Contribution of the Nucleus Cuneiformis to the Antinociceptive Effects of Systemic Morphine on Inflammatory Pain in Rats
Introduction: The role of midbrain reticular formation, which includes the nucleus cuneiformis (NCF), as a crucial antinociceptive region in descending pain modulation has long been investigated. In this study, we tried to highlight the role of NCF in morphine-induced antinociception in formalin-ind...
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2011-10-01
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| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-89&slc_lang=en&sid=1 |
| _version_ | 1827334499201974272 |
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| author | Abdolaziz Ronaghi Mohammad Ebrahimzadeh Abbas Haghparast |
| author_facet | Abdolaziz Ronaghi Mohammad Ebrahimzadeh Abbas Haghparast |
| author_sort | Abdolaziz Ronaghi |
| collection | DOAJ |
| description | Introduction: The role of midbrain reticular formation, which includes the nucleus cuneiformis (NCF), as a crucial antinociceptive region in descending pain modulation has long been investigated. In this study, we tried to highlight the role of NCF in morphine-induced antinociception in formalin-induced pain model in rats. Methods: A total of 201 male Wistar rats weighing 260-310 g were used in this study. The effective dose of morphine in systemic administration (intraperitoneal i.p.) was determined after a dose- and time-response protocol. In consequent groups, bilateral electrolytic lesion (500 μA, 30 sec) or reversible inactivation (lidocaine 2%) were used in the NCF before systemic administration of morphine, and then, the nociceptive test was immediately carried out. Results: The results showed that administration of 6 mg/kg morphine, 30 min before the formalin test, is the best dose- and time-response set in these experiments. The obtained data also indicated that bilateral electrical destruction or reversible inactivation of the NCF significantly decreased antinociceptive responses of systemic morphine (6 mg/kg i.p.) during the second phase of formalin test (P<0.05). Discussion: Therefore, it seems that opioid receptors located in the NCF may be involved in modulation of central sensitization which occurred in inflammatory pain in rats. |
| first_indexed | 2024-03-07T17:55:34Z |
| format | Article |
| id | doaj.art-23a66a5d232d4f919f53a47793c53396 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:55:34Z |
| publishDate | 2011-10-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-23a66a5d232d4f919f53a47793c533962024-03-02T12:15:54ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422011-10-01313543Contribution of the Nucleus Cuneiformis to the Antinociceptive Effects of Systemic Morphine on Inflammatory Pain in RatsAbdolaziz Ronaghi0Mohammad Ebrahimzadeh1Abbas Haghparast2 Introduction: The role of midbrain reticular formation, which includes the nucleus cuneiformis (NCF), as a crucial antinociceptive region in descending pain modulation has long been investigated. In this study, we tried to highlight the role of NCF in morphine-induced antinociception in formalin-induced pain model in rats. Methods: A total of 201 male Wistar rats weighing 260-310 g were used in this study. The effective dose of morphine in systemic administration (intraperitoneal i.p.) was determined after a dose- and time-response protocol. In consequent groups, bilateral electrolytic lesion (500 μA, 30 sec) or reversible inactivation (lidocaine 2%) were used in the NCF before systemic administration of morphine, and then, the nociceptive test was immediately carried out. Results: The results showed that administration of 6 mg/kg morphine, 30 min before the formalin test, is the best dose- and time-response set in these experiments. The obtained data also indicated that bilateral electrical destruction or reversible inactivation of the NCF significantly decreased antinociceptive responses of systemic morphine (6 mg/kg i.p.) during the second phase of formalin test (P<0.05). Discussion: Therefore, it seems that opioid receptors located in the NCF may be involved in modulation of central sensitization which occurred in inflammatory pain in rats.http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-89&slc_lang=en&sid=1Nucleus CuneiformisElectrolytic LesionReversible InactivationMorphineFormalin TestRat. |
| spellingShingle | Abdolaziz Ronaghi Mohammad Ebrahimzadeh Abbas Haghparast Contribution of the Nucleus Cuneiformis to the Antinociceptive Effects of Systemic Morphine on Inflammatory Pain in Rats Basic and Clinical Neuroscience Nucleus Cuneiformis Electrolytic Lesion Reversible Inactivation Morphine Formalin Test Rat. |
| title | Contribution of the Nucleus Cuneiformis to the Antinociceptive Effects of Systemic Morphine on Inflammatory Pain in Rats |
| title_full | Contribution of the Nucleus Cuneiformis to the Antinociceptive Effects of Systemic Morphine on Inflammatory Pain in Rats |
| title_fullStr | Contribution of the Nucleus Cuneiformis to the Antinociceptive Effects of Systemic Morphine on Inflammatory Pain in Rats |
| title_full_unstemmed | Contribution of the Nucleus Cuneiformis to the Antinociceptive Effects of Systemic Morphine on Inflammatory Pain in Rats |
| title_short | Contribution of the Nucleus Cuneiformis to the Antinociceptive Effects of Systemic Morphine on Inflammatory Pain in Rats |
| title_sort | contribution of the nucleus cuneiformis to the antinociceptive effects of systemic morphine on inflammatory pain in rats |
| topic | Nucleus Cuneiformis Electrolytic Lesion Reversible Inactivation Morphine Formalin Test Rat. |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-89&slc_lang=en&sid=1 |
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