Vibrio parahaemolyticus and Salmonella enterica isolates in fish species captured from the Lagoa dos Patos estuary
Microorganisms that cause human diseases can contaminate fishes in aquatic environments as well as during their capture, handling, and transport. The purpose of this study was to isolate Vibrio parahaemolyticus and Salmonella enterica from fishes captured in the Lagoa dos Patos estuary that were evi...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2016-06-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21540 |
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| author | Janaina Viana da Rosa Carolina Janelli da Silva Franciele Barbosa Jacqueline Bairros Eduarda Hallal Duval Elizabete Helbig Cláudio Dias Timm |
| author_facet | Janaina Viana da Rosa Carolina Janelli da Silva Franciele Barbosa Jacqueline Bairros Eduarda Hallal Duval Elizabete Helbig Cláudio Dias Timm |
| author_sort | Janaina Viana da Rosa |
| collection | DOAJ |
| description | Microorganisms that cause human diseases can contaminate fishes in aquatic environments as well as during their capture, handling, and transport. The purpose of this study was to isolate Vibrio parahaemolyticus and Salmonella enterica from fishes captured in the Lagoa dos Patos estuary that were eviscerated and cleaned for trade. Thirteen fish landing were analyzed and 65 entire fishes and 65 cleaned fishes were studied to determine the presence of V. parahaemolyticus and S. enterica. Bacterial isolates were compared using rep-PCR. V. parahaemolyticus was isolated from one entire Micropogonias furnieri and two entire Mugil platanus, as well as from three eviscerated M. platanus. S. enterica was isolated from two eviscerated Paralichthys orbignyanus. Identical rep-PCR bands from V. parahaemolyticus were observed in entire and eviscerated fishes from the same discharge, suggesting processing failures that neither eliminated the microorganism from the raw material nor prevented cross-contamination. S. enterica was not isolated from entire fishes, presumably because contamination occurred due to hygiene and sanitary failures. Our results showed that M. furnieri and M. platanus captured in the Lagoa dos Patos estuary may host V. parahaemolyticus and that this microorganism, as with S. enterica, may also persist even after the fish is cleaned. This is the first record of the isolation of V. parahaemolyticus from M. furnieri. |
| first_indexed | 2024-12-14T10:16:13Z |
| format | Article |
| id | doaj.art-23bb86d8107044bfbd5d6e7597a5e25e |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-14T10:16:13Z |
| publishDate | 2016-06-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-23bb86d8107044bfbd5d6e7597a5e25e2022-12-21T23:06:49ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592016-06-013731345135410.5433/1679-0359.2016v37n3p134513945Vibrio parahaemolyticus and Salmonella enterica isolates in fish species captured from the Lagoa dos Patos estuaryJanaina Viana da Rosa0Carolina Janelli da Silva1Franciele Barbosa2Jacqueline Bairros3Eduarda Hallal Duval4Elizabete Helbig5Cláudio Dias Timm6Universidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasMicroorganisms that cause human diseases can contaminate fishes in aquatic environments as well as during their capture, handling, and transport. The purpose of this study was to isolate Vibrio parahaemolyticus and Salmonella enterica from fishes captured in the Lagoa dos Patos estuary that were eviscerated and cleaned for trade. Thirteen fish landing were analyzed and 65 entire fishes and 65 cleaned fishes were studied to determine the presence of V. parahaemolyticus and S. enterica. Bacterial isolates were compared using rep-PCR. V. parahaemolyticus was isolated from one entire Micropogonias furnieri and two entire Mugil platanus, as well as from three eviscerated M. platanus. S. enterica was isolated from two eviscerated Paralichthys orbignyanus. Identical rep-PCR bands from V. parahaemolyticus were observed in entire and eviscerated fishes from the same discharge, suggesting processing failures that neither eliminated the microorganism from the raw material nor prevented cross-contamination. S. enterica was not isolated from entire fishes, presumably because contamination occurred due to hygiene and sanitary failures. Our results showed that M. furnieri and M. platanus captured in the Lagoa dos Patos estuary may host V. parahaemolyticus and that this microorganism, as with S. enterica, may also persist even after the fish is cleaned. This is the first record of the isolation of V. parahaemolyticus from M. furnieri.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21540Micro-organismos patogênicosMugil platanusParalichthys orbignyanusPeixeSegurança alimentar. |
| spellingShingle | Janaina Viana da Rosa Carolina Janelli da Silva Franciele Barbosa Jacqueline Bairros Eduarda Hallal Duval Elizabete Helbig Cláudio Dias Timm Vibrio parahaemolyticus and Salmonella enterica isolates in fish species captured from the Lagoa dos Patos estuary Semina: Ciências Agrárias Micro-organismos patogênicos Mugil platanus Paralichthys orbignyanus Peixe Segurança alimentar. |
| title | Vibrio parahaemolyticus and Salmonella enterica isolates in fish species captured from the Lagoa dos Patos estuary |
| title_full | Vibrio parahaemolyticus and Salmonella enterica isolates in fish species captured from the Lagoa dos Patos estuary |
| title_fullStr | Vibrio parahaemolyticus and Salmonella enterica isolates in fish species captured from the Lagoa dos Patos estuary |
| title_full_unstemmed | Vibrio parahaemolyticus and Salmonella enterica isolates in fish species captured from the Lagoa dos Patos estuary |
| title_short | Vibrio parahaemolyticus and Salmonella enterica isolates in fish species captured from the Lagoa dos Patos estuary |
| title_sort | vibrio parahaemolyticus and salmonella enterica isolates in fish species captured from the lagoa dos patos estuary |
| topic | Micro-organismos patogênicos Mugil platanus Paralichthys orbignyanus Peixe Segurança alimentar. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21540 |
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