Phenotypic features and molecular study of airborne Penicillium species isolated in the northern part of the Persian Gulf, Bushehr, Iran
Background and Purpose: The main environmental saprobes, such as Penicillium, play an essential role in natural ecosystems as economically, ecologically, and medically important microorganisms. Biodiversity of this genus has not been described in Bushehr city, Iran. The present study is based on air...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2021-06-01
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| Series: | Current Medical Mycology |
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| Online Access: | http://cmm.mazums.ac.ir/article_135951_54f1f17177553a2f76876b8ea43a0fe9.pdf |
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| author | Behrouz Naeimi Iman Mohsenifard saham ansari Farzaneh Sadeghzadeh Gholamreza Khamisipour Sina Dobaradaran Fatemeh faraji ghasemi Bahram Ahmadi |
| author_facet | Behrouz Naeimi Iman Mohsenifard saham ansari Farzaneh Sadeghzadeh Gholamreza Khamisipour Sina Dobaradaran Fatemeh faraji ghasemi Bahram Ahmadi |
| author_sort | Behrouz Naeimi |
| collection | DOAJ |
| description | Background and Purpose: The main environmental saprobes, such as Penicillium, play an essential role in natural ecosystems as economically, ecologically, and medically important microorganisms. Biodiversity of this genus has not been described in Bushehr city, Iran. The present study is based on air biodiversity of Penicillium species on culture dependent approach and culture-independent technique using partial b-tubulin sequences.Materials and Methods: By using active sampling with a high volume air sampler, a total of 157 Penicillium isolates were selected and screened for phenotypic characters. For the purposes of the study, 46 strains representative of 11 morphological species wereselected and identified by molecular analysis.Results: Based on the findings, P. crustosum (18 isolates, 39.1%) and P. chrysogenum (15 isolates, 32.6%) were the most common isolated species, followed by P. brevicompactum, P. rubens, P. citrinum, P. italicum (each 2 isolates, 4.3%), P. olsonii, P. expansum, P. griseofulvum, P. palitans, and P. polonicum (each 1 isolate, 2.1%).Except for P. chrysogenum and P. expansum with floccose colony texture, the rest of the isolated species had velutinous texture.Conclusion: This is the first report in southern Iran to identify a large number of Penicillium strains isolated from air samples, showing P. crustosum and P. chrysogenum as the most common isolated species. |
| first_indexed | 2024-04-11T04:10:27Z |
| format | Article |
| id | doaj.art-23a822bd9a184840a84b96f229efe791 |
| institution | Directory Open Access Journal |
| issn | 2423-3439 2423-3420 |
| language | English |
| last_indexed | 2024-04-11T04:10:27Z |
| publishDate | 2021-06-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Current Medical Mycology |
| spelling | doaj.art-23a822bd9a184840a84b96f229efe7912023-01-01T06:31:01ZengMazandaran University of Medical SciencesCurrent Medical Mycology2423-34392423-34202021-06-0172222810.18502/cmm.7.2.7035135951Phenotypic features and molecular study of airborne Penicillium species isolated in the northern part of the Persian Gulf, Bushehr, IranBehrouz Naeimi0Iman Mohsenifard1saham ansari2Farzaneh Sadeghzadeh3Gholamreza Khamisipour4Sina Dobaradaran5Fatemeh faraji ghasemi6Bahram Ahmadi7Department of Medical Laboratory Sciences, Faculty of Paramedical, Bushehr University of Medical Sciences, Bushehr, IranMedical Laboratory Sciences Student, Student Research Committee, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Laboratory Sciences, Faculty of Paramedical, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Hematology, Faculty of Allied Medicine, Bushehr University of Medical Sciences, Bushehr, IranSystems Environmental Health and Energy Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, IranSystems Environmental Health and Energy Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Medical Laboratory Sciences, Faculty of Paramedical, Bushehr University of Medical Sciences, Bushehr, IranBackground and Purpose: The main environmental saprobes, such as Penicillium, play an essential role in natural ecosystems as economically, ecologically, and medically important microorganisms. Biodiversity of this genus has not been described in Bushehr city, Iran. The present study is based on air biodiversity of Penicillium species on culture dependent approach and culture-independent technique using partial b-tubulin sequences.Materials and Methods: By using active sampling with a high volume air sampler, a total of 157 Penicillium isolates were selected and screened for phenotypic characters. For the purposes of the study, 46 strains representative of 11 morphological species wereselected and identified by molecular analysis.Results: Based on the findings, P. crustosum (18 isolates, 39.1%) and P. chrysogenum (15 isolates, 32.6%) were the most common isolated species, followed by P. brevicompactum, P. rubens, P. citrinum, P. italicum (each 2 isolates, 4.3%), P. olsonii, P. expansum, P. griseofulvum, P. palitans, and P. polonicum (each 1 isolate, 2.1%).Except for P. chrysogenum and P. expansum with floccose colony texture, the rest of the isolated species had velutinous texture.Conclusion: This is the first report in southern Iran to identify a large number of Penicillium strains isolated from air samples, showing P. crustosum and P. chrysogenum as the most common isolated species.http://cmm.mazums.ac.ir/article_135951_54f1f17177553a2f76876b8ea43a0fe9.pdfbeta-tubulinbushehrpenicillium |
| spellingShingle | Behrouz Naeimi Iman Mohsenifard saham ansari Farzaneh Sadeghzadeh Gholamreza Khamisipour Sina Dobaradaran Fatemeh faraji ghasemi Bahram Ahmadi Phenotypic features and molecular study of airborne Penicillium species isolated in the northern part of the Persian Gulf, Bushehr, Iran Current Medical Mycology beta-tubulin bushehr penicillium |
| title | Phenotypic features and molecular study of airborne Penicillium species isolated in the northern part of the Persian Gulf, Bushehr, Iran |
| title_full | Phenotypic features and molecular study of airborne Penicillium species isolated in the northern part of the Persian Gulf, Bushehr, Iran |
| title_fullStr | Phenotypic features and molecular study of airborne Penicillium species isolated in the northern part of the Persian Gulf, Bushehr, Iran |
| title_full_unstemmed | Phenotypic features and molecular study of airborne Penicillium species isolated in the northern part of the Persian Gulf, Bushehr, Iran |
| title_short | Phenotypic features and molecular study of airborne Penicillium species isolated in the northern part of the Persian Gulf, Bushehr, Iran |
| title_sort | phenotypic features and molecular study of airborne penicillium species isolated in the northern part of the persian gulf bushehr iran |
| topic | beta-tubulin bushehr penicillium |
| url | http://cmm.mazums.ac.ir/article_135951_54f1f17177553a2f76876b8ea43a0fe9.pdf |
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