Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complement
Schistosomula of Schistosoma mansoni became resistant to antibody-dependent complement damage in vitro after pre-incubation with normal human erythrocytes (NHuE) whatever the ABO or Rh blood group. Resistant parasites were shown to acquire host decay accelerating factor (DAF) , a 70 kDa glycoprotein...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1992-01-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000800016 |
| _version_ | 1797712655913320448 |
|---|---|
| author | F. Juarez Ramalho-Pinto Ermelinda M. R. D. Carvalho Maria de Fátima M. Horta |
| author_facet | F. Juarez Ramalho-Pinto Ermelinda M. R. D. Carvalho Maria de Fátima M. Horta |
| author_sort | F. Juarez Ramalho-Pinto |
| collection | DOAJ |
| description | Schistosomula of Schistosoma mansoni became resistant to antibody-dependent complement damage in vitro after pre-incubation with normal human erythrocytes (NHuE) whatever the ABO or Rh blood group. Resistant parasites were shown to acquire host decay accelerating factor (DAF) , a 70 kDa glycoprotein attached to the membrane of NHue by a GPI anchor. IgG2a mAb anti-human DAF (IA10) immunoprecipitated a 70 kDa molecule from 125I-labeled schistosomula pre-incubated with NHuE and inhibited their resistance to complement-dependent killing in vtro. Incubationof schistosomula with erytrocytes from patients with paroxsimal nocturnal hemoglobinuria (PNHE) or SRBC, wich are DAF-deficient, did not protect the parasites from complement lesion. Supernatant of 100,000 x g collected from NHuE incubated for 24 h in defined medium was shown to contain a soluble form of DAF and to protect schistosomula from complement killing. Schistosomula treated with trypsin before incubation with NHuE ghosts did not become resistant to complement damage. On the other hand, pre-treatment with chymotrypsin did not interfere with the acquisition of resistance by the schistosomula. These results indicate that, in vitro, NHuE DAF can be transferred to schistosomula in a soluble form and that the binding of this molecule to the parasite surface is dependent upon trypsin-sensitive chymotrypsin-insensitive polipeptide(s) present on the surface of the worm. |
| first_indexed | 2024-03-12T07:24:57Z |
| format | Article |
| id | doaj.art-23bf28b89fd8466ebeab6e958c9d4011 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:24:57Z |
| publishDate | 1992-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-23bf28b89fd8466ebeab6e958c9d40112023-09-02T22:10:42ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601992-01-018711111610.1590/S0074-02761992000800016Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complementF. Juarez Ramalho-PintoErmelinda M. R. D. CarvalhoMaria de Fátima M. HortaSchistosomula of Schistosoma mansoni became resistant to antibody-dependent complement damage in vitro after pre-incubation with normal human erythrocytes (NHuE) whatever the ABO or Rh blood group. Resistant parasites were shown to acquire host decay accelerating factor (DAF) , a 70 kDa glycoprotein attached to the membrane of NHue by a GPI anchor. IgG2a mAb anti-human DAF (IA10) immunoprecipitated a 70 kDa molecule from 125I-labeled schistosomula pre-incubated with NHuE and inhibited their resistance to complement-dependent killing in vtro. Incubationof schistosomula with erytrocytes from patients with paroxsimal nocturnal hemoglobinuria (PNHE) or SRBC, wich are DAF-deficient, did not protect the parasites from complement lesion. Supernatant of 100,000 x g collected from NHuE incubated for 24 h in defined medium was shown to contain a soluble form of DAF and to protect schistosomula from complement killing. Schistosomula treated with trypsin before incubation with NHuE ghosts did not become resistant to complement damage. On the other hand, pre-treatment with chymotrypsin did not interfere with the acquisition of resistance by the schistosomula. These results indicate that, in vitro, NHuE DAF can be transferred to schistosomula in a soluble form and that the binding of this molecule to the parasite surface is dependent upon trypsin-sensitive chymotrypsin-insensitive polipeptide(s) present on the surface of the worm.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000800016Schistosoma mansoni schistosomulacomplementhuman decay accelerating factor |
| spellingShingle | F. Juarez Ramalho-Pinto Ermelinda M. R. D. Carvalho Maria de Fátima M. Horta Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complement Memorias do Instituto Oswaldo Cruz Schistosoma mansoni schistosomula complement human decay accelerating factor |
| title | Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complement |
| title_full | Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complement |
| title_fullStr | Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complement |
| title_full_unstemmed | Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complement |
| title_short | Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complement |
| title_sort | mechanisms of evasion of schistosoma mansoni schistosomula to the lethal activity of complement |
| topic | Schistosoma mansoni schistosomula complement human decay accelerating factor |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000800016 |
| work_keys_str_mv | AT fjuarezramalhopinto mechanismsofevasionofschistosomamansonischistosomulatothelethalactivityofcomplement AT ermelindamrdcarvalho mechanismsofevasionofschistosomamansonischistosomulatothelethalactivityofcomplement AT mariadefatimamhorta mechanismsofevasionofschistosomamansonischistosomulatothelethalactivityofcomplement |