Evidence for paternal DNA transmission to gynogenetic grass carp
Abstract Background Grass carp (Ctenopharyngodon idellus, GC), as the highest-output fish in China, is economically important. The production of gynogenetic grass carp (GGC) will provide important germplasm resource for producing improved GC. At present, knowledge regarding the heterologous sperm DN...
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| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Genetics |
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| Online Access: | http://link.springer.com/article/10.1186/s12863-018-0712-x |
| _version_ | 1828395193231998976 |
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| author | Zhuangwen Mao Yeqing Fu Yude Wang Shi Wang Minghe Zhang Xin Gao Kaikun Luo Qinbo Qin Chun Zhang Min Tao Zhanzhou Yao Shaojun Liu |
| author_facet | Zhuangwen Mao Yeqing Fu Yude Wang Shi Wang Minghe Zhang Xin Gao Kaikun Luo Qinbo Qin Chun Zhang Min Tao Zhanzhou Yao Shaojun Liu |
| author_sort | Zhuangwen Mao |
| collection | DOAJ |
| description | Abstract Background Grass carp (Ctenopharyngodon idellus, GC), as the highest-output fish in China, is economically important. The production of gynogenetic grass carp (GGC) will provide important germplasm resource for producing improved GC. At present, knowledge regarding the heterologous sperm DNA in gynogenetic offspring is little. Thus, revealing paternal DNA in GGC at the molecular level would be highly significant for fish genetic breeding. Result In this study, ultraviolet-treated sperm of koi carp (Cyprinus carpio haematopterus, KOC, 2n = 100), was used to activate the eggs of GC (2n = 48). Afterwards, cold shock (0–4 °C) was administered for 12 min to double the chromosomes, resulting in GGC. No significant difference (p > 0.05) was found between GGC and GC in appearance, erythrocytes size and chromosome numbers. However, at the molecular level, a specific microsatellite DNA fragment (MFW1-gynogenetic grass carp, MFW1-G) derived from the paternal parent KOC was found to be transmitted into GGC. Conclusions For the first time, this study provided an evidence at the molecular level that the DNA fragment derived from the paternal parent occurred in GGC. This finding is of great significance for fish genetic breeding. |
| first_indexed | 2024-12-10T08:08:35Z |
| format | Article |
| id | doaj.art-23b899983b7e4cbd80c982c10dce27d2 |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-12-10T08:08:35Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-23b899983b7e4cbd80c982c10dce27d22022-12-22T01:56:37ZengBMCBMC Genetics1471-21562019-01-012011710.1186/s12863-018-0712-xEvidence for paternal DNA transmission to gynogenetic grass carpZhuangwen Mao0Yeqing Fu1Yude Wang2Shi Wang3Minghe Zhang4Xin Gao5Kaikun Luo6Qinbo Qin7Chun Zhang8Min Tao9Zhanzhou Yao10Shaojun Liu11State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal UniversityAbstract Background Grass carp (Ctenopharyngodon idellus, GC), as the highest-output fish in China, is economically important. The production of gynogenetic grass carp (GGC) will provide important germplasm resource for producing improved GC. At present, knowledge regarding the heterologous sperm DNA in gynogenetic offspring is little. Thus, revealing paternal DNA in GGC at the molecular level would be highly significant for fish genetic breeding. Result In this study, ultraviolet-treated sperm of koi carp (Cyprinus carpio haematopterus, KOC, 2n = 100), was used to activate the eggs of GC (2n = 48). Afterwards, cold shock (0–4 °C) was administered for 12 min to double the chromosomes, resulting in GGC. No significant difference (p > 0.05) was found between GGC and GC in appearance, erythrocytes size and chromosome numbers. However, at the molecular level, a specific microsatellite DNA fragment (MFW1-gynogenetic grass carp, MFW1-G) derived from the paternal parent KOC was found to be transmitted into GGC. Conclusions For the first time, this study provided an evidence at the molecular level that the DNA fragment derived from the paternal parent occurred in GGC. This finding is of great significance for fish genetic breeding.http://link.springer.com/article/10.1186/s12863-018-0712-xCtenopharyngodon idellusCyprinus carpio haematopterusGynogenesisMicrosatellite DNADNA fragment |
| spellingShingle | Zhuangwen Mao Yeqing Fu Yude Wang Shi Wang Minghe Zhang Xin Gao Kaikun Luo Qinbo Qin Chun Zhang Min Tao Zhanzhou Yao Shaojun Liu Evidence for paternal DNA transmission to gynogenetic grass carp BMC Genetics Ctenopharyngodon idellus Cyprinus carpio haematopterus Gynogenesis Microsatellite DNA DNA fragment |
| title | Evidence for paternal DNA transmission to gynogenetic grass carp |
| title_full | Evidence for paternal DNA transmission to gynogenetic grass carp |
| title_fullStr | Evidence for paternal DNA transmission to gynogenetic grass carp |
| title_full_unstemmed | Evidence for paternal DNA transmission to gynogenetic grass carp |
| title_short | Evidence for paternal DNA transmission to gynogenetic grass carp |
| title_sort | evidence for paternal dna transmission to gynogenetic grass carp |
| topic | Ctenopharyngodon idellus Cyprinus carpio haematopterus Gynogenesis Microsatellite DNA DNA fragment |
| url | http://link.springer.com/article/10.1186/s12863-018-0712-x |
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