Identification of IncA Plasmid, Harboring <i>bla</i><sub>VIM-1</sub> Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized Patient
In the present study, we analyzed the genome of two <i>S. enterica</i> strains TS1 and TS2 from stool and blood cultures, respectively, and one strain of <i>C. freundii</i> TS3, isolated from a single hospitalized patient with acute myeloid leukemia. The <i>S. enterica&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/12/1659 |
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| author | Alessandra Piccirilli Sascia Di Marcantonio Venera Costantino Omar Simonetti Marina Busetti Roberto Luzzati Luigi Principe Marco Di Domenico Antonio Rinaldi Cesare Cammà Mariagrazia Perilli |
| author_facet | Alessandra Piccirilli Sascia Di Marcantonio Venera Costantino Omar Simonetti Marina Busetti Roberto Luzzati Luigi Principe Marco Di Domenico Antonio Rinaldi Cesare Cammà Mariagrazia Perilli |
| author_sort | Alessandra Piccirilli |
| collection | DOAJ |
| description | In the present study, we analyzed the genome of two <i>S. enterica</i> strains TS1 and TS2 from stool and blood cultures, respectively, and one strain of <i>C. freundii</i> TS3, isolated from a single hospitalized patient with acute myeloid leukemia. The <i>S. enterica</i> Goldcoast ST358 (O:8 (C2-C3) serogroup), sequenced by the MiSeq Illumina system, showed the presence of β-lactamase genes (<i>bla</i><sub>VIM-1</sub>, <i>bla</i><sub>SHV-12</sub> and <i>bla</i><sub>OXA-10</sub>), <i>aadA1</i>, <i>ant(2″)-Ia</i>, <i>aac(6′)-Iaa</i>, <i>aac(6′)-Ib3</i>, <i>aac(6′)-Ib-cr</i>, <i>qnrVC6</i>, <i>parC(T57S),</i> and several incompatibility plasmids. A wide variety of insertion sequences (ISs) and transposon elements were identified. In <i>C. freundii</i> TS3, these were the <i>bla</i><sub>VIM-1</sub>, <i>bla</i><sub>CMY-150</sub>, and <i>bla</i><sub>SHV-12</sub>, <i>aadA1</i>, <i>aac(6′)-Ib3</i>, <i>aac(6′)-Ib-cr</i>, <i>mph(A)</i>, <i>sul1</i>, <i>dfrA14</i>, <i>ARR-2</i>, <i>qnrVC6</i>, and <i>qnrB38</i>. IncA plasmid isolated from <i>E.coli</i>/K12 transconjugant and <i>C. freundii</i> exhibited a sequence identity >99.9%. The transfer of IncA plasmid was evaluated by conjugation experiments. |
| first_indexed | 2024-03-08T21:03:17Z |
| format | Article |
| id | doaj.art-23a351fa9439491f94a8efc7c50fd5e6 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-08T21:03:17Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-23a351fa9439491f94a8efc7c50fd5e62023-12-22T13:47:48ZengMDPI AGAntibiotics2079-63822023-11-011212165910.3390/antibiotics12121659Identification of IncA Plasmid, Harboring <i>bla</i><sub>VIM-1</sub> Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized PatientAlessandra Piccirilli0Sascia Di Marcantonio1Venera Costantino2Omar Simonetti3Marina Busetti4Roberto Luzzati5Luigi Principe6Marco Di Domenico7Antonio Rinaldi8Cesare Cammà9Mariagrazia Perilli10Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, ItalyDepartment of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, ItalyMicrobiology Unit, Trieste University Hospital (ASUGI), 34125 Trieste, ItalyInfectious Diseases Unit, Trieste University Hospital (ASUGI), 34125 Trieste, ItalyMicrobiology Unit, Trieste University Hospital (ASUGI), 34125 Trieste, ItalyInfectious Diseases Unit, Trieste University Hospital (ASUGI), 34125 Trieste, ItalyClinical Pathology and Microbiology Unit, “S. Giovanni di Dio” Hospital, 88900 Crotone, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Campo Boario, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Campo Boario, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Campo Boario, 64100 Teramo, ItalyDepartment of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, ItalyIn the present study, we analyzed the genome of two <i>S. enterica</i> strains TS1 and TS2 from stool and blood cultures, respectively, and one strain of <i>C. freundii</i> TS3, isolated from a single hospitalized patient with acute myeloid leukemia. The <i>S. enterica</i> Goldcoast ST358 (O:8 (C2-C3) serogroup), sequenced by the MiSeq Illumina system, showed the presence of β-lactamase genes (<i>bla</i><sub>VIM-1</sub>, <i>bla</i><sub>SHV-12</sub> and <i>bla</i><sub>OXA-10</sub>), <i>aadA1</i>, <i>ant(2″)-Ia</i>, <i>aac(6′)-Iaa</i>, <i>aac(6′)-Ib3</i>, <i>aac(6′)-Ib-cr</i>, <i>qnrVC6</i>, <i>parC(T57S),</i> and several incompatibility plasmids. A wide variety of insertion sequences (ISs) and transposon elements were identified. In <i>C. freundii</i> TS3, these were the <i>bla</i><sub>VIM-1</sub>, <i>bla</i><sub>CMY-150</sub>, and <i>bla</i><sub>SHV-12</sub>, <i>aadA1</i>, <i>aac(6′)-Ib3</i>, <i>aac(6′)-Ib-cr</i>, <i>mph(A)</i>, <i>sul1</i>, <i>dfrA14</i>, <i>ARR-2</i>, <i>qnrVC6</i>, and <i>qnrB38</i>. IncA plasmid isolated from <i>E.coli</i>/K12 transconjugant and <i>C. freundii</i> exhibited a sequence identity >99.9%. The transfer of IncA plasmid was evaluated by conjugation experiments.https://www.mdpi.com/2079-6382/12/12/1659<i>Salmonella enterica</i> Goldcoast<i>C. freundii</i>WGSVIMIncAβ-lactamase |
| spellingShingle | Alessandra Piccirilli Sascia Di Marcantonio Venera Costantino Omar Simonetti Marina Busetti Roberto Luzzati Luigi Principe Marco Di Domenico Antonio Rinaldi Cesare Cammà Mariagrazia Perilli Identification of IncA Plasmid, Harboring <i>bla</i><sub>VIM-1</sub> Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized Patient Antibiotics <i>Salmonella enterica</i> Goldcoast <i>C. freundii</i> WGS VIM IncA β-lactamase |
| title | Identification of IncA Plasmid, Harboring <i>bla</i><sub>VIM-1</sub> Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized Patient |
| title_full | Identification of IncA Plasmid, Harboring <i>bla</i><sub>VIM-1</sub> Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized Patient |
| title_fullStr | Identification of IncA Plasmid, Harboring <i>bla</i><sub>VIM-1</sub> Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized Patient |
| title_full_unstemmed | Identification of IncA Plasmid, Harboring <i>bla</i><sub>VIM-1</sub> Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized Patient |
| title_short | Identification of IncA Plasmid, Harboring <i>bla</i><sub>VIM-1</sub> Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized Patient |
| title_sort | identification of inca plasmid harboring i bla i sub vim 1 sub gene in i s enterica i goldcoast st358 and i c freundii i st62 isolated in a hospitalized patient |
| topic | <i>Salmonella enterica</i> Goldcoast <i>C. freundii</i> WGS VIM IncA β-lactamase |
| url | https://www.mdpi.com/2079-6382/12/12/1659 |
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