Circulating extracellular vesicle-derived MARCKSL1 is a potential diagnostic non-invasive biomarker in metastatic colorectal cancer patients
Abstract Extracellular vesicle-derived proteins are closely related to colorectal cancer metastasis, and early detection and diagnosis of colorectal cancer metastasis is very important to improve the prognosis. In this study, we evaluated the clinical significance of plasma EV-derived MARCKSL1 in di...
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| Format: | Article |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-37008-0 |
| _version_ | 1797795695693922304 |
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| author | Wenqing Rong Shiyun Shao Yunzhou Pu Qing Ji Huirong Zhu |
| author_facet | Wenqing Rong Shiyun Shao Yunzhou Pu Qing Ji Huirong Zhu |
| author_sort | Wenqing Rong |
| collection | DOAJ |
| description | Abstract Extracellular vesicle-derived proteins are closely related to colorectal cancer metastasis, and early detection and diagnosis of colorectal cancer metastasis is very important to improve the prognosis. In this study, we evaluated the clinical significance of plasma EV-derived MARCKSL1 in differentiating patients with metastatic and nonmetastatic CRC. This study included 78 patients, including 40 patients with nonmetastatic colorectal cancer, 38 patients with metastatic colorectal cancer, and 15 healthy volunteers. The extracellular vesicles extracted from the participants' plasma were characterized through transmission electron microscopy, nanoparticle tracking analysis and western blotting. MARCKSL1 protein expression in the EVs was detected by ELISA, and the diagnostic efficacy of MARCKSL1 alone or in combination with CA125 and lymphocyte levels was evaluated by receiver operating characteristic curve (ROC) analysis. Pearson's correlation test was performed to detect the correlation between MARCKSL1, CA125, lymphocyte level and clinicopathological characteristics of tumors. The present study demonstrated that the level of circulating EV-derived MARCKSL1 in patients with metastatic colorectal cancer was significantly higher than that in patients with nonmetastatic colorectal cancer and healthy people. Combined with CA125 and lymphocyte levels, the best diagnostic effect was achieved, and the area under the ROC curve was 0.7480. Together, our findings indicated that circulating EV-derived MARCKSL1 could be used as a new potential diagnostic biomarker for metastatic CRC. |
| first_indexed | 2024-03-13T03:21:54Z |
| format | Article |
| id | doaj.art-23c0d8bbb3984311aaa643f57adc60fb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T03:21:54Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-23c0d8bbb3984311aaa643f57adc60fb2023-06-25T11:15:49ZengNature PortfolioScientific Reports2045-23222023-06-0113111410.1038/s41598-023-37008-0Circulating extracellular vesicle-derived MARCKSL1 is a potential diagnostic non-invasive biomarker in metastatic colorectal cancer patientsWenqing Rong0Shiyun Shao1Yunzhou Pu2Qing Ji3Huirong Zhu4Department of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineAbstract Extracellular vesicle-derived proteins are closely related to colorectal cancer metastasis, and early detection and diagnosis of colorectal cancer metastasis is very important to improve the prognosis. In this study, we evaluated the clinical significance of plasma EV-derived MARCKSL1 in differentiating patients with metastatic and nonmetastatic CRC. This study included 78 patients, including 40 patients with nonmetastatic colorectal cancer, 38 patients with metastatic colorectal cancer, and 15 healthy volunteers. The extracellular vesicles extracted from the participants' plasma were characterized through transmission electron microscopy, nanoparticle tracking analysis and western blotting. MARCKSL1 protein expression in the EVs was detected by ELISA, and the diagnostic efficacy of MARCKSL1 alone or in combination with CA125 and lymphocyte levels was evaluated by receiver operating characteristic curve (ROC) analysis. Pearson's correlation test was performed to detect the correlation between MARCKSL1, CA125, lymphocyte level and clinicopathological characteristics of tumors. The present study demonstrated that the level of circulating EV-derived MARCKSL1 in patients with metastatic colorectal cancer was significantly higher than that in patients with nonmetastatic colorectal cancer and healthy people. Combined with CA125 and lymphocyte levels, the best diagnostic effect was achieved, and the area under the ROC curve was 0.7480. Together, our findings indicated that circulating EV-derived MARCKSL1 could be used as a new potential diagnostic biomarker for metastatic CRC.https://doi.org/10.1038/s41598-023-37008-0 |
| spellingShingle | Wenqing Rong Shiyun Shao Yunzhou Pu Qing Ji Huirong Zhu Circulating extracellular vesicle-derived MARCKSL1 is a potential diagnostic non-invasive biomarker in metastatic colorectal cancer patients Scientific Reports |
| title | Circulating extracellular vesicle-derived MARCKSL1 is a potential diagnostic non-invasive biomarker in metastatic colorectal cancer patients |
| title_full | Circulating extracellular vesicle-derived MARCKSL1 is a potential diagnostic non-invasive biomarker in metastatic colorectal cancer patients |
| title_fullStr | Circulating extracellular vesicle-derived MARCKSL1 is a potential diagnostic non-invasive biomarker in metastatic colorectal cancer patients |
| title_full_unstemmed | Circulating extracellular vesicle-derived MARCKSL1 is a potential diagnostic non-invasive biomarker in metastatic colorectal cancer patients |
| title_short | Circulating extracellular vesicle-derived MARCKSL1 is a potential diagnostic non-invasive biomarker in metastatic colorectal cancer patients |
| title_sort | circulating extracellular vesicle derived marcksl1 is a potential diagnostic non invasive biomarker in metastatic colorectal cancer patients |
| url | https://doi.org/10.1038/s41598-023-37008-0 |
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