Taisui TS-2007S, a Large Microbial Mat Discovered in Soil in China
In this study, Taisui TS-2007S, a previously unidentified biological object discovered in soil in China, was identified. TS-2007S was shown to contain abundant carbohydrates but a scarcity of protein, fat, and minerals. The exopolymers of TS-2007S showed FT-IR spectra that were similar to those of x...
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| Format: | Article |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.592034/full |
| _version_ | 1828468551325843456 |
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| author | Tongfu Su Haohao Liu Chaohui Zhang Di Shang Chaojiang Wang Liyou Qiu |
| author_facet | Tongfu Su Haohao Liu Chaohui Zhang Di Shang Chaojiang Wang Liyou Qiu |
| author_sort | Tongfu Su |
| collection | DOAJ |
| description | In this study, Taisui TS-2007S, a previously unidentified biological object discovered in soil in China, was identified. TS-2007S was shown to contain abundant carbohydrates but a scarcity of protein, fat, and minerals. The exopolymers of TS-2007S showed FT-IR spectra that were similar to those of xanthan gum (XG) but that were dissimilar to those of polyvinyl alcohol (PVA). The NMR spectra of TS-2007S exopolymers in D2O were similar to those of PVA but differed from those of xanthan gum. Unlike PVA, TS-2007S exopolymers and xanthan gum were not soluble in dimethyl sulfoxide (DMSO). Furthermore, the exopolymers contained many monosaccharide components, including fucose, rhamnose, mannose, and glucuronic acid in a molar ratio of 87.90:7.49:4.45:0.15. The exopolymers also included traces of glucuronic acid, galactose, and xylose. Taken together, these results suggest that the exopolymers are microbial extracellular polymeric substances (EPSs). The microbial community structure in TS-2007S showed that the predominant bacterial, archaeal, and fungal phyla were Proteobacteria, Euryarchaeota, and Ascomycota at high relative abundances of 90.77, 97.15, and 87.43%, respectively, different from those observed in water and soil environments. Based on these results, we strongly propose that TS-2007S should be defined as a microbial mat formed in soil. |
| first_indexed | 2024-12-11T04:25:22Z |
| format | Article |
| id | doaj.art-23b9ef1abce84adcb957cc6bc0019b92 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T04:25:22Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-23b9ef1abce84adcb957cc6bc0019b922022-12-22T01:21:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-11-011110.3389/fmicb.2020.592034592034Taisui TS-2007S, a Large Microbial Mat Discovered in Soil in ChinaTongfu Su0Haohao Liu1Chaohui Zhang2Di Shang3Chaojiang Wang4Liyou Qiu5College of Sciences, Henan Agricultural University, Zhengzhou, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou, ChinaCollege of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou, ChinaInstitute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou, ChinaIn this study, Taisui TS-2007S, a previously unidentified biological object discovered in soil in China, was identified. TS-2007S was shown to contain abundant carbohydrates but a scarcity of protein, fat, and minerals. The exopolymers of TS-2007S showed FT-IR spectra that were similar to those of xanthan gum (XG) but that were dissimilar to those of polyvinyl alcohol (PVA). The NMR spectra of TS-2007S exopolymers in D2O were similar to those of PVA but differed from those of xanthan gum. Unlike PVA, TS-2007S exopolymers and xanthan gum were not soluble in dimethyl sulfoxide (DMSO). Furthermore, the exopolymers contained many monosaccharide components, including fucose, rhamnose, mannose, and glucuronic acid in a molar ratio of 87.90:7.49:4.45:0.15. The exopolymers also included traces of glucuronic acid, galactose, and xylose. Taken together, these results suggest that the exopolymers are microbial extracellular polymeric substances (EPSs). The microbial community structure in TS-2007S showed that the predominant bacterial, archaeal, and fungal phyla were Proteobacteria, Euryarchaeota, and Ascomycota at high relative abundances of 90.77, 97.15, and 87.43%, respectively, different from those observed in water and soil environments. Based on these results, we strongly propose that TS-2007S should be defined as a microbial mat formed in soil.https://www.frontiersin.org/articles/10.3389/fmicb.2020.592034/fullmicrobial matsextracellular polymeric substanceschemical compositionspectra analysismicrobial community structureTaisui |
| spellingShingle | Tongfu Su Haohao Liu Chaohui Zhang Di Shang Chaojiang Wang Liyou Qiu Taisui TS-2007S, a Large Microbial Mat Discovered in Soil in China Frontiers in Microbiology microbial mats extracellular polymeric substances chemical composition spectra analysis microbial community structure Taisui |
| title | Taisui TS-2007S, a Large Microbial Mat Discovered in Soil in China |
| title_full | Taisui TS-2007S, a Large Microbial Mat Discovered in Soil in China |
| title_fullStr | Taisui TS-2007S, a Large Microbial Mat Discovered in Soil in China |
| title_full_unstemmed | Taisui TS-2007S, a Large Microbial Mat Discovered in Soil in China |
| title_short | Taisui TS-2007S, a Large Microbial Mat Discovered in Soil in China |
| title_sort | taisui ts 2007s a large microbial mat discovered in soil in china |
| topic | microbial mats extracellular polymeric substances chemical composition spectra analysis microbial community structure Taisui |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.592034/full |
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