A protocol to assess cellular bioenergetics in flavivirus-infected cells
Summary: Aberrant cellular bioenergetics has detrimental consequences in host cells. For instance, pathogenic Zika virus strains can suppress mitochondria respiration and glycolytic functions, disrupting cellular bioenergetics that leads to apoptosis. Herein, we describe methods for flavivirus propa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722001770 |
| _version_ | 1818060173452771328 |
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| author | John Z.H. Low Clement Yau Summer L.X. Zhang Hwee Cheng Tan Eng Eong Ooi Kuan Rong Chan |
| author_facet | John Z.H. Low Clement Yau Summer L.X. Zhang Hwee Cheng Tan Eng Eong Ooi Kuan Rong Chan |
| author_sort | John Z.H. Low |
| collection | DOAJ |
| description | Summary: Aberrant cellular bioenergetics has detrimental consequences in host cells. For instance, pathogenic Zika virus strains can suppress mitochondria respiration and glycolytic functions, disrupting cellular bioenergetics that leads to apoptosis. Herein, we describe methods for flavivirus propagation, titering and infection, cell preparation, and procedures for mitochondrial and glycolytic stress tests. The protocol enables assessment of cellular respiration and glycolytic flux in flavivirus-infected cells.For complete details on the use and execution of this protocol, please refer to Yau et al. (2021). |
| first_indexed | 2024-12-10T13:28:13Z |
| format | Article |
| id | doaj.art-23b60498dae54c3da1ab9f474195b662 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-10T13:28:13Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-23b60498dae54c3da1ab9f474195b6622022-12-22T01:47:05ZengElsevierSTAR Protocols2666-16672022-06-0132101297A protocol to assess cellular bioenergetics in flavivirus-infected cellsJohn Z.H. Low0Clement Yau1Summer L.X. Zhang2Hwee Cheng Tan3Eng Eong Ooi4Kuan Rong Chan5Programme in Emerging Infectious Diseases, Duke-NUS Medical School, 8 College Road, Singapore 169857, SingaporeProgramme in Emerging Infectious Diseases, Duke-NUS Medical School, 8 College Road, Singapore 169857, SingaporeProgramme in Emerging Infectious Diseases, Duke-NUS Medical School, 8 College Road, Singapore 169857, SingaporeProgramme in Emerging Infectious Diseases, Duke-NUS Medical School, 8 College Road, Singapore 169857, SingaporeProgramme in Emerging Infectious Diseases, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore; Singapore-MIT Alliance in Research and Technology, Antimicrobial Resistance Interdisciplinary Research Group, Singapore 138602, Singapore; Saw Swee Hock School of Public Health, National University of Singapore, Singapore 117549, Singapore; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore; Viral Research and Experimental Medicine Centre, SingHealth Duke-NUS Academic Medical Centre, Singapore 169856, SingaporeProgramme in Emerging Infectious Diseases, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore; Corresponding authorSummary: Aberrant cellular bioenergetics has detrimental consequences in host cells. For instance, pathogenic Zika virus strains can suppress mitochondria respiration and glycolytic functions, disrupting cellular bioenergetics that leads to apoptosis. Herein, we describe methods for flavivirus propagation, titering and infection, cell preparation, and procedures for mitochondrial and glycolytic stress tests. The protocol enables assessment of cellular respiration and glycolytic flux in flavivirus-infected cells.For complete details on the use and execution of this protocol, please refer to Yau et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166722001770Cell BiologyCell cultureCell-based AssaysMetabolismMicrobiologyMolecular Biology |
| spellingShingle | John Z.H. Low Clement Yau Summer L.X. Zhang Hwee Cheng Tan Eng Eong Ooi Kuan Rong Chan A protocol to assess cellular bioenergetics in flavivirus-infected cells STAR Protocols Cell Biology Cell culture Cell-based Assays Metabolism Microbiology Molecular Biology |
| title | A protocol to assess cellular bioenergetics in flavivirus-infected cells |
| title_full | A protocol to assess cellular bioenergetics in flavivirus-infected cells |
| title_fullStr | A protocol to assess cellular bioenergetics in flavivirus-infected cells |
| title_full_unstemmed | A protocol to assess cellular bioenergetics in flavivirus-infected cells |
| title_short | A protocol to assess cellular bioenergetics in flavivirus-infected cells |
| title_sort | protocol to assess cellular bioenergetics in flavivirus infected cells |
| topic | Cell Biology Cell culture Cell-based Assays Metabolism Microbiology Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722001770 |
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