Immobilization of Penicillin G Acylase on Vinyl Sulfone-Agarose: An Unexpected Effect of the Ionic Strength on the Performance of the Immobilization Process

Immobilization of Penicillin G Acylase on Vinyl Sulfone-Agarose: An Unexpected Effect of the Ionic Strength on the Performance of the Immobilization Process

Penicillin G acylase (PGA) from <i>Escherichia coli</i> was immobilized on vinyl sulfone (VS) agarose. The immobilization of the enzyme failed at all pH values using 50 mM of buffer, while the progressive increase of ionic strength permitted its rapid immobilization under all studied pH...

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Bibliographic Details
Main Authors:	Thays N. da Rocha, Roberto Morellon-Sterlling, Javier Rocha-Martin, Juan M. Bolivar, Luciana R. B. Gonçalves, Roberto Fernandez-Lafuente
Format:	Article
Language:	English
Published:	MDPI AG 2022-11-01
Series:	Molecules
Subjects:	enzyme immobilization/stabilization heterofunctional supports multipoint covalent attachment immobilization optimization multi-step immobilization vinyl sulfone supports
Online Access:	https://www.mdpi.com/1420-3049/27/21/7587

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