Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals
The purpose of the present study was to evaluate the sexual transmission of GBV-C/HGV, through RNA detection in cervicovaginal smears. Therefore the GBV-C/HGV RNA in cervicovaginal smears from apparently healthy women was investigated using routine proceedings for prophylactic screening to cervical...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2008-10-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500007 |
| _version_ | 1819101363488948224 |
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| author | Maria Angelica Ehara Watanabe Ana Karina Melim Benthien Miquelão Carlos Eduardo Coral de Oliveira Karen Brajão de Oliveira Thiago Franco Nasser Mateus Nóbrega Aoki Patrícia Sayuri Suzuki Marla Karine Amarante Emerson José Venâncio Elbens Marcos Minoreli de Azevedo |
| author_facet | Maria Angelica Ehara Watanabe Ana Karina Melim Benthien Miquelão Carlos Eduardo Coral de Oliveira Karen Brajão de Oliveira Thiago Franco Nasser Mateus Nóbrega Aoki Patrícia Sayuri Suzuki Marla Karine Amarante Emerson José Venâncio Elbens Marcos Minoreli de Azevedo |
| author_sort | Maria Angelica Ehara Watanabe |
| collection | DOAJ |
| description | The purpose of the present study was to evaluate the sexual transmission of GBV-C/HGV, through RNA detection in cervicovaginal smears. Therefore the GBV-C/HGV RNA in cervicovaginal smears from apparently healthy women was investigated using routine proceedings for prophylactic screening to cervical cancer. GBV-C/HGV RNA was detected by reverse transcriptase and polymerase chain reaction (RT-PCR). Only one woman presented co-infection with human papilloma virus (HPV). The GBV-C/HGV RNA was detected in 13/73 (17.57%) healthy women and it's prevalence in participating women between 28-43 years old was 53.85%. No association was found with GBV-C/HGV for the age of first sexual intercourse and number of pregnancies. In GBV-C/HGV RNA positive women, 69.23% were married. In conclusion, the present findings show that cervical and vaginal specimens could contain the GBV-C/HGV RNA.<br>O objetivo do presente estudo foi avaliar a transmissão sexual de GBV-C/HBV, através da detecção do RNA viral em raspados cérvico-vaginais. Portanto, a presença do RNA GBV-C/HGV foi investigada em raspados cérvico-vaginais em mulheres aparentemente saudáveis que realizaram exames preventivos para câncer cervical. GBV-C/HGV RNA foi detectado por reação de transcriptase reversa e reação em cadeia da polimerase (RT-PCR). Apenas uma mulher apresentou a co-infecção com o papiloma vírus humano (HPV). O RNA GBV-C/HGV foi detectado em 13/73 (17,57%) mulheres saudáveis e sua prevalência entre participantes da idade de 28-43 anos foi de 53,85%. Não foi observada relação entre a presença do RNA GBV-C/HGV com a idade de primeira relação sexual, nem com o número de gestações. Entre as mulheres que apresentavam o RNA viral, 69,23% eram casadas. O presente estudo demonstrou que secreções cérvico-vaginais podem conter o RNA viral GBV-C/HBV. |
| first_indexed | 2024-12-22T01:17:29Z |
| format | Article |
| id | doaj.art-23bd61bbfa9e499abd8a09d499e58d49 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-22T01:17:29Z |
| publishDate | 2008-10-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-23bd61bbfa9e499abd8a09d499e58d492022-12-21T18:43:49ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242008-10-0151591792210.1590/S1516-89132008000500007Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individualsMaria Angelica Ehara WatanabeAna Karina Melim Benthien MiquelãoCarlos Eduardo Coral de OliveiraKaren Brajão de OliveiraThiago Franco NasserMateus Nóbrega AokiPatrícia Sayuri SuzukiMarla Karine AmaranteEmerson José VenâncioElbens Marcos Minoreli de AzevedoThe purpose of the present study was to evaluate the sexual transmission of GBV-C/HGV, through RNA detection in cervicovaginal smears. Therefore the GBV-C/HGV RNA in cervicovaginal smears from apparently healthy women was investigated using routine proceedings for prophylactic screening to cervical cancer. GBV-C/HGV RNA was detected by reverse transcriptase and polymerase chain reaction (RT-PCR). Only one woman presented co-infection with human papilloma virus (HPV). The GBV-C/HGV RNA was detected in 13/73 (17.57%) healthy women and it's prevalence in participating women between 28-43 years old was 53.85%. No association was found with GBV-C/HGV for the age of first sexual intercourse and number of pregnancies. In GBV-C/HGV RNA positive women, 69.23% were married. In conclusion, the present findings show that cervical and vaginal specimens could contain the GBV-C/HGV RNA.<br>O objetivo do presente estudo foi avaliar a transmissão sexual de GBV-C/HBV, através da detecção do RNA viral em raspados cérvico-vaginais. Portanto, a presença do RNA GBV-C/HGV foi investigada em raspados cérvico-vaginais em mulheres aparentemente saudáveis que realizaram exames preventivos para câncer cervical. GBV-C/HGV RNA foi detectado por reação de transcriptase reversa e reação em cadeia da polimerase (RT-PCR). Apenas uma mulher apresentou a co-infecção com o papiloma vírus humano (HPV). O RNA GBV-C/HGV foi detectado em 13/73 (17,57%) mulheres saudáveis e sua prevalência entre participantes da idade de 28-43 anos foi de 53,85%. Não foi observada relação entre a presença do RNA GBV-C/HGV com a idade de primeira relação sexual, nem com o número de gestações. Entre as mulheres que apresentavam o RNA viral, 69,23% eram casadas. O presente estudo demonstrou que secreções cérvico-vaginais podem conter o RNA viral GBV-C/HBV.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500007GBV-C/HGV RNAhealthy womencervicovaginal smears |
| spellingShingle | Maria Angelica Ehara Watanabe Ana Karina Melim Benthien Miquelão Carlos Eduardo Coral de Oliveira Karen Brajão de Oliveira Thiago Franco Nasser Mateus Nóbrega Aoki Patrícia Sayuri Suzuki Marla Karine Amarante Emerson José Venâncio Elbens Marcos Minoreli de Azevedo Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals Brazilian Archives of Biology and Technology GBV-C/HGV RNA healthy women cervicovaginal smears |
| title | Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals |
| title_full | Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals |
| title_fullStr | Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals |
| title_full_unstemmed | Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals |
| title_short | Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals |
| title_sort | detection of gbv c hgv rna in cervico vaginal smears from healthy individuals |
| topic | GBV-C/HGV RNA healthy women cervicovaginal smears |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500007 |
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