Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran)
Background: Asafoetida (Ferula assa-foetida L.) is an endangered endemic plant which is widely used in food, cosmetics and pharmaceutical industries. It is necessary to enhance the quantity and quality of its secondary metabolites in order to meet requirements of the industries. Objective: This stud...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2021-01-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-3010-en.html |
| _version_ | 1819063678717132800 |
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| author | Amir Saadatfar Samira Hossein Jafari Iraj Tavassolian |
| author_facet | Amir Saadatfar Samira Hossein Jafari Iraj Tavassolian |
| author_sort | Amir Saadatfar |
| collection | DOAJ |
| description | Background: Asafoetida (Ferula assa-foetida L.) is an endangered endemic plant which is widely used in food, cosmetics and pharmaceutical industries. It is necessary to enhance the quantity and quality of its secondary metabolites in order to meet requirements of the industries. Objective: This study investigated the impact of 24-epibrassinolide (EBL) on biochemical traits, essential oil yield and chemical compositions of Ferula assa-foetida in its natural habitat. Methods: We foliar sprayed the EBL at the concentrations of 0, 0.1, 0.5 and 1 µM with three times during a week at fully matured leaves phase. One month after treatments application, leaf sampling and gum gathering of the plants was done and the mentioned parameters were measured. Results: ANOVA results indicated that 1 µM EBL showed the best results for chlorophyll a, b, total chlorophyll, carotenoids, reduced sugar and protein contents (17.77, 13.08, 31.57, 7.50, 40.45 and 0.34 mg/g FW). The main components of the essential oil are α-pinene (8.82 %), β-pinene (11.57 %) and myrcene (1.12 %) that showed a significant increase at 1 µM of EBL. Although, EBL at 0.5 µM was proved to be the best treatment for leaf total phenol content (79.37 mg/g FW) and sulfurous compounds such as: (E)-1-propenyl sec-butyl disulfide (51.48 %), bis (1-methyl propyl) disulfide (0.9 %) and n-propyl sec-butyl disulfide (0.41 %). The highest amount of flavonoids (146.18; 162.56 mg/g FW) and essential oil yield (7.91 %; 8.16 %) were obtained at 0.5 and 1 µM EBL concentrations, respectively compared to the control (6 %). Conclusion: Our results indicated the promising and positive effects of EBL, as an environmental friendly strategy, to improve oleo-gum quantity and quality. |
| first_indexed | 2024-12-21T15:18:30Z |
| format | Article |
| id | doaj.art-23ace6b23fb64891a6e1e78368237f60 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-21T15:18:30Z |
| publishDate | 2021-01-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-23ace6b23fb64891a6e1e78368237f602022-12-21T18:59:05ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582021-01-01207793107Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran)Amir Saadatfar0Samira Hossein Jafari1Iraj Tavassolian2 Department of Medicinal Plants, Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, Kerman, Iran Post-doctoral researcher, Faculty of Natural Resources and Desert Study, Yazd University, Yazd, Iran Department of Rangeland and Watershed Management, Higher Education Complex of Shirvan, Shirvan, Iran Background: Asafoetida (Ferula assa-foetida L.) is an endangered endemic plant which is widely used in food, cosmetics and pharmaceutical industries. It is necessary to enhance the quantity and quality of its secondary metabolites in order to meet requirements of the industries. Objective: This study investigated the impact of 24-epibrassinolide (EBL) on biochemical traits, essential oil yield and chemical compositions of Ferula assa-foetida in its natural habitat. Methods: We foliar sprayed the EBL at the concentrations of 0, 0.1, 0.5 and 1 µM with three times during a week at fully matured leaves phase. One month after treatments application, leaf sampling and gum gathering of the plants was done and the mentioned parameters were measured. Results: ANOVA results indicated that 1 µM EBL showed the best results for chlorophyll a, b, total chlorophyll, carotenoids, reduced sugar and protein contents (17.77, 13.08, 31.57, 7.50, 40.45 and 0.34 mg/g FW). The main components of the essential oil are α-pinene (8.82 %), β-pinene (11.57 %) and myrcene (1.12 %) that showed a significant increase at 1 µM of EBL. Although, EBL at 0.5 µM was proved to be the best treatment for leaf total phenol content (79.37 mg/g FW) and sulfurous compounds such as: (E)-1-propenyl sec-butyl disulfide (51.48 %), bis (1-methyl propyl) disulfide (0.9 %) and n-propyl sec-butyl disulfide (0.41 %). The highest amount of flavonoids (146.18; 162.56 mg/g FW) and essential oil yield (7.91 %; 8.16 %) were obtained at 0.5 and 1 µM EBL concentrations, respectively compared to the control (6 %). Conclusion: Our results indicated the promising and positive effects of EBL, as an environmental friendly strategy, to improve oleo-gum quantity and quality.http://jmp.ir/article-1-3010-en.htmlsecondary metabolitesbiochemical traitschemical compositions<i>ferula assa-foetida</i> l.natural habitat |
| spellingShingle | Amir Saadatfar Samira Hossein Jafari Iraj Tavassolian Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran) Journal of Medicinal Plants secondary metabolites biochemical traits chemical compositions <i>ferula assa-foetida</i> l. natural habitat |
| title | Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran) |
| title_full | Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran) |
| title_fullStr | Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran) |
| title_full_unstemmed | Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran) |
| title_short | Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran) |
| title_sort | improving biochemical traits oleo gum yield and compositions of asafoetida ferula assa foetida l essential oil using 24 epibrassinolide in kerman natural habitats iran |
| topic | secondary metabolites biochemical traits chemical compositions <i>ferula assa-foetida</i> l. natural habitat |
| url | http://jmp.ir/article-1-3010-en.html |
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