Molecular Characterisation of Antibiotic Resistance in Staphylococcus haemolyticus Isolates from Chennai, South India
Staphylococcus haemolyticus is a highly resistant opportunistic pathogen having close genomic relatedness with other virulent species of staphylococci. However, compared to Staphylococcus aureus and Staphylococcus epidermidis, little is known about the resistance genes of S. haemolyticus. The purpos...
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| Format: | Article |
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Journal of Pure and Applied Microbiology
2022-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/molecular-characterisation-of-antibiotic-resistance-in-staphylococcus-haemolyticus-isolates-from-chennai-south-india/ |
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| author | Anbarasi Kalaiselvan Padma Krishnan Esther Mary Selvam |
| author_facet | Anbarasi Kalaiselvan Padma Krishnan Esther Mary Selvam |
| author_sort | Anbarasi Kalaiselvan |
| collection | DOAJ |
| description | Staphylococcus haemolyticus is a highly resistant opportunistic pathogen having close genomic relatedness with other virulent species of staphylococci. However, compared to Staphylococcus aureus and Staphylococcus epidermidis, little is known about the resistance genes of S. haemolyticus. The purpose of this study was to characterise antibiotic resistance genes in S. haemolyticus isolates. Standard microbiological techniques were used to identify and confirm 104 S. haemolyticus isolates included in the study. Antibiotic susceptibility testing and D-test were performed, followed by PCR amplification of various resistance determinants (mecA, ermA, ermC, msrA, aac(6′)-Ie-aph(2″), ant(4′)-Ia,aph(3′)-IIIa, tetK, tetM, dfrA, fusB, fusC, fusD and mupA). Methicillin resistance was observed in 93.3% of study isolates. The maximum number of isolates showed resistance to erythromycin (n=79, 76%), followed by ciprofloxacin (n=66, 63.5%) and cotrimoxazole (n=58, 55.8%). In the D-test, 8 isolates showed inducible (iMLSB) and 11 showed constitutive (cMLSB) resistance. Among the resistance determinants, mecA gene (93.3%) was the most prevalent, followed by dfrA (50.5%). Furthermore, aac(6’)-Ie-aph(2’’) and aph(3’)-IIIa combination was observed in 26.9% of isolates, and aac(6’)-Ie-aph(2’’) alone was present in 3.8% of isolates. Among the study isolates, 17.3% exhibited tetK gene, whereas only 1% exhibited tetM; a combination of tetK and tetM was observed in one isolate. The fusB and fusC were present in 11.5% of isolates, and 12.5% of the isolates were positive for mupA. In conclusion, the present study underlines the concern of increasing antibiotic resistance among S. haemolyticus isolates. Avoiding misuse/overuse of antibiotics along with continuous surveillance programs can reduce the spread of antibiotic resistance. |
| first_indexed | 2024-04-11T16:42:21Z |
| format | Article |
| id | doaj.art-23b1d1af1cf14d78aae023f3555b522a |
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| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-11T16:42:21Z |
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| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-23b1d1af1cf14d78aae023f3555b522a2022-12-22T04:13:39ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2022-09-0116320552065https://doi.org/10.22207/JPAM.16.3.60Molecular Characterisation of Antibiotic Resistance in Staphylococcus haemolyticus Isolates from Chennai, South IndiaAnbarasi Kalaiselvanhttps://orcid.org/0000-0002-6533-1527Padma Krishnanhttps://orcid.org/0000-0003-0158-8953Esther Mary SelvamStaphylococcus haemolyticus is a highly resistant opportunistic pathogen having close genomic relatedness with other virulent species of staphylococci. However, compared to Staphylococcus aureus and Staphylococcus epidermidis, little is known about the resistance genes of S. haemolyticus. The purpose of this study was to characterise antibiotic resistance genes in S. haemolyticus isolates. Standard microbiological techniques were used to identify and confirm 104 S. haemolyticus isolates included in the study. Antibiotic susceptibility testing and D-test were performed, followed by PCR amplification of various resistance determinants (mecA, ermA, ermC, msrA, aac(6′)-Ie-aph(2″), ant(4′)-Ia,aph(3′)-IIIa, tetK, tetM, dfrA, fusB, fusC, fusD and mupA). Methicillin resistance was observed in 93.3% of study isolates. The maximum number of isolates showed resistance to erythromycin (n=79, 76%), followed by ciprofloxacin (n=66, 63.5%) and cotrimoxazole (n=58, 55.8%). In the D-test, 8 isolates showed inducible (iMLSB) and 11 showed constitutive (cMLSB) resistance. Among the resistance determinants, mecA gene (93.3%) was the most prevalent, followed by dfrA (50.5%). Furthermore, aac(6’)-Ie-aph(2’’) and aph(3’)-IIIa combination was observed in 26.9% of isolates, and aac(6’)-Ie-aph(2’’) alone was present in 3.8% of isolates. Among the study isolates, 17.3% exhibited tetK gene, whereas only 1% exhibited tetM; a combination of tetK and tetM was observed in one isolate. The fusB and fusC were present in 11.5% of isolates, and 12.5% of the isolates were positive for mupA. In conclusion, the present study underlines the concern of increasing antibiotic resistance among S. haemolyticus isolates. Avoiding misuse/overuse of antibiotics along with continuous surveillance programs can reduce the spread of antibiotic resistance.https://microbiologyjournal.org/molecular-characterisation-of-antibiotic-resistance-in-staphylococcus-haemolyticus-isolates-from-chennai-south-india/s. haemolyticus resistancemultidrug-resistant s. haemolyticusantibiotic drug resistance |
| spellingShingle | Anbarasi Kalaiselvan Padma Krishnan Esther Mary Selvam Molecular Characterisation of Antibiotic Resistance in Staphylococcus haemolyticus Isolates from Chennai, South India Journal of Pure and Applied Microbiology s. haemolyticus resistance multidrug-resistant s. haemolyticus antibiotic drug resistance |
| title | Molecular Characterisation of Antibiotic Resistance in Staphylococcus haemolyticus Isolates from Chennai, South India |
| title_full | Molecular Characterisation of Antibiotic Resistance in Staphylococcus haemolyticus Isolates from Chennai, South India |
| title_fullStr | Molecular Characterisation of Antibiotic Resistance in Staphylococcus haemolyticus Isolates from Chennai, South India |
| title_full_unstemmed | Molecular Characterisation of Antibiotic Resistance in Staphylococcus haemolyticus Isolates from Chennai, South India |
| title_short | Molecular Characterisation of Antibiotic Resistance in Staphylococcus haemolyticus Isolates from Chennai, South India |
| title_sort | molecular characterisation of antibiotic resistance in staphylococcus haemolyticus isolates from chennai south india |
| topic | s. haemolyticus resistance multidrug-resistant s. haemolyticus antibiotic drug resistance |
| url | https://microbiologyjournal.org/molecular-characterisation-of-antibiotic-resistance-in-staphylococcus-haemolyticus-isolates-from-chennai-south-india/ |
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