Analysis of beauvericin in Isaria cicadae Miquel culture on different media

Objective To analyze beauvericin and enniatins concentrations in the culture complex after Isaria cicadae Miquel was inoculated on different media for certain time. Methods One strain of Isaria cicadae Miquel was inoculated on 4 kinds of liquid culture media and 4 kinds of solid culture media. After...

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Main Authors: Wenjing XU, Xiaomin HAN, Jing ZHANG, Fengqin LI
Format: Article
Language:zho
Published: The Editorial Office of Chinese Journal of Food Hygiene 2020-11-01
Series:Zhongguo shipin weisheng zazhi
Subjects:
Online Access:http://www.zgspws.com/zgspwszzen/article/abstract/20200604
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author Wenjing XU
Xiaomin HAN
Jing ZHANG
Fengqin LI
author_facet Wenjing XU
Xiaomin HAN
Jing ZHANG
Fengqin LI
author_sort Wenjing XU
collection DOAJ
description Objective To analyze beauvericin and enniatins concentrations in the culture complex after Isaria cicadae Miquel was inoculated on different media for certain time. Methods One strain of Isaria cicadae Miquel was inoculated on 4 kinds of liquid culture media and 4 kinds of solid culture media. After incubation for 1-7 weeks under 25 ℃, high performance liquid chromatography tandem mass spectrometry was employed to detect beauvericin and enniatins. Results The detection rates of beauvericin in Isaria cicadae Miquel culture on 4 kinds of liquid media ranged between 42.9%-100.0%, and the beauvericin concentrations varied from 1.0 to 94.6 μg/L. The positive rates of beauvericin in Isaria cicadae Miquel culture on 4 kinds of solid media were 100.0%, and the beauvericin concentrations ranged from 60.9 to 44 677.5 μg/kg. Enniatins were negative for Isaria cicadae Miquel on 8 kinds of culture media. Conclusion The strain of Isaria cicadae Miquel used in our study could produce beauvericin in 8 kinds of culture media after incubation for certain time, and the beauvericin concentrations in solid media were much higher than in liquid media. The strain of Isaria cicadae Miquel didn’t produce enniatins in 8 kinds of culture media after incubation for certain time.
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spelling doaj.art-23c18126ec444cd2a2c64459051430e22023-07-31T02:32:12ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562020-11-01320660961410.13590/j.cjfh.2020.06.0041004-8456(2020)06-0609-06Analysis of beauvericin in Isaria cicadae Miquel culture on different mediaWenjing XUXiaomin HANJing ZHANGFengqin LIObjective To analyze beauvericin and enniatins concentrations in the culture complex after Isaria cicadae Miquel was inoculated on different media for certain time. Methods One strain of Isaria cicadae Miquel was inoculated on 4 kinds of liquid culture media and 4 kinds of solid culture media. After incubation for 1-7 weeks under 25 ℃, high performance liquid chromatography tandem mass spectrometry was employed to detect beauvericin and enniatins. Results The detection rates of beauvericin in Isaria cicadae Miquel culture on 4 kinds of liquid media ranged between 42.9%-100.0%, and the beauvericin concentrations varied from 1.0 to 94.6 μg/L. The positive rates of beauvericin in Isaria cicadae Miquel culture on 4 kinds of solid media were 100.0%, and the beauvericin concentrations ranged from 60.9 to 44 677.5 μg/kg. Enniatins were negative for Isaria cicadae Miquel on 8 kinds of culture media. Conclusion The strain of Isaria cicadae Miquel used in our study could produce beauvericin in 8 kinds of culture media after incubation for certain time, and the beauvericin concentrations in solid media were much higher than in liquid media. The strain of Isaria cicadae Miquel didn’t produce enniatins in 8 kinds of culture media after incubation for certain time.http://www.zgspws.com/zgspwszzen/article/abstract/20200604isaria cicadae miquelbeauvericinenniatinshigh performance liquid chromatography-tandem mass spectrometrydetection
spellingShingle Wenjing XU
Xiaomin HAN
Jing ZHANG
Fengqin LI
Analysis of beauvericin in Isaria cicadae Miquel culture on different media
Zhongguo shipin weisheng zazhi
isaria cicadae miquel
beauvericin
enniatins
high performance liquid chromatography-tandem mass spectrometry
detection
title Analysis of beauvericin in Isaria cicadae Miquel culture on different media
title_full Analysis of beauvericin in Isaria cicadae Miquel culture on different media
title_fullStr Analysis of beauvericin in Isaria cicadae Miquel culture on different media
title_full_unstemmed Analysis of beauvericin in Isaria cicadae Miquel culture on different media
title_short Analysis of beauvericin in Isaria cicadae Miquel culture on different media
title_sort analysis of beauvericin in isaria cicadae miquel culture on different media
topic isaria cicadae miquel
beauvericin
enniatins
high performance liquid chromatography-tandem mass spectrometry
detection
url http://www.zgspws.com/zgspwszzen/article/abstract/20200604
work_keys_str_mv AT wenjingxu analysisofbeauvericininisariacicadaemiquelcultureondifferentmedia
AT xiaominhan analysisofbeauvericininisariacicadaemiquelcultureondifferentmedia
AT jingzhang analysisofbeauvericininisariacicadaemiquelcultureondifferentmedia
AT fengqinli analysisofbeauvericininisariacicadaemiquelcultureondifferentmedia