Evaluation of the anti-infective potential of the seed endophytic fungi of Corchorus olitorius through metabolomics and molecular docking approach
Abstract Background Endophytic fungi are very rich sources of natural antibacterial and antifungal compounds. The main aim of this study is to isolate the fungal endophytes from the medicinal plant Corchorus olitorius seeds (F. Malvaceae), followed by antimicrobial screening against various bacteria...
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| Format: | Article |
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BMC
2023-11-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-03092-5 |
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| author | Arwa Mortada Ahmed Ayman M. Ibrahim Ramadan Yahia Nourhan Hisham Shady Basma Khalaf Mahmoud Usama Ramadan Abdelmohsen Mostafa A. Fouad |
| author_facet | Arwa Mortada Ahmed Ayman M. Ibrahim Ramadan Yahia Nourhan Hisham Shady Basma Khalaf Mahmoud Usama Ramadan Abdelmohsen Mostafa A. Fouad |
| author_sort | Arwa Mortada Ahmed |
| collection | DOAJ |
| description | Abstract Background Endophytic fungi are very rich sources of natural antibacterial and antifungal compounds. The main aim of this study is to isolate the fungal endophytes from the medicinal plant Corchorus olitorius seeds (F. Malvaceae), followed by antimicrobial screening against various bacterial and fungal strains. Results Seven endophytic fungal strains belonging to different three genera were isolated, including Penicillium, Fusarium, and Aspergillus. The seven isolated endophytic strains revealed selective noticeable activity against Escherichia coli (ATCC25922) with varied IC50s ranging from 1.19 to 10 µg /mL, in which Aspergillus sp. (Ar 6) exhibited the strongest potency against E. coli (ATCC 25,922) and candida albicans (ATCC 10,231) with IC50s 1.19 and 15 µg /mL, respectively. Therefore, the chemical profiling of Aspergillus sp. (Ar 6) crude extract was performed using LC-HR-ESI-MS and led to the dereplication of sixteen compounds of various classes (1–16). In-silico analysis of the dereplicated metabolites led to highlighting the compounds responsible for the antimicrobial activity of Aspergillus sp. extract. Moreover, molecular docking showed the potential targets of the metabolites; Astellatol (5), Aspergillipeptide A (10), and Emericellamide C (14) against E. coli and C. albicans. Conclusion These results will expand the knowledge of endophytes and provide us with new approaches to face the global antibiotic resistance problem and the future production of undiscovered compounds different from the antibiotics classes. |
| first_indexed | 2024-03-10T22:13:04Z |
| format | Article |
| id | doaj.art-23aad25fe942445ea93e4ed436c4f4ae |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-10T22:13:04Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-23aad25fe942445ea93e4ed436c4f4ae2023-11-19T12:32:45ZengBMCBMC Microbiology1471-21802023-11-0123111910.1186/s12866-023-03092-5Evaluation of the anti-infective potential of the seed endophytic fungi of Corchorus olitorius through metabolomics and molecular docking approachArwa Mortada Ahmed0Ayman M. Ibrahim1Ramadan Yahia2Nourhan Hisham Shady3Basma Khalaf Mahmoud4Usama Ramadan Abdelmohsen5Mostafa A. Fouad6Department of Pharmacognosy, Faculty of Pharmacy, Daraya UniversityDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Daraya UniversityDepartment of Microbiology and Immunology, Faculty of Pharmacy, Daraya UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Daraya UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Minia UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Daraya UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Minia UniversityAbstract Background Endophytic fungi are very rich sources of natural antibacterial and antifungal compounds. The main aim of this study is to isolate the fungal endophytes from the medicinal plant Corchorus olitorius seeds (F. Malvaceae), followed by antimicrobial screening against various bacterial and fungal strains. Results Seven endophytic fungal strains belonging to different three genera were isolated, including Penicillium, Fusarium, and Aspergillus. The seven isolated endophytic strains revealed selective noticeable activity against Escherichia coli (ATCC25922) with varied IC50s ranging from 1.19 to 10 µg /mL, in which Aspergillus sp. (Ar 6) exhibited the strongest potency against E. coli (ATCC 25,922) and candida albicans (ATCC 10,231) with IC50s 1.19 and 15 µg /mL, respectively. Therefore, the chemical profiling of Aspergillus sp. (Ar 6) crude extract was performed using LC-HR-ESI-MS and led to the dereplication of sixteen compounds of various classes (1–16). In-silico analysis of the dereplicated metabolites led to highlighting the compounds responsible for the antimicrobial activity of Aspergillus sp. extract. Moreover, molecular docking showed the potential targets of the metabolites; Astellatol (5), Aspergillipeptide A (10), and Emericellamide C (14) against E. coli and C. albicans. Conclusion These results will expand the knowledge of endophytes and provide us with new approaches to face the global antibiotic resistance problem and the future production of undiscovered compounds different from the antibiotics classes.https://doi.org/10.1186/s12866-023-03092-5Antimicrobial activityCorchorus olitorius seedsEndophytic fungiDereplicationMolecular docking. |
| spellingShingle | Arwa Mortada Ahmed Ayman M. Ibrahim Ramadan Yahia Nourhan Hisham Shady Basma Khalaf Mahmoud Usama Ramadan Abdelmohsen Mostafa A. Fouad Evaluation of the anti-infective potential of the seed endophytic fungi of Corchorus olitorius through metabolomics and molecular docking approach BMC Microbiology Antimicrobial activity Corchorus olitorius seeds Endophytic fungi Dereplication Molecular docking. |
| title | Evaluation of the anti-infective potential of the seed endophytic fungi of Corchorus olitorius through metabolomics and molecular docking approach |
| title_full | Evaluation of the anti-infective potential of the seed endophytic fungi of Corchorus olitorius through metabolomics and molecular docking approach |
| title_fullStr | Evaluation of the anti-infective potential of the seed endophytic fungi of Corchorus olitorius through metabolomics and molecular docking approach |
| title_full_unstemmed | Evaluation of the anti-infective potential of the seed endophytic fungi of Corchorus olitorius through metabolomics and molecular docking approach |
| title_short | Evaluation of the anti-infective potential of the seed endophytic fungi of Corchorus olitorius through metabolomics and molecular docking approach |
| title_sort | evaluation of the anti infective potential of the seed endophytic fungi of corchorus olitorius through metabolomics and molecular docking approach |
| topic | Antimicrobial activity Corchorus olitorius seeds Endophytic fungi Dereplication Molecular docking. |
| url | https://doi.org/10.1186/s12866-023-03092-5 |
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