had significant effects on the metabolic pathways related to amino acids and lipids. Indoles were upregulated by the aqueous extract but downregulated by the alcohol extract. In addition, the extract also had a significant effect on the hemolytic phosphorus esters. In conclusion, the two kinds of extracts have different effects on intestinal microbiota and its metabolism. This study provides guiding significance for the edibility and food development of <i>Cistanche deserticola</i>. |
| first_indexed | 2024-03-10T00:02:04Z |
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| id | doaj.art-23a2cf8dc73f4be28c114119962dc36e |
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| language | English |
| last_indexed | 2024-03-10T00:02:04Z |
| publishDate | 2022-09-01 |
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| series | Foods |
| spelling | doaj.art-23a2cf8dc73f4be28c114119962dc36e2023-11-23T16:15:03ZengMDPI AGFoods2304-81582022-09-011118289710.3390/foods11182897Effects of Two Kinds of Extracts of <i>Cistanche deserticola</i> on Intestinal Microbiota and Its MetabolismYilin Li0Yalin Zhang1Xiaoming Su2Pengfei Zou3Xinyang Wang4Jie Chen5Xuan Zhu6Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Jimei University, Xiamen 361021, ChinaSchool of Food Science and Bioengineering, Zhejiang Gongshang University, Hangzhou 310018, ChinaWeifang Elbe Health Food Co., Ltd., Weifang 261057, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Jimei University, Xiamen 361021, ChinaWeifang Elbe Health Food Co., Ltd., Weifang 261057, ChinaSchool of Food Science and Bioengineering, Zhejiang Gongshang University, Hangzhou 310018, ChinaSchool of Food Science and Bioengineering, Zhejiang Gongshang University, Hangzhou 310018, China<i>Cistanche deserticola</i> belongs to the Liedang family. Known as “desert ginseng”, it has high medicinal value and plays important roles in endocrine regulation, neuroprotection, immune regulation, and other processes. Some studies have shown that single substances such as polysaccharides and phenylethanolside can affect intestinal microbiota, but few studies have studied the synergistic effect of various components in <i>Cistanche deserticola</i> extracts on intestinal microbiota. Therefore, in this study, through an in vitro digestion model (Changdao Moni, CDMN) combined with 16S rRNA gene amplification sequencing technology and untargeted metabolomics technology, it was found that the two extracts all had significant effects on the enteric cavity and mucosal flora. Both extracts inhibited <i>Bacteroides</i> in the intestinal cavity and <i>Parabacteroides</i> and <i>Ruminococcus 2</i> in the intestinal mucosa and promoted <i>Bifidobacterium</i> and <i>Prevotella</i> in the intestinal cavity and <i>Megasphaera</i> in the intestinal mucosa. The aqueous extract also inhibited <i>Phascolarctobacterium</i>. Both extracts also significantly increased the production of short-chain fatty acids, especially butyrate. The intake of extract had significant effects on the metabolic pathways related to amino acids and lipids. Indoles were upregulated by the aqueous extract but downregulated by the alcohol extract. In addition, the extract also had a significant effect on the hemolytic phosphorus esters. In conclusion, the two kinds of extracts have different effects on intestinal microbiota and its metabolism. This study provides guiding significance for the edibility and food development of <i>Cistanche deserticola</i>.https://www.mdpi.com/2304-8158/11/18/2897<i>Cistanche deserticola</i>alcohol extractionaqueous extractiongut microbiotauntargeted metabolomics |
| spellingShingle | Yilin Li Yalin Zhang Xiaoming Su Pengfei Zou Xinyang Wang Jie Chen Xuan Zhu Effects of Two Kinds of Extracts of <i>Cistanche deserticola</i> on Intestinal Microbiota and Its Metabolism Foods <i>Cistanche deserticola</i> alcohol extraction aqueous extraction gut microbiota untargeted metabolomics |
| title | Effects of Two Kinds of Extracts of <i>Cistanche deserticola</i> on Intestinal Microbiota and Its Metabolism |
| title_full | Effects of Two Kinds of Extracts of <i>Cistanche deserticola</i> on Intestinal Microbiota and Its Metabolism |
| title_fullStr | Effects of Two Kinds of Extracts of <i>Cistanche deserticola</i> on Intestinal Microbiota and Its Metabolism |
| title_full_unstemmed | Effects of Two Kinds of Extracts of <i>Cistanche deserticola</i> on Intestinal Microbiota and Its Metabolism |
| title_short | Effects of Two Kinds of Extracts of <i>Cistanche deserticola</i> on Intestinal Microbiota and Its Metabolism |
| title_sort | effects of two kinds of extracts of i cistanche deserticola i on intestinal microbiota and its metabolism |
| topic | <i>Cistanche deserticola</i> alcohol extraction aqueous extraction gut microbiota untargeted metabolomics |
| url | https://www.mdpi.com/2304-8158/11/18/2897 |
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