Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients
Laccases can oxidize a wide range of aromatic compounds and are industrially valuable. Laccases often exist in gene families and may differ from each other in expression and function. Quantitative real-time polymerase chain reaction (qPCR) was used for transcription profiling of eight laccase genes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01558/full |
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| author | Jie eYang Jie eYang Guozeng eWang Guozeng eWang Tzi Bun eNg Juan eLin Juan eLin Xiuyun eYe Xiuyun eYe |
| author_facet | Jie eYang Jie eYang Guozeng eWang Guozeng eWang Tzi Bun eNg Juan eLin Juan eLin Xiuyun eYe Xiuyun eYe |
| author_sort | Jie eYang |
| collection | DOAJ |
| description | Laccases can oxidize a wide range of aromatic compounds and are industrially valuable. Laccases often exist in gene families and may differ from each other in expression and function. Quantitative real-time polymerase chain reaction (qPCR) was used for transcription profiling of eight laccase genes in Cerrena sp. strain HYB07 with validated reference genes. A high laccase activity of 280.0 U/mL was obtained after submerged fermentation for 5 d. Laccase production and laccase gene transcription at different fermentation stages and in response to various environmental cues were revealed. HYB07 laccase activity correlated with transcription levels of its predominantly expressed laccase gene, Lac7. Cu2+ ions were indispensible for efficient laccase production by HYB07, mainly through Lac7 transcription induction, and no aromatic compounds were needed. HYB07 laccase synthesis and biomass accumulation were highest with non-limiting carbon and nitrogen. Glycerol and inorganic nitrogen sources adversely impacted Lac7 transcription, laccase yields and fungal growth. The present study would further our understanding of transcription regulation of laccase genes, which may in turn facilitate laccase production as well as elucidation of their physiological roles. |
| first_indexed | 2024-12-12T15:17:44Z |
| format | Article |
| id | doaj.art-23b6c779b4dc4a629b20921c37ecc5f3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T15:17:44Z |
| publishDate | 2016-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-23b6c779b4dc4a629b20921c37ecc5f32022-12-22T00:20:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-01-01610.3389/fmicb.2015.01558173464Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrientsJie eYang0Jie eYang1Guozeng eWang2Guozeng eWang3Tzi Bun eNg4Juan eLin5Juan eLin6Xiuyun eYe7Xiuyun eYe8Fuzhou UniversityFujian Key Laboratory of Marine Enzyme EngineeringFuzhou UniversityFujian Key Laboratory of Marine Enzyme EngineeringThe Chinese University of Hong KongFuzhou UniversityFujian Key Laboratory of Marine Enzyme EngineeringFuzhou UniversityFujian Key Laboratory of Marine Enzyme EngineeringLaccases can oxidize a wide range of aromatic compounds and are industrially valuable. Laccases often exist in gene families and may differ from each other in expression and function. Quantitative real-time polymerase chain reaction (qPCR) was used for transcription profiling of eight laccase genes in Cerrena sp. strain HYB07 with validated reference genes. A high laccase activity of 280.0 U/mL was obtained after submerged fermentation for 5 d. Laccase production and laccase gene transcription at different fermentation stages and in response to various environmental cues were revealed. HYB07 laccase activity correlated with transcription levels of its predominantly expressed laccase gene, Lac7. Cu2+ ions were indispensible for efficient laccase production by HYB07, mainly through Lac7 transcription induction, and no aromatic compounds were needed. HYB07 laccase synthesis and biomass accumulation were highest with non-limiting carbon and nitrogen. Glycerol and inorganic nitrogen sources adversely impacted Lac7 transcription, laccase yields and fungal growth. The present study would further our understanding of transcription regulation of laccase genes, which may in turn facilitate laccase production as well as elucidation of their physiological roles.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01558/fullLaccaseqPCRPromoterdifferential regulationCerrena sp. |
| spellingShingle | Jie eYang Jie eYang Guozeng eWang Guozeng eWang Tzi Bun eNg Juan eLin Juan eLin Xiuyun eYe Xiuyun eYe Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients Frontiers in Microbiology Laccase qPCR Promoter differential regulation Cerrena sp. |
| title | Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients |
| title_full | Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients |
| title_fullStr | Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients |
| title_full_unstemmed | Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients |
| title_short | Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients |
| title_sort | laccase production and differential transcription of laccase genes in cerrena sp in response to metal ions aromatic compounds and nutrients |
| topic | Laccase qPCR Promoter differential regulation Cerrena sp. |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01558/full |
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