Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus
Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruli...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2013-09-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174806/ |
| _version_ | 1828321733652774912 |
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| author | Joo Hyun Lee Jae Young Choi Xue Ying Tao Jae Su Kim Woojin Kim Yeon Ho Je |
| author_facet | Joo Hyun Lee Jae Young Choi Xue Ying Tao Jae Su Kim Woojin Kim Yeon Ho Je |
| author_sort | Joo Hyun Lee |
| collection | DOAJ |
| description | Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of isotigs from the mRNA of RVLS and NVLS, respectively. The gene ontology (GO) analysis demonstrated that both libraries have similar GO structures, however, the gene expression pattern analysis revealed that 17.8% and 16.8% of isotigs were up- and down-regulated significantly in the RVLS, respectively. These RSV-dependently regulated genes possibly have important roles in the physiology of SBPH, transmission of RSV, and RSV and SBPH interaction. |
| first_indexed | 2024-04-13T18:31:13Z |
| format | Article |
| id | doaj.art-23ae4115cba148098b412969b003a441 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-04-13T18:31:13Z |
| publishDate | 2013-09-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-23ae4115cba148098b412969b003a4412022-12-22T02:35:05ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542013-09-0129333033710.5423/PPJ.NT.01.2013.0001PPJ.NT.01.2013.0001Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virusJoo Hyun Lee0Jae Young Choi1Xue Ying Tao2Jae Su Kim3Woojin Kim4Yeon Ho Je5Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, KoreaResearch Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, KoreaDepartment of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, KoreaDepartment of Agricultural Biology, College of Agricultural Life Sciences, Chonbuk National University, Jeonju 561-756, KoreaResearch Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, KoreaDepartment of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, KoreaRice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of isotigs from the mRNA of RVLS and NVLS, respectively. The gene ontology (GO) analysis demonstrated that both libraries have similar GO structures, however, the gene expression pattern analysis revealed that 17.8% and 16.8% of isotigs were up- and down-regulated significantly in the RVLS, respectively. These RSV-dependently regulated genes possibly have important roles in the physiology of SBPH, transmission of RSV, and RSV and SBPH interaction.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174806/Laodelphax striatellusRice stripe virussmall brown planthoppertenuivirustranscriptome |
| spellingShingle | Joo Hyun Lee Jae Young Choi Xue Ying Tao Jae Su Kim Woojin Kim Yeon Ho Je Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus The Plant Pathology Journal Laodelphax striatellus Rice stripe virus small brown planthopper tenuivirus transcriptome |
| title | Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus |
| title_full | Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus |
| title_fullStr | Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus |
| title_full_unstemmed | Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus |
| title_short | Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus |
| title_sort | transcriptome analysis of the small brown planthopper laodelphax striatellus carrying rice stripe virus |
| topic | Laodelphax striatellus Rice stripe virus small brown planthopper tenuivirus transcriptome |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174806/ |
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