Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC
Background Gastric cancer (GC) is a global leading source of cancer-associated deaths. Circular RNAs (circRNAs) are a new type of non-coding RNA and promising biomarkers for diagnosis of multiple diseases such as cancer.Methods Circ-PRMT5 expression was validated in 90 GC patient tissues and 6 diffe...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1671857 |
| _version_ | 1817981442032926720 |
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| author | Wenbo Du Ding Li Xiaomin Guo Ping Li Xin Li Shuping Tong Junhui Tong Lihua Kuang Daoming Liang |
| author_facet | Wenbo Du Ding Li Xiaomin Guo Ping Li Xin Li Shuping Tong Junhui Tong Lihua Kuang Daoming Liang |
| author_sort | Wenbo Du |
| collection | DOAJ |
| description | Background Gastric cancer (GC) is a global leading source of cancer-associated deaths. Circular RNAs (circRNAs) are a new type of non-coding RNA and promising biomarkers for diagnosis of multiple diseases such as cancer.Methods Circ-PRMT5 expression was validated in 90 GC patient tissues and 6 different GC cells by qRT-PCR. Sublocalization of circ-PRMT5 in GC cells was determined in isolated nuclear and cytoplasmic RNAs. CircInteractome and miRanda were used to predict binding sites between circ-PRMT5 with micRNAs, and micRNAs with target mRNA. The correlation between genes was determined by the Pearson correlation analysis. The molecular mechanism was demonstrated by RNA in vivo precipitation, point mutation, luciferase activity and rescue experiments.Results Circ-PRMT5 expression was significantly higher in GC than in adjacent normal tissues, and GC patients with circ-PRMT5 high expression had shorter survival times. Functionally, circ-PRMT5 silence inhibited GC cell growth and invasion. Mechanism analysis showed that circ-PRMT5 sponged miR-145/miR-1304 to upregulate MYC expression and GC development.Conclusion Our findings demonstrated that circ-PRMT5 function as an oncogene in GC patients by targeting miR-145/miR-1304/MYC axis. High circ-PRMT5 expression may provide a poor prognostic indicator of survival in GC patients and targeting circ-PRMT5/miR-145/miR-1304/MYC axis may be a novel therapeutic strategy for GC. |
| first_indexed | 2024-04-13T23:05:55Z |
| format | Article |
| id | doaj.art-23ab1a66b6e14564bf9ddd61c5f81f3f |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T23:05:55Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-23ab1a66b6e14564bf9ddd61c5f81f3f2022-12-22T02:25:41ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014714120413010.1080/21691401.2019.1671857Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYCWenbo Du0Ding Li1Xiaomin Guo2Ping Li3Xin Li4Shuping Tong5Junhui Tong6Lihua Kuang7Daoming Liang8Department of Gastroenterology, The Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia, ChinaDepartment of General Surgery, Nantong Tumor Hospital, Nantong, Jiangsu, ChinaSchool of Mongol Medicine, Chuxiong Medical College, Chuxiong, Yunnan, ChinaAcademic Office, Hainan Medical University, Haikou, Hainan, ChinaSchool of Mongol Medicine, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia, ChinaDepartment of Respiratory and Critical Care Medicine, The Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia, ChinaDepartment of Pharmacology, School of Pharmacy, China Medical University, Shenyang, Liaoning, ChinaDepartment of Internal Medicine, Shenzhen Pingle Orthopaedic Hospital, Pingshan New District, Shenzhen, ChinaDepartment of Gastrointestinal Surgery, Kunming Medical University Second Hospital, Kunming, Yunnan, ChinaBackground Gastric cancer (GC) is a global leading source of cancer-associated deaths. Circular RNAs (circRNAs) are a new type of non-coding RNA and promising biomarkers for diagnosis of multiple diseases such as cancer.Methods Circ-PRMT5 expression was validated in 90 GC patient tissues and 6 different GC cells by qRT-PCR. Sublocalization of circ-PRMT5 in GC cells was determined in isolated nuclear and cytoplasmic RNAs. CircInteractome and miRanda were used to predict binding sites between circ-PRMT5 with micRNAs, and micRNAs with target mRNA. The correlation between genes was determined by the Pearson correlation analysis. The molecular mechanism was demonstrated by RNA in vivo precipitation, point mutation, luciferase activity and rescue experiments.Results Circ-PRMT5 expression was significantly higher in GC than in adjacent normal tissues, and GC patients with circ-PRMT5 high expression had shorter survival times. Functionally, circ-PRMT5 silence inhibited GC cell growth and invasion. Mechanism analysis showed that circ-PRMT5 sponged miR-145/miR-1304 to upregulate MYC expression and GC development.Conclusion Our findings demonstrated that circ-PRMT5 function as an oncogene in GC patients by targeting miR-145/miR-1304/MYC axis. High circ-PRMT5 expression may provide a poor prognostic indicator of survival in GC patients and targeting circ-PRMT5/miR-145/miR-1304/MYC axis may be a novel therapeutic strategy for GC.https://www.tandfonline.com/doi/10.1080/21691401.2019.1671857Gastric cancercirc-PRMT5miR-145miR-1304MYCinvasion |
| spellingShingle | Wenbo Du Ding Li Xiaomin Guo Ping Li Xin Li Shuping Tong Junhui Tong Lihua Kuang Daoming Liang Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC Artificial Cells, Nanomedicine, and Biotechnology Gastric cancer circ-PRMT5 miR-145 miR-1304 MYC invasion |
| title | Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC |
| title_full | Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC |
| title_fullStr | Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC |
| title_full_unstemmed | Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC |
| title_short | Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC |
| title_sort | circ prmt5 promotes gastric cancer progression by sponging mir 145 and mir 1304 to upregulate myc |
| topic | Gastric cancer circ-PRMT5 miR-145 miR-1304 MYC invasion |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1671857 |
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