Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in Haiti
Abstract Background Malaria continues to cause burden in various parts of the world. Haiti, a Caribbean country, is among those aiming to eliminate malaria within a few years. Two surveys were conducted in Haiti during which we aimed to evaluate the performance of the simple and rapid procedure for...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-05-01
|
| Series: | Infectious Diseases of Poverty |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40249-023-01097-w |
| _version_ | 1797817687149117440 |
|---|---|
| author | Jeanne Perpétue Vincent Alexandre Valcena Existe Kanako Komaki-Yasuda Jacques Boncy Shigeyuki Kano |
| author_facet | Jeanne Perpétue Vincent Alexandre Valcena Existe Kanako Komaki-Yasuda Jacques Boncy Shigeyuki Kano |
| author_sort | Jeanne Perpétue Vincent |
| collection | DOAJ |
| description | Abstract Background Malaria continues to cause burden in various parts of the world. Haiti, a Caribbean country, is among those aiming to eliminate malaria within a few years. Two surveys were conducted in Haiti during which we aimed to evaluate the performance of the simple and rapid procedure for ultra-rapid extraction–loop-mediated isothermal amplification (PURE-LAMP) method with dried blood spots as an alternative diagnostic method for malaria in the context of low to very low rates of transmission. Methods Febrile and afebrile people were recruited from three administrative divisions within Haiti: Nippes, Sud and Grand’Anse, during the summers of 2017 (early August to early September) and 2018 (late July to late August). Their blood samples were tested by microscopy, rapid diagnostic tests (RDT), PURE-LAMP and nested PCR to detect Plasmodium infection. Sensitivity, specificity, positive and negative predictive values and kappa statistics were estimated with the nested PCR results as the gold standard. Results Among 1074 samples analyzed, a positive rate of 8.3% was calculated based on the nested PCR results. Among febrile participants, the rates in 2017 and 2018 were 14.6% and 1.4%, respectively. Three positives were detected among 172 afebrile participants in 2018 by PURE-LAMP and nested PCR, and all three were from the same locality. There was no afebrile participants recruited in 2017. The PURE-LAMP, RDT and microscopy had respective sensitivities of 100%, 85.4% and 49.4%. All of the testing methods had specificities over 99%. Conclusions This study confirmed the high performance of the PURE-LAMP method to detect Plasmodium infection with dried blood spots and recommends its use in targeted mass screening and treatment activities in low endemic areas of malaria. |
| first_indexed | 2024-03-13T08:57:07Z |
| format | Article |
| id | doaj.art-23beb1b4f8124ed2bd30666dff0acfab |
| institution | Directory Open Access Journal |
| issn | 2049-9957 |
| language | English |
| last_indexed | 2024-03-13T08:57:07Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Diseases of Poverty |
| spelling | doaj.art-23beb1b4f8124ed2bd30666dff0acfab2023-05-28T11:31:02ZengBMCInfectious Diseases of Poverty2049-99572023-05-011211910.1186/s40249-023-01097-wPerformance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in HaitiJeanne Perpétue Vincent0Alexandre Valcena Existe1Kanako Komaki-Yasuda2Jacques Boncy3Shigeyuki Kano4Department of Tropical Medicine and Malaria, Research Institute, National Center for Global Health and MedicineLaboratoire National de Santé PubliqueDepartment of Tropical Medicine and Malaria, Research Institute, National Center for Global Health and MedicineLaboratoire National de Santé PubliqueDepartment of Tropical Medicine and Malaria, Research Institute, National Center for Global Health and MedicineAbstract Background Malaria continues to cause burden in various parts of the world. Haiti, a Caribbean country, is among those aiming to eliminate malaria within a few years. Two surveys were conducted in Haiti during which we aimed to evaluate the performance of the simple and rapid procedure for ultra-rapid extraction–loop-mediated isothermal amplification (PURE-LAMP) method with dried blood spots as an alternative diagnostic method for malaria in the context of low to very low rates of transmission. Methods Febrile and afebrile people were recruited from three administrative divisions within Haiti: Nippes, Sud and Grand’Anse, during the summers of 2017 (early August to early September) and 2018 (late July to late August). Their blood samples were tested by microscopy, rapid diagnostic tests (RDT), PURE-LAMP and nested PCR to detect Plasmodium infection. Sensitivity, specificity, positive and negative predictive values and kappa statistics were estimated with the nested PCR results as the gold standard. Results Among 1074 samples analyzed, a positive rate of 8.3% was calculated based on the nested PCR results. Among febrile participants, the rates in 2017 and 2018 were 14.6% and 1.4%, respectively. Three positives were detected among 172 afebrile participants in 2018 by PURE-LAMP and nested PCR, and all three were from the same locality. There was no afebrile participants recruited in 2017. The PURE-LAMP, RDT and microscopy had respective sensitivities of 100%, 85.4% and 49.4%. All of the testing methods had specificities over 99%. Conclusions This study confirmed the high performance of the PURE-LAMP method to detect Plasmodium infection with dried blood spots and recommends its use in targeted mass screening and treatment activities in low endemic areas of malaria.https://doi.org/10.1186/s40249-023-01097-wDiagnosisDried blood spotsHaitiLoop-mediated isothermal amplification (LAMP)Low transmissionMalaria |
| spellingShingle | Jeanne Perpétue Vincent Alexandre Valcena Existe Kanako Komaki-Yasuda Jacques Boncy Shigeyuki Kano Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in Haiti Infectious Diseases of Poverty Diagnosis Dried blood spots Haiti Loop-mediated isothermal amplification (LAMP) Low transmission Malaria |
| title | Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in Haiti |
| title_full | Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in Haiti |
| title_fullStr | Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in Haiti |
| title_full_unstemmed | Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in Haiti |
| title_short | Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in Haiti |
| title_sort | performance of the procedure for ultra rapid extraction and loop mediated isothermal amplification pure lamp method to detect malaria in haiti |
| topic | Diagnosis Dried blood spots Haiti Loop-mediated isothermal amplification (LAMP) Low transmission Malaria |
| url | https://doi.org/10.1186/s40249-023-01097-w |
| work_keys_str_mv | AT jeanneperpetuevincent performanceoftheprocedureforultrarapidextractionandloopmediatedisothermalamplificationpurelampmethodtodetectmalariainhaiti AT alexandrevalcenaexiste performanceoftheprocedureforultrarapidextractionandloopmediatedisothermalamplificationpurelampmethodtodetectmalariainhaiti AT kanakokomakiyasuda performanceoftheprocedureforultrarapidextractionandloopmediatedisothermalamplificationpurelampmethodtodetectmalariainhaiti AT jacquesboncy performanceoftheprocedureforultrarapidextractionandloopmediatedisothermalamplificationpurelampmethodtodetectmalariainhaiti AT shigeyukikano performanceoftheprocedureforultrarapidextractionandloopmediatedisothermalamplificationpurelampmethodtodetectmalariainhaiti |