Broadening the Substrate Specificity of Cellobiose Phosphorylase from <i>Clostridium thermocellum</i> for Improved Transformation of Cellodextrin to Starch

Broadening the Substrate Specificity of Cellobiose Phosphorylase from <i>Clostridium thermocellum</i> for Improved Transformation of Cellodextrin to Starch

Cellobiose phosphorylase (CBP) catalyzes the reversible phosphorolysis of cellobiose into α-glucose 1-phosphate and glucose. A CBP with a broadened substrate specificity would be more desirable when utilized to convert cellulose into amylose (<i>PNAS</i>, 110: 7182–7187, 2013) and to con...

Full description

Bibliographic Details
Main Authors:	Yuanyuan Zhang, Yapeng Li, Hui Lin, Guotao Mao, Xiang Long, Xinyu Liu, Hongge Chen
Format:	Article
Language:	English
Published:	MDPI AG 2023-09-01
Series:	International Journal of Molecular Sciences
Subjects:	cellobiose phosphorylase cellodextrin phosphorylase substrate specificity cellodextrin amylose
Online Access:	https://www.mdpi.com/1422-0067/24/19/14452

Similar Items

In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel in Ruminiclostridium cellulolyticum: implication for cellulose catabolism
by: Nian Liu, et al.
Published: (2019-09-01)

Insights to improve the activity of glycosyl phosphorylases from Ruminococcus albus 8 with cello-oligosaccharides
by: Alem Storani, et al.
Published: (2023-04-01)

Identification and Characterization of a Cellodextrin Transporter in Aspergillus niger
by: Hui Lin, et al.
Published: (2020-02-01)

A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis
by: Aurélie Fosses, et al.
Published: (2017-10-01)

PHB production from cellobiose with Saccharomyces cerevisiae
by: Anna Ylinen, et al.
Published: (2022-06-01)

Installing xylose assimilation and cellodextrin phosphorolysis pathways in obese Yarrowia lipolytica facilitates cost-effective lipid production from lignocellulosic hydrolysates
by: Yiran Zhang, et al.
Published: (2023-11-01)

MALDI-TOF MS Analysis of Cellodextrins and Xylo-oligosaccharides Produced by Hindgut Homogenates of Reticulitermes santonensis
by: Catherine Brasseur, et al.
Published: (2014-04-01)

Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides
by: Mario Klimacek, et al.
Published: (2021-06-01)

Molecular Functions and Pathways of Plastidial Starch Phosphorylase (PHO1) in Starch Metabolism: Current and Future Perspectives
by: Noman Shoaib, et al.
Published: (2021-09-01)

Comparative Study of Starch Phosphorylase Genes and Encoded Proteins in Various Monocots and Dicots with Emphasis on Maize
by: Guowu Yu, et al.
Published: (2022-04-01)

Engineering of a new Escherichia coli strain efficiently metabolizing cellobiose with promising perspectives for plant biomass-based application design
by: Romain Borne, et al.
Published: (2021-06-01)

Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem
by: Dongdong Meng, et al.
Published: (2019-11-01)

Strained Conformations of Nucleosides in Active Sites of Nucleoside Phosphorylases
by: Irina A. Il’icheva, et al.
Published: (2020-04-01)

Preparation and Applications of Amylose Supramolecules by Means of Phosphorylase-Catalyzed Enzymatic Polymerization
by: Jun-ichi Kadokawa
Published: (2012-01-01)

Brain-Type Glycogen Phosphorylase (PYGB) in the Pathologies of Diseases: A Systematic Review
by: Caiting Yang, et al.
Published: (2024-02-01)

Vine-Twining Inclusion Behavior of Amylose towards Hydrophobic Polyester, Poly(β-propiolactone), in Glucan Phosphorylase-Catalyzed Enzymatic Polymerization
by: Masa-aki Iwamoto, et al.
Published: (2023-01-01)

Oligomeric Symmetry of Purine Nucleoside Phosphorylases
by: Boris Gomaz, et al.
Published: (2024-01-01)

