CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK

We studied the viability and dynamic of cell distribution during long-term cultivation of broblasts 3T3 in spider silk spidroin 1-based scaffold. Laser scanning confocal microscopy is shown to have advantages for visualization of cells situated on the external and internal surfaces of scaffold. Fibr...

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Main Authors: O. L. Pustovalova, I. I. Agapov, M. M. Moisenovich, P. S. Eremin, A. A. Kazulina, A. Y. Arhipova, A. A. Ramonova, V. G. Bogush, V. I. Sevastianov, M. P. Kirpichnikov
Format: Article
Language:Russian
Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 2009-05-01
Series:Vestnik Transplantologii i Iskusstvennyh Organov
Subjects:
Online Access:https://journal.transpl.ru/vtio/article/view/233
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author O. L. Pustovalova
I. I. Agapov
M. M. Moisenovich
P. S. Eremin
A. A. Kazulina
A. Y. Arhipova
A. A. Ramonova
V. G. Bogush
V. I. Sevastianov
M. P. Kirpichnikov
author_facet O. L. Pustovalova
I. I. Agapov
M. M. Moisenovich
P. S. Eremin
A. A. Kazulina
A. Y. Arhipova
A. A. Ramonova
V. G. Bogush
V. I. Sevastianov
M. P. Kirpichnikov
author_sort O. L. Pustovalova
collection DOAJ
description We studied the viability and dynamic of cell distribution during long-term cultivation of broblasts 3T3 in spider silk spidroin 1-based scaffold. Laser scanning confocal microscopy is shown to have advantages for visualization of cells situated on the external and internal surfaces of scaffold. Fibroblasts maintain high proliferative ability and viability during long term cultivation. Spidroin 1-based scaffold are the perspective materials for bioengineering.
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last_indexed 2024-04-10T00:53:47Z
publishDate 2009-05-01
publisher Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
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series Vestnik Transplantologii i Iskusstvennyh Organov
spelling doaj.art-23b471ea0aa94b3f905c7a9fcd8fecb62023-03-13T10:37:19ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovVestnik Transplantologii i Iskusstvennyh Organov1995-11912009-05-01112545910.15825/1995-1191-2009-2-54-59176CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILKO. L. Pustovalova0I. I. Agapov1M. M. Moisenovich2P. S. Eremin3A. A. Kazulina4A. Y. Arhipova5A. A. Ramonova6V. G. Bogush7V. I. Sevastianov8M. P. Kirpichnikov9Moscow State University, biological facultyAcademician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, MoscowMoscow State University, biological facultyMoscow State University, biological facultyMoscow State University, biological facultyMoscow State University, biological facultyMoscow State University, biological facultyState Scientific Center «GNIIgenetika»Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, MoscowMoscow State University, biological facultyWe studied the viability and dynamic of cell distribution during long-term cultivation of broblasts 3T3 in spider silk spidroin 1-based scaffold. Laser scanning confocal microscopy is shown to have advantages for visualization of cells situated on the external and internal surfaces of scaffold. Fibroblasts maintain high proliferative ability and viability during long term cultivation. Spidroin 1-based scaffold are the perspective materials for bioengineering.https://journal.transpl.ru/vtio/article/view/233tissue engineeringrecombinant spider silk
spellingShingle O. L. Pustovalova
I. I. Agapov
M. M. Moisenovich
P. S. Eremin
A. A. Kazulina
A. Y. Arhipova
A. A. Ramonova
V. G. Bogush
V. I. Sevastianov
M. P. Kirpichnikov
CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
Vestnik Transplantologii i Iskusstvennyh Organov
tissue engineering
recombinant spider silk
title CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_full CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_fullStr CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_full_unstemmed CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_short CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_sort confocal microscopy study of biological peculiarities of scaffold made from recombinant spider silk
topic tissue engineering
recombinant spider silk
url https://journal.transpl.ru/vtio/article/view/233
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