INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO
Effects of in vitro fertilization (IVF) temperature andcumulus oophorus cells removal after IVF or 12 h of embryoculture (IVC) on polyspermy and embryo development rates wereevaluated in swine. Oocytes and spermatozoa were incubated at37 or 38.5ºC during IVF procedure. Cumulus oophorus cells wererem...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2010-06-01
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| Series: | Ciência Animal Brasileira |
| Subjects: | |
| Online Access: | http://www.revistas.ufg.br/index.php/vet/article/view/3402/6833 |
| _version_ | 1818265331525746688 |
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| author | Melissa Savoia Castilho Cunha Mariana Groke Marques Alecsandra Sobreira Lima Alessandra Corallo Nicacio Mayra Elena Ortiz Davila Assumpção José Antonio Visintin |
| author_facet | Melissa Savoia Castilho Cunha Mariana Groke Marques Alecsandra Sobreira Lima Alessandra Corallo Nicacio Mayra Elena Ortiz Davila Assumpção José Antonio Visintin |
| author_sort | Melissa Savoia Castilho Cunha |
| collection | DOAJ |
| description | Effects of in vitro fertilization (IVF) temperature andcumulus oophorus cells removal after IVF or 12 h of embryoculture (IVC) on polyspermy and embryo development rates wereevaluated in swine. Oocytes and spermatozoa were incubated at37 or 38.5ºC during IVF procedure. Cumulus oophorus cells wereremoved from 50% of zygotes of each group 8 hours after IVF andall zygotes were cultured with NCSU23 media. Polyspermy rateswere assessed after 12 hours of IVC, when cumulus oophorus cellswere removed from the rest of zygotes. In a second experiment,embryos remained in culture for the evaluation of embryodevelopment. No effects of IVF temperature or cumulus oophoruscells removal were observed after 12 hours of IVC on polyspermyand embryo development (p<0.05). In conclusion, IVF temperatureand the presence of cumulus oophorus cells after IVF do notinterfere on polyspermy and embryo development rates. |
| first_indexed | 2024-12-12T19:49:07Z |
| format | Article |
| id | doaj.art-23ba128b3f9e4b6097832a6dc902f9b0 |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1089-6891 |
| language | English |
| last_indexed | 2024-12-12T19:49:07Z |
| publishDate | 2010-06-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-23ba128b3f9e4b6097832a6dc902f9b02022-12-22T00:14:00ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971089-68912010-06-01112301306INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIOMelissa Savoia Castilho CunhaMariana Groke MarquesAlecsandra Sobreira LimaAlessandra Corallo NicacioMayra Elena Ortiz Davila AssumpçãoJosé Antonio VisintinEffects of in vitro fertilization (IVF) temperature andcumulus oophorus cells removal after IVF or 12 h of embryoculture (IVC) on polyspermy and embryo development rates wereevaluated in swine. Oocytes and spermatozoa were incubated at37 or 38.5ºC during IVF procedure. Cumulus oophorus cells wereremoved from 50% of zygotes of each group 8 hours after IVF andall zygotes were cultured with NCSU23 media. Polyspermy rateswere assessed after 12 hours of IVC, when cumulus oophorus cellswere removed from the rest of zygotes. In a second experiment,embryos remained in culture for the evaluation of embryodevelopment. No effects of IVF temperature or cumulus oophoruscells removal were observed after 12 hours of IVC on polyspermyand embryo development (p<0.05). In conclusion, IVF temperatureand the presence of cumulus oophorus cells after IVF do notinterfere on polyspermy and embryo development rates.http://www.revistas.ufg.br/index.php/vet/article/view/3402/6833Cumulus oophorus cellsin vitro fertilizationpolyspermyswinetemperature |
| spellingShingle | Melissa Savoia Castilho Cunha Mariana Groke Marques Alecsandra Sobreira Lima Alessandra Corallo Nicacio Mayra Elena Ortiz Davila Assumpção José Antonio Visintin INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO Ciência Animal Brasileira Cumulus oophorus cells in vitro fertilization polyspermy swine temperature |
| title | INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO |
| title_full | INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO |
| title_fullStr | INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO |
| title_full_unstemmed | INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO |
| title_short | INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO |
| title_sort | influencia da temperatura de fecundacao in vitro e das celulas do cumulus oophorus sobre a taxa de polispermia e desenvolvimento embrionario |
| topic | Cumulus oophorus cells in vitro fertilization polyspermy swine temperature |
| url | http://www.revistas.ufg.br/index.php/vet/article/view/3402/6833 |
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