The role of glycogen phosphorylase in glycogen biogenesis in skeletal muscle after exercise
by: Abram Katz
Published: (2023-03-01)

Thymidine phosphorylase and its possible correlation with the lung cancer phenotype features
by: E. M. Bakurova, et al.
Published: (2015-04-01)

Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar using RNA-Seq
by: Hui eWei, et al.
Published: (2014-04-01)

Lipid Peroxidation and Thymidine Phosphorylase expression in Prostate Carcinoma
by: Tandon R., et al.
Published: (2012-08-01)

Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering
by: Jorick Franceus, et al.
Published: (2020-04-01)

X-ray crystallographic studies of glycogen phosphorylase b
by: Wild, D, et al.
Published: (1981)

Pho1a (plastid starch phosphorylase) is duplicated and essential for normal starch granule phenotype in tubers of Solanum tuberosum L
by: Shrikant Sharma, et al.
Published: (2023-08-01)

Synthesis, in vitro thymidine phosphorylase inhibitory activity and molecular docking study of novel pyridine-derived bis-oxadiazole bearing bis-schiff base derivatives
by: Rafaqat Hussain, et al.
Published: (2023-06-01)

Biocatalytic Process for Production of α-Glucosylglycerol Using Sucrose Phosphorylase
by: Christiane Luley-Goedl, et al.
Published: (2010-01-01)

Optimization and Validation of an In Vitro Standardized Glycogen Phosphorylase Activity Assay
by: Sónia Rocha, et al.
Published: (2021-07-01)

Fabrication of Nanostructured Supramolecules through Helical Inclusion of Amylose toward Hydrophobic Polyester Guests, Biomimetically through Vine-Twining Polymerization Process
by: Jun-ichi Kadokawa
Published: (2023-11-01)

QM/MM Study of a Nucleophilic Substitution Reaction Catalyzed by Uridine Phosphorylase from <i>Vibrio cholerae</i>
by: Alexander A. Lashkov, et al.
Published: (2023-05-01)

Overexpression of the starch phosphorylase-like gene (PHO3) in Lotus japonicus has a profound effect on the growth of plants and reduction of transitory starch accumulation
by: Shanshan Qin, et al.
Published: (2016-08-01)

Synthesis of Thymidine Phosphorylase Inhibitor Based on Quinoxaline Derivatives and Their Molecular Docking Study
by: Noor Barak Almandil, et al.
Published: (2019-03-01)

Efficient Synthesis of Purine Nucleoside Analogs by a New Trimeric Purine Nucleoside Phosphorylase from <i>Aneurinibacillus migulanus</i> AM007
by: Gaofei Liu, et al.
Published: (2019-12-01)

Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases
by: Ao Li, et al.
Published: (2022-03-01)

Comparative genomics reveals cellobiose hydrolysis mechanism of Ruminiclostridium thermocellum M3, a cellulosic saccharification bacterium
by: Sheng Tao, et al.
Published: (2023-01-01)

Determining the specific activity of thymidine phosphorylase in leukocytes of patients with MNGIE and the plasma thymidine level by RP-HPLC
by: Rezaei Sh, et al.
Published: (2011-06-01)

Partial Purine Nucleoside Phosphorylase Deficiency Helps Determine Minimal Activity Required for Immune and Neurological Development
by: Eyal Grunebaum, et al.
Published: (2020-06-01)

Research development of the relationship between thymidine phosphorylase expression and colorectal carcinoma
by: Dian-Jun Ye, et al.
Published: (2013-03-01)

Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli
by: Thomas Carzaniga, et al.
Published: (2017-04-01)

Pseudomonas aeruginosa Polynucleotide Phosphorylase Contributes to Ciprofloxacin Resistance by Regulating PrtR
by: Zheng Fan, et al.
Published: (2019-07-01)

Tricyclic Nucleobase Analogs and Their Ribosides as Substrates and Inhibitors of Purine-Nucleoside Phosphorylases III. Aminopurine Derivatives
by: Alicja Stachelska-Wierzchowska, et al.
Published: (2020-02-01)