A protocol to assess cellular bioenergetics in flavivirus-infected cells

A protocol to assess cellular bioenergetics in flavivirus-infected cells

Summary: Aberrant cellular bioenergetics has detrimental consequences in host cells. For instance, pathogenic Zika virus strains can suppress mitochondria respiration and glycolytic functions, disrupting cellular bioenergetics that leads to apoptosis. Herein, we describe methods for flavivirus propa...

Full description

Bibliographic Details
Main Authors: John Z.H. Low, Clement Yau, Summer L.X. Zhang, Hwee Cheng Tan, Eng Eong Ooi, Kuan Rong Chan
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell-based Assays
Metabolism
Microbiology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001770
Description
Summary:Summary: Aberrant cellular bioenergetics has detrimental consequences in host cells. For instance, pathogenic Zika virus strains can suppress mitochondria respiration and glycolytic functions, disrupting cellular bioenergetics that leads to apoptosis. Herein, we describe methods for flavivirus propagation, titering and infection, cell preparation, and procedures for mitochondrial and glycolytic stress tests. The protocol enables assessment of cellular respiration and glycolytic flux in flavivirus-infected cells.For complete details on the use and execution of this protocol, please refer to Yau et al. (2021).
ISSN:2666-1667

Similar Items