INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO
Effects of in vitro fertilization (IVF) temperature andcumulus oophorus cells removal after IVF or 12 h of embryoculture (IVC) on polyspermy and embryo development rates wereevaluated in swine. Oocytes and spermatozoa were incubated at37 or 38.5ºC during IVF procedure. Cumulus oophorus cells wererem...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Goiás
2010-06-01
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| Series: | Ciência Animal Brasileira |
| Subjects: | |
| Online Access: | http://www.revistas.ufg.br/index.php/vet/article/view/3402/6833 |
| Summary: | Effects of in vitro fertilization (IVF) temperature andcumulus oophorus cells removal after IVF or 12 h of embryoculture (IVC) on polyspermy and embryo development rates wereevaluated in swine. Oocytes and spermatozoa were incubated at37 or 38.5ºC during IVF procedure. Cumulus oophorus cells wereremoved from 50% of zygotes of each group 8 hours after IVF andall zygotes were cultured with NCSU23 media. Polyspermy rateswere assessed after 12 hours of IVC, when cumulus oophorus cellswere removed from the rest of zygotes. In a second experiment,embryos remained in culture for the evaluation of embryodevelopment. No effects of IVF temperature or cumulus oophoruscells removal were observed after 12 hours of IVC on polyspermyand embryo development (p<0.05). In conclusion, IVF temperatureand the presence of cumulus oophorus cells after IVF do notinterfere on polyspermy and embryo development rates. |
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| ISSN: | 1518-2797 1089-6891 